Restriction map for
pFN21AE3106
(Linear) (Six-base) MAPSORT of: KIEE3106.seq Check: 1756 from: 1 to: 828
KIEE3106.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 565 828
Size: 565 263
AfeI AGC'GCT
Cuts at: 0 115 828
Size: 115 713
AlwNI CAG_nnn'CTG
Cuts at: 0 813 828
Size: 813 15
ApaI G_GGCC'C
Cuts at: 0 126 187 828
Size: 126 61 641
Fragments arranged by size:
641 126 61
AvaI C'yCGr_G
Cuts at: 0 76 180 502 828
Size: 76 104 322 326
Fragments arranged by size:
326 322 104 76
BanI G'GyrC_C
Cuts at: 0 22 71 828
Size: 22 49 757
Fragments arranged by size:
757 49 22
BanII G_rGCy'C
Cuts at: 0 126 187 219 389 443 828
Size: 126 61 32 170 54 385
Fragments arranged by size:
385 170 126 61 54 32
BbeI G_GCGC'C
Cuts at: 0 26 75 828
Size: 26 49 753
Fragments arranged by size:
753 49 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 756 828
Size: 756 72
BbvCI CC'TCA_GC
Cuts at: 0 576 828
Size: 576 252
BglI GCCn_nnn'nGGC
Cuts at: 0 31 40 268 828
Size: 31 9 228 560
Fragments arranged by size:
560 228 31 9
BlpI GC'TnA_GC
Cuts at: 0 110 828
Size: 110 718
Bme1580I G_kGCm'C
Cuts at: 0 52 126 187 569 626 828
Size: 52 74 61 382 57 202
Fragments arranged by size:
382 202 74 61 57 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 314 828
Size: 314 514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 828
Size: 454 374
Bpu10I CC'TnA_GC
Cuts at: 0 526 576 828
Size: 526 50 252
Fragments arranged by size:
526 252 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 394 529 828
Size: 394 135 299
Fragments arranged by size:
394 299 135
BsaHI Gr'CG_yC
Cuts at: 0 23 64 72 461 828
Size: 23 41 8 389 367
Fragments arranged by size:
389 367 41 23 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 227 385 547 688 828
Size: 94 133 158 162 141 140
Fragments arranged by size:
162 158 141 140 133 94
BseYI C'CCAG_C
Cuts at: 0 331 417 570 828
Size: 331 86 153 258
Fragments arranged by size:
331 258 153 86
BsiEI CG_ry'CG
Cuts at: 0 784 828
Size: 784 44
Bsp1286I G_dGCh'C
Cuts at: 0 52 126 187 219 389 443 569 626
Size: 52 74 61 32 170 54 126 57
Cuts at: 626 828
Size: 202
Fragments arranged by size:
202 170 126 74 61 57 54 52
32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 565 782 828
Size: 565 217 46
Fragments arranged by size:
565 217 46
BsrFI r'CCGG_y
Cuts at: 0 30 544 828
Size: 30 514 284
Fragments arranged by size:
514 284 30
BssHII G'CGCG_C
Cuts at: 0 232 726 728 828
Size: 232 494 2 100
Fragments arranged by size:
494 232 100 2
BtgI C'CryG_G
Cuts at: 0 95 155 204 778 784 828
Size: 95 60 49 574 6 44
Fragments arranged by size:
574 95 60 49 44 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 237 696 828
Size: 237 459 132
Fragments arranged by size:
459 237 132
BtsI GCAGTG_nn'
Cuts at: 0 275 828
Size: 275 553
DrdI GACnn_nn'nnGTC
Cuts at: 0 760 828
Size: 760 68
EaeI y'GGCC_r
Cuts at: 0 32 353 781 828
Size: 32 321 428 47
Fragments arranged by size:
428 321 47 32
EagI C'GGCC_G
Cuts at: 0 781 828
Size: 781 47
EarI CTCTTCn'nnn_
Cuts at: 0 640 696 828
Size: 640 56 132
Fragments arranged by size:
640 132 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 238 828
Size: 238 590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 828
Size: 454 374
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 529 828
Size: 223 306 299
Fragments arranged by size:
306 299 223
EcoO109I rG'GnC_Cy
Cuts at: 0 184 474 771 828
Size: 184 290 297 57
Fragments arranged by size:
297 290 184 57
FseI GG_CCGG'CC
Cuts at: 0 34 828
Size: 34 794
HaeII r_GCGC'y
Cuts at: 0 26 75 117 828
Size: 26 49 42 711
Fragments arranged by size:
711 49 42 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 828
Size: 646 32 150
Fragments arranged by size:
646 150 32
KasI G'GCGC_C
Cuts at: 0 22 71 828
Size: 22 49 757
Fragments arranged by size:
757 49 22
MscI TGG'CCA
Cuts at: 0 355 828
Size: 355 473
MspA1I CmG'CkG
Cuts at: 0 109 157 417 786 828
Size: 109 48 260 369 42
Fragments arranged by size:
369 260 109 48 42
NaeI GCC'GGC
Cuts at: 0 32 546 828
Size: 32 514 282
Fragments arranged by size:
514 282 32
NarI GG'CG_CC
Cuts at: 0 23 72 828
Size: 23 49 756
Fragments arranged by size:
756 49 23
NcoI C'CATG_G
Cuts at: 0 204 828
Size: 204 624
NgoMIV G'CCGG_C
Cuts at: 0 30 544 828
Size: 30 514 284
Fragments arranged by size:
514 284 30
NspI r_CATG'y
Cuts at: 0 726 828
Size: 726 102
PfoI T'CCnGG_A
Cuts at: 0 470 828
Size: 470 358
PpuMI rG'GwC_Cy
Cuts at: 0 474 771 828
Size: 474 297 57
Fragments arranged by size:
474 297 57
PspOMI G'GGCC_C
Cuts at: 0 122 183 828
Size: 122 61 645
Fragments arranged by size:
645 122 61
PvuII CAG'CTG
Cuts at: 0 417 828
Size: 417 411
SacII CC_GC'GG
Cuts at: 0 158 787 828
Size: 158 629 41
Fragments arranged by size:
629 158 41
SfoI GGC'GCC
Cuts at: 0 24 73 828
Size: 24 49 755
Fragments arranged by size:
755 49 24
SmaI CCC'GGG
Cuts at: 0 182 828
Size: 182 646
SphI G_CATG'C
Cuts at: 0 726 828
Size: 726 102
SrfI GCCC'GGGC
Cuts at: 0 182 828
Size: 182 646
StyI C'CwwG_G
Cuts at: 0 204 828
Size: 204 624
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 345 772 828
Size: 333 12 427 56
Fragments arranged by size:
427 333 56 12
TatI w'GTAC_w
Cuts at: 0 508 828
Size: 508 320
XmaI C'CCGG_G
Cuts at: 0 180 828
Size: 180 648
Enzymes that do cut:
AarI AfeI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BsaI BsaHI BseRI BseYI BsiEI Bsp1286I BspMI BsrFI
BssHII BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI
MscI MspA1I NaeI NarI NcoI NgoMIV NspI PfoI
PpuMI PspOMI PvuII SacII SfoI SmaI SphI SrfI
StyI TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoICRI
EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmlI SnaBI SpeI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI