Restriction map for
pFN21AE3095
(Linear) (Six-base) MAPSORT of: KIEE3095.seq Check: 1694 from: 1 to: 789
KIEE3095.orf
With 182 enzymes: SgfI *
March 22, 2012 16:13 ..
AflIII A'CryG_T
Cuts at: 0 479 789
Size: 479 310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 789
Size: 649 140
ApaI G_GGCC'C
Cuts at: 0 568 789
Size: 568 221
ApoI r'AATT_y
Cuts at: 0 380 712 789
Size: 380 332 77
Fragments arranged by size:
380 332 77
BanII G_rGCy'C
Cuts at: 0 568 618 789
Size: 568 50 171
Fragments arranged by size:
568 171 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 789
Size: 275 514
Bme1580I G_kGCm'C
Cuts at: 0 568 789
Size: 568 221
BmgBI CAC'GTC
Cuts at: 0 537 789
Size: 537 252
BmrI ACTGGGnnnn_n'
Cuts at: 0 698 789
Size: 698 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 789
Size: 467 322
BsaBI GATnn'nnATC
Cuts at: 0 35 91 789
Size: 35 56 698
Fragments arranged by size:
698 56 35
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 789
Size: 466 323
BseYI C'CCAG_C
Cuts at: 0 370 789
Size: 370 419
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 504 789
Size: 100 404 285
Fragments arranged by size:
404 285 100
BsiHKAI G_wGCw'C
Cuts at: 0 618 789
Size: 618 171
Bsp1286I G_dGCh'C
Cuts at: 0 568 618 789
Size: 568 50 171
Fragments arranged by size:
568 171 50
BspHI T'CATG_A
Cuts at: 0 353 789
Size: 353 436
BsrDI GCAATG_nn'
Cuts at: 0 691 789
Size: 691 98
Bsu36I CC'TnA_GG
Cuts at: 0 740 789
Size: 740 49
BtgI C'CryG_G
Cuts at: 0 243 330 651 789
Size: 243 87 321 138
Fragments arranged by size:
321 243 138 87
BtsI GCAGTG_nn'
Cuts at: 0 329 492 789
Size: 329 163 297
Fragments arranged by size:
329 297 163
EaeI y'GGCC_r
Cuts at: 0 302 584 789
Size: 302 282 205
Fragments arranged by size:
302 282 205
EarI CTCTTCn'nnn_
Cuts at: 0 82 389 703 789
Size: 82 307 314 86
Fragments arranged by size:
314 307 86 82
EcoICRI GAG'CTC
Cuts at: 0 616 789
Size: 616 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 789
Size: 467 322
EcoO109I rG'GnC_Cy
Cuts at: 0 290 565 789
Size: 290 275 224
Fragments arranged by size:
290 275 224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 442 789
Size: 442 347
MscI TGG'CCA
Cuts at: 0 304 586 789
Size: 304 282 203
Fragments arranged by size:
304 282 203
MslI CAynn'nnrTG
Cuts at: 0 631 789
Size: 631 158
NcoI C'CATG_G
Cuts at: 0 243 330 651 789
Size: 243 87 321 138
Fragments arranged by size:
321 243 138 87
NspI r_CATG'y
Cuts at: 0 483 789
Size: 483 306
PciI A'CATG_T
Cuts at: 0 479 789
Size: 479 310
PpuMI rG'GwC_Cy
Cuts at: 0 290 789
Size: 290 499
PspOMI G'GGCC_C
Cuts at: 0 564 789
Size: 564 225
SacI G_AGCT'C
Cuts at: 0 618 789
Size: 618 171
SapI GCTCTTCn'nnn_
Cuts at: 0 703 789
Size: 703 86
StyI C'CwwG_G
Cuts at: 0 243 299 330 651 763 789
Size: 243 56 31 321 112 26
Fragments arranged by size:
321 243 112 56 31 26
TatI w'GTAC_w
Cuts at: 0 161 754 789
Size: 161 593 35
Fragments arranged by size:
593 161 35
Tth111I GACn'n_nGTC
Cuts at: 0 538 789
Size: 538 251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 442 789
Size: 442 347
Enzymes that do cut:
AflIII AlfI ApaI ApoI BanII BbsI Bme1580I BmgBI
BmrI BpmI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrDI Bsu36I BtgI BtsI EaeI EarI EcoICRI
Eco57MI EcoO109I FalI MscI MslI NcoI NspI PciI
PpuMI PspOMI SacI SapI StyI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI XbaI
XhoI XmaI XmnI ZraI