(Linear) (Six-base) MAPSORT of: KIEE3089.seq  Check: 3742  from: 1  to: 771



KIEE3089.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     534     771 
   Size:        534     237
 
AccI GT'mk_AC

Cuts at:      0     403     771 
   Size:        403     368
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     771 
   Size:        430     341
 
AleI CACnn'nnGTG

Cuts at:      0     187     771 
   Size:        187     584
 
BanII G_rGCy'C

Cuts at:      0     147     771 
   Size:        147     624
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     542     732     771 
   Size:        542     190      39

  Fragments arranged by size: 

                 542     190      39
 
BglI GCCn_nnn'nGGC

Cuts at:      0     301     771 
   Size:        301     470
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750     771 
   Size:        750      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     446     771 
   Size:        446     325
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      42     523     531     771 
   Size:         42     481       8     240

  Fragments arranged by size: 

                 481     240      42       8
 
BseYI C'CCAG_C

Cuts at:      0      34     435     771 
   Size:         34     401     336

  Fragments arranged by size: 

                 401     336      34
 
Bsp1286I G_dGCh'C

Cuts at:      0     147     771 
   Size:        147     624
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     534     570     771 
   Size:        534      36     201

  Fragments arranged by size: 

                 534     201      36
 
BsrBI CCG'CTC

Cuts at:      0      50      89     771 
   Size:         50      39     682

  Fragments arranged by size: 

                 682      50      39
 
BsrFI r'CCGG_y

Cuts at:      0      38     771 
   Size:         38     733
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     492     771 
   Size:        492     279
 
BstYI r'GATC_y

Cuts at:      0     347     771 
   Size:        347     424
 
BstZ17I GTA'TAC

Cuts at:      0     404     771 
   Size:        404     367
 
BtsI GCAGTG_nn'

Cuts at:      0     391     771 
   Size:        391     380
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     576     611     771 
   Size:        576      35     160

  Fragments arranged by size: 

                 576     160      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     449     727     771 
   Size:        449     278      44

  Fragments arranged by size: 

                 449     278      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     271     771 
   Size:        271     500
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     771 
   Size:        430     341
 
HaeII r_GCGC'y

Cuts at:      0      33     128     771 
   Size:         33      95     643

  Fragments arranged by size: 

                 643      95      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     194     226     529     561     771 
   Size:        194      32     303      32     210

  Fragments arranged by size: 

                 303     210     194      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     187     412     771 
   Size:        187     225     359

  Fragments arranged by size: 

                 359     225     187
 
MspA1I CmG'CkG

Cuts at:      0      81     113     239     381     771 
   Size:         81      32     126     142     390

  Fragments arranged by size: 

                 390     142     126      81      32
 
NdeI CA'TA_TG

Cuts at:      0     427     771 
   Size:        427     344
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     315     771 
   Size:        315     456
 
PstI C_TGCA'G

Cuts at:      0      86     386     771 
   Size:         86     300     385

  Fragments arranged by size: 

                 385     300      86
 
PvuII CAG'CTG

Cuts at:      0     239     381     771 
   Size:        239     142     390

  Fragments arranged by size: 

                 390     239     142
 
SfcI C'TryA_G

Cuts at:      0      82     382     771 
   Size:         82     300     389

  Fragments arranged by size: 

                 389     300      82
 
SmlI C'TyrA_G

Cuts at:      0     765     771 
   Size:        765       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     222     771 
   Size:        222     549
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     432     771 
   Size:        432     339
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    BanII     BbsI     BglI    BpuEI
     BsaI    BseRI    BseYI Bsp1286I    BspMI    BsrBI    BsrFI    BstXI
    BstYI  BstZ17I     BtsI    CspCI     EarI     EciI  Eco57MI    HaeII
    Hin4I     MslI   MspA1I     NdeI    PflMI     PstI    PvuII     SfcI
     SmlI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII   Bsu36I     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI