Restriction map for
pFN21AE3108
(Linear) (Six-base) MAPSORT of: KIEE3108.seq Check: 6106 from: 1 to: 831
KIEE3108.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 467 831
Size: 467 364
AatII G_ACGT'C
Cuts at: 0 491 831
Size: 491 340
AccI GT'mk_AC
Cuts at: 0 109 386 831
Size: 109 277 445
Fragments arranged by size:
445 277 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 831
Size: 322 509
AflIII A'CryG_T
Cuts at: 0 449 831
Size: 449 382
AleI CACnn'nnGTG
Cuts at: 0 568 831
Size: 568 263
AlwNI CAG_nnn'CTG
Cuts at: 0 549 831
Size: 549 282
BanI G'GyrC_C
Cuts at: 0 333 713 831
Size: 333 380 118
Fragments arranged by size:
380 333 118
BanII G_rGCy'C
Cuts at: 0 34 831
Size: 34 797
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 831
Size: 26 805
BclI T'GATC_A
Cuts at: 0 623 831
Size: 623 208
BmgBI CAC'GTC
Cuts at: 0 399 519 831
Size: 399 120 312
Fragments arranged by size:
399 312 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 831
Size: 559 272
Bpu10I CC'TnA_GC
Cuts at: 0 184 831
Size: 184 647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 831
Size: 345 486
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 831
Size: 367 464
BsaHI Gr'CG_yC
Cuts at: 0 488 831
Size: 488 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 831
Size: 466 30 335
Fragments arranged by size:
466 335 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 650 734 831
Size: 601 49 84 97
Fragments arranged by size:
601 97 84 49
BseYI C'CCAG_C
Cuts at: 0 138 831
Size: 138 693
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 831
Size: 691 140
BsiEI CG_ry'CG
Cuts at: 0 51 113 831
Size: 51 62 718
Fragments arranged by size:
718 62 51
Bsp1286I G_dGCh'C
Cuts at: 0 34 831
Size: 34 797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 467 831
Size: 467 364
BsrBI CCG'CTC
Cuts at: 0 86 831
Size: 86 745
BsrFI r'CCGG_y
Cuts at: 0 576 831
Size: 576 255
BstEII G'GTnAC_C
Cuts at: 0 365 831
Size: 365 466
BstYI r'GATC_y
Cuts at: 0 440 831
Size: 440 391
BtgI C'CryG_G
Cuts at: 0 377 831
Size: 377 454
BtsI GCAGTG_nn'
Cuts at: 0 92 831
Size: 92 739
DrdI GACnn_nn'nnGTC
Cuts at: 0 487 831
Size: 487 344
EarI CTCTTCn'nnn_
Cuts at: 0 472 498 831
Size: 472 26 333
Fragments arranged by size:
472 333 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 750 831
Size: 750 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 559 831
Size: 322 237 272
Fragments arranged by size:
322 272 237
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 831
Size: 187 644
EcoO109I rG'GnC_Cy
Cuts at: 0 207 534 831
Size: 207 327 297
Fragments arranged by size:
327 297 207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 597 768 831
Size: 597 171 63
Fragments arranged by size:
597 171 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 831
Size: 466 32 333
Fragments arranged by size:
466 333 32
HincII GTy'rAC
Cuts at: 0 110 831
Size: 110 721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 831
Size: 509 322
MslI CAynn'nnrTG
Cuts at: 0 266 421 568 831
Size: 266 155 147 263
Fragments arranged by size:
266 263 155 147
MspA1I CmG'CkG
Cuts at: 0 63 101 162 168 831
Size: 63 38 61 6 663
Fragments arranged by size:
663 63 61 38 6
NaeI GCC'GGC
Cuts at: 0 578 831
Size: 578 253
NgoMIV G'CCGG_C
Cuts at: 0 576 831
Size: 576 255
NspI r_CATG'y
Cuts at: 0 453 831
Size: 453 378
PasI CC'CwG_GG
Cuts at: 0 775 831
Size: 775 56
PciI A'CATG_T
Cuts at: 0 449 831
Size: 449 382
PfoI T'CCnGG_A
Cuts at: 0 443 512 680 831
Size: 443 69 168 151
Fragments arranged by size:
443 168 151 69
PpuMI rG'GwC_Cy
Cuts at: 0 207 534 831
Size: 207 327 297
Fragments arranged by size:
327 297 207
SalI G'TCGA_C
Cuts at: 0 108 831
Size: 108 723
SfcI C'TryA_G
Cuts at: 0 429 831
Size: 429 402
SmlI C'TyrA_G
Cuts at: 0 360 831
Size: 360 471
StuI AGG'CCT
Cuts at: 0 235 831
Size: 235 596
StyI C'CwwG_G
Cuts at: 0 230 831
Size: 230 601
TatI w'GTAC_w
Cuts at: 0 248 557 831
Size: 248 309 274
Fragments arranged by size:
309 274 248
ZraI GAC'GTC
Cuts at: 0 489 831
Size: 489 342
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlwNI BanI
BanII BbsI BclI BmgBI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrBI BsrFI BstEII BstYI BtgI BtsI DrdI EarI
EciI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MmeI
MslI MspA1I NaeI NgoMIV NspI PasI PciI PfoI
PpuMI SalI SfcI SmlI StuI StyI TatI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BsaAI BsaBI
BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EaeI EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI