Restriction map for
pFN21AE3107
(Linear) (Six-base) MAPSORT of: KIEE3107.seq Check: 1473 from: 1 to: 831
KIEE3107.orf
With 182 enzymes: SgfI *
July 3, 2012 17:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 831
Size: 27 804
AleI CACnn'nnGTG
Cuts at: 0 160 831
Size: 160 671
AlwNI CAG_nnn'CTG
Cuts at: 0 211 831
Size: 211 620
ApaI G_GGCC'C
Cuts at: 0 113 831
Size: 113 718
AvaI C'yCGr_G
Cuts at: 0 67 831
Size: 67 764
BanI G'GyrC_C
Cuts at: 0 244 831
Size: 244 587
BanII G_rGCy'C
Cuts at: 0 64 113 374 831
Size: 64 49 261 457
Fragments arranged by size:
457 261 64 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 797 831
Size: 797 34
BglI GCCn_nnn'nGGC
Cuts at: 0 117 831
Size: 117 714
BglII A'GATC_T
Cuts at: 0 413 831
Size: 413 418
Bme1580I G_kGCm'C
Cuts at: 0 113 831
Size: 113 718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 388 559 831
Size: 69 319 171 272
Fragments arranged by size:
319 272 171 69
BsaAI yAC'GTr
Cuts at: 0 198 831
Size: 198 633
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 640 643 831
Size: 72 568 3 188
Fragments arranged by size:
568 188 72 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 243 831
Size: 79 164 588
Fragments arranged by size:
588 164 79
BsiHKAI G_wGCw'C
Cuts at: 0 39 831
Size: 39 792
BsmI GAATG_Cn'
Cuts at: 0 516 831
Size: 516 315
Bsp1286I G_dGCh'C
Cuts at: 0 39 64 113 374 831
Size: 39 25 49 261 457
Fragments arranged by size:
457 261 49 39 25
BspHI T'CATG_A
Cuts at: 0 797 831
Size: 797 34
BsrDI GCAATG_nn'
Cuts at: 0 137 831
Size: 137 694
BsrGI T'GTAC_A
Cuts at: 0 431 831
Size: 431 400
BstXI CCAn_nnnn'nTGG
Cuts at: 0 241 274 314 831
Size: 241 33 40 517
Fragments arranged by size:
517 241 40 33
BstYI r'GATC_y
Cuts at: 0 413 831
Size: 413 418
BtgI C'CryG_G
Cuts at: 0 5 50 831
Size: 5 45 781
Fragments arranged by size:
781 45 5
BtsI GCAGTG_nn'
Cuts at: 0 260 831
Size: 260 571
EaeI y'GGCC_r
Cuts at: 0 2 53 669 831
Size: 2 51 616 162
Fragments arranged by size:
616 162 51 2
EarI CTCTTCn'nnn_
Cuts at: 0 595 831
Size: 595 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 69 388 559 831
Size: 27 42 319 171 272
Fragments arranged by size:
319 272 171 42 27
EcoO109I rG'GnC_Cy
Cuts at: 0 110 831
Size: 110 721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 703 729 831
Size: 703 26 102
Fragments arranged by size:
703 102 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 831
Size: 728 103
MscI TGG'CCA
Cuts at: 0 55 671 831
Size: 55 616 160
Fragments arranged by size:
616 160 55
MslI CAynn'nnrTG
Cuts at: 0 142 160 736 831
Size: 142 18 576 95
Fragments arranged by size:
576 142 95 18
MspA1I CmG'CkG
Cuts at: 0 7 366 663 831
Size: 7 359 297 168
Fragments arranged by size:
359 297 168 7
NcoI C'CATG_G
Cuts at: 0 50 831
Size: 50 781
PspOMI G'GGCC_C
Cuts at: 0 109 831
Size: 109 722
PspXI vC'TCGA_Gb
Cuts at: 0 67 831
Size: 67 764
PvuII CAG'CTG
Cuts at: 0 366 663 831
Size: 366 297 168
Fragments arranged by size:
366 297 168
SacII CC_GC'GG
Cuts at: 0 8 831
Size: 8 823
SfcI C'TryA_G
Cuts at: 0 203 212 831
Size: 203 9 619
Fragments arranged by size:
619 203 9
SmlI C'TyrA_G
Cuts at: 0 67 831
Size: 67 764
StuI AGG'CCT
Cuts at: 0 92 831
Size: 92 739
StyI C'CwwG_G
Cuts at: 0 50 831
Size: 50 781
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 831
Size: 262 569
TatI w'GTAC_w
Cuts at: 0 360 408 431 831
Size: 360 48 23 400
Fragments arranged by size:
400 360 48 23
XbaI T'CTAG_A
Cuts at: 0 654 831
Size: 654 177
XhoI C'TCGA_G
Cuts at: 0 67 831
Size: 67 764
Enzymes that do cut:
AcuI AleI AlwNI ApaI AvaI BanI BanII BbsI
BglI BglII Bme1580I BpmI BsaAI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI BtgI
BtsI EaeI EarI Eco57MI EcoO109I FalI MmeI MscI
MslI MspA1I NcoI PspOMI PspXI PvuII SacII SfcI
SmlI StuI StyI TaqII TatI XbaI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XcmI XmaI XmnI ZraI