Restriction map for
pFN21AE3110
(Linear) (Six-base) MAPSORT of: KIEE3110.seq Check: 2767 from: 1 to: 843
KIEE3110.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 843
Size: 222 621
AflIII A'CryG_T
Cuts at: 0 206 843
Size: 206 637
AleI CACnn'nnGTG
Cuts at: 0 613 843
Size: 613 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 843
Size: 631 212
ApaLI G'TGCA_C
Cuts at: 0 616 843
Size: 616 227
BamHI G'GATC_C
Cuts at: 0 266 843
Size: 266 577
BanII G_rGCy'C
Cuts at: 0 7 200 805 843
Size: 7 193 605 38
Fragments arranged by size:
605 193 38 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 359 843
Size: 325 34 484
Fragments arranged by size:
484 325 34
BglI GCCn_nnn'nGGC
Cuts at: 0 736 843
Size: 736 107
BglII A'GATC_T
Cuts at: 0 545 836 843
Size: 545 291 7
Fragments arranged by size:
545 291 7
Bme1580I G_kGCm'C
Cuts at: 0 620 843
Size: 620 223
BmgBI CAC'GTC
Cuts at: 0 72 209 843
Size: 72 137 634
Fragments arranged by size:
634 137 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 214 234 570 843
Size: 112 102 20 336 273
Fragments arranged by size:
336 273 112 102 20
Bpu10I CC'TnA_GC
Cuts at: 0 260 843
Size: 260 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 649 754 843
Size: 28 621 105 89
Fragments arranged by size:
621 105 89 28
BsaWI w'CCGG_w
Cuts at: 0 18 843
Size: 18 825
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 367 591 843
Size: 213 154 224 252
Fragments arranged by size:
252 224 213 154
BsiEI CG_ry'CG
Cuts at: 0 180 318 714 843
Size: 180 138 396 129
Fragments arranged by size:
396 180 138 129
BsiHKAI G_wGCw'C
Cuts at: 0 200 524 620 843
Size: 200 324 96 223
Fragments arranged by size:
324 223 200 96
BsiWI C'GTAC_G
Cuts at: 0 125 428 843
Size: 125 303 415
Fragments arranged by size:
415 303 125
BsmBI CGTCTCn'nnnn_
Cuts at: 0 606 843
Size: 606 237
Bsp1286I G_dGCh'C
Cuts at: 0 7 200 524 620 805 843
Size: 7 193 324 96 185 38
Fragments arranged by size:
324 193 185 96 38 7
BspHI T'CATG_A
Cuts at: 0 701 843
Size: 701 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 393 843
Size: 47 346 450
Fragments arranged by size:
450 346 47
BsrFI r'CCGG_y
Cuts at: 0 179 221 377 843
Size: 179 42 156 466
Fragments arranged by size:
466 179 156 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 843
Size: 171 672
BstYI r'GATC_y
Cuts at: 0 86 266 362 545 836 843
Size: 86 180 96 183 291 7
Fragments arranged by size:
291 183 180 96 86 7
BtsI GCAGTG_nn'
Cuts at: 0 637 843
Size: 637 206
ClaI AT'CG_AT
Cuts at: 0 335 843
Size: 335 508
EaeI y'GGCC_r
Cuts at: 0 177 680 711 728 843
Size: 177 503 31 17 115
Fragments arranged by size:
503 177 115 31 17
EagI C'GGCC_G
Cuts at: 0 177 711 843
Size: 177 534 132
Fragments arranged by size:
534 177 132
EarI CTCTTCn'nnn_
Cuts at: 0 19 843
Size: 19 824
EcoICRI GAG'CTC
Cuts at: 0 198 843
Size: 198 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 214 222 234 570 843
Size: 112 102 8 12 336 273
Fragments arranged by size:
336 273 112 102 12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 249 843
Size: 249 594
EcoO109I rG'GnC_Cy
Cuts at: 0 242 405 579 843
Size: 242 163 174 264
Fragments arranged by size:
264 242 174 163
HaeII r_GCGC'y
Cuts at: 0 260 843
Size: 260 583
HindIII A'AGCT_T
Cuts at: 0 631 843
Size: 631 212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 843
Size: 351 492
MscI TGG'CCA
Cuts at: 0 682 730 843
Size: 682 48 113
Fragments arranged by size:
682 113 48
MslI CAynn'nnrTG
Cuts at: 0 613 843
Size: 613 230
MspA1I CmG'CkG
Cuts at: 0 296 320 734 843
Size: 296 24 414 109
Fragments arranged by size:
414 296 109 24
NaeI GCC'GGC
Cuts at: 0 181 843
Size: 181 662
NgoMIV G'CCGG_C
Cuts at: 0 179 843
Size: 179 664
NspI r_CATG'y
Cuts at: 0 346 843
Size: 346 497
PflMI CCAn_nnn'nTGG
Cuts at: 0 296 843
Size: 296 547
PfoI T'CCnGG_A
Cuts at: 0 89 843
Size: 89 754
PpuMI rG'GwC_Cy
Cuts at: 0 242 405 579 843
Size: 242 163 174 264
Fragments arranged by size:
264 242 174 163
PstI C_TGCA'G
Cuts at: 0 104 578 843
Size: 104 474 265
Fragments arranged by size:
474 265 104
SacI G_AGCT'C
Cuts at: 0 200 843
Size: 200 643
SapI GCTCTTCn'nnn_
Cuts at: 0 19 843
Size: 19 824
SbfI CC_TGCA'GG
Cuts at: 0 104 578 843
Size: 104 474 265
Fragments arranged by size:
474 265 104
SfcI C'TryA_G
Cuts at: 0 100 132 574 843
Size: 100 32 442 269
Fragments arranged by size:
442 269 100 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 736 843
Size: 736 107
SmlI C'TyrA_G
Cuts at: 0 43 664 769 843
Size: 43 621 105 74
Fragments arranged by size:
621 105 74 43
SphI G_CATG'C
Cuts at: 0 346 843
Size: 346 497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 652 684 700 732 843
Size: 338 32 282 32 16 32 111
Fragments arranged by size:
338 282 111 32 32 32 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 843
Size: 168 675
Enzymes that do cut:
AcuI AflIII AleI AlfI ApaLI BamHI BanII BcgI
BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI
BsrFI BstXI BstYI BtsI ClaI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII HindIII MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PflMI PfoI PpuMI
PstI SacI SapI SbfI SfcI SfiI SmlI SphI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BlpI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsmI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI ScaI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI