(Linear) (Six-base) MAPSORT of: KIEE3104.seq  Check: 6070  from: 1  to: 825



KIEE3104.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     443     825 
   Size:        443     382
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     395     825 
   Size:        395     430
 
ApoI r'AATT_y

Cuts at:      0      55     825 
   Size:         55     770
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     104     825 
   Size:        104     721
 
Bme1580I G_kGCm'C

Cuts at:      0     250     825 
   Size:        250     575
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     357     825 
   Size:        357     468
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     506     561     825 
   Size:        506      55     264

  Fragments arranged by size: 

                 506     264      55
 
BsaBI GATnn'nnATC

Cuts at:      0     409     825 
   Size:        409     416
 
BsmI GAATG_Cn'

Cuts at:      0     384     825 
   Size:        384     441
 
Bsp1286I G_dGCh'C

Cuts at:      0     250     825 
   Size:        250     575
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     262     451     825 
   Size:        262     189     374

  Fragments arranged by size: 

                 374     262     189
 
BsrDI GCAATG_nn'

Cuts at:      0     620     825 
   Size:        620     205
 
BstYI r'GATC_y

Cuts at:      0     404     825 
   Size:        404     421
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     271     306     825 
   Size:        271      35     519

  Fragments arranged by size: 

                 519     271      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      47     825 
   Size:         47     778
 
EaeI y'GGCC_r

Cuts at:      0      15     132     825 
   Size:         15     117     693

  Fragments arranged by size: 

                 693     117      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     443     825 
   Size:        443     382
 
EcoRI G'AATT_C

Cuts at:      0      55     825 
   Size:         55     770
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     138     825 
   Size:        138     687
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     505     537     584     616     825 
   Size:        505      32      47      32     209

  Fragments arranged by size: 

                 505     209      47      32      32
 
HincII GTy'rAC

Cuts at:      0     306     825 
   Size:        306     519
 
HpaI GTT'AAC

Cuts at:      0     306     825 
   Size:        306     519
 
MscI TGG'CCA

Cuts at:      0      17     825 
   Size:         17     808
 
MslI CAynn'nnrTG

Cuts at:      0     299     825 
   Size:        299     526
 
NdeI CA'TA_TG

Cuts at:      0     437     825 
   Size:        437     388
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     741     825 
   Size:        741      84
 
ScaI AGT'ACT

Cuts at:      0     118     825 
   Size:        118     707
 
StyI C'CwwG_G

Cuts at:      0     289     825 
   Size:        289     536
 
TatI w'GTAC_w

Cuts at:      0     116     317     825 
   Size:        116     201     508

  Fragments arranged by size: 

                 508     201     116
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BbsI Bme1580I     BmrI     BsaI    BsaBI
     BsmI Bsp1286I    BspMI    BsrDI    BstYI    CspCI   DraIII     EaeI
  Eco57MI    EcoRI     FalI    Hin4I   HincII     HpaI     MscI     MslI
     NdeI    PflMI     ScaI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI