(Linear) (Six-base) MAPSORT of: KIEE3088.seq  Check: 9785  from: 1  to: 645



KIEE3088.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:13  ..

AflIII A'CryG_T

Cuts at:      0     408     645 
   Size:        408     237
 
ApoI r'AATT_y

Cuts at:      0     146     645 
   Size:        146     499
 
AvaI C'yCGr_G

Cuts at:      0     548     645 
   Size:        548      97
 
BmtI G_CTAG'C

Cuts at:      0     344     645 
   Size:        344     301
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     405     645 
   Size:        405     240
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     546     645 
   Size:        546      99
 
BsaAI yAC'GTr

Cuts at:      0     259     645 
   Size:        259     386
 
BspHI T'CATG_A

Cuts at:      0      63     645 
   Size:         63     582
 
BstYI r'GATC_y

Cuts at:      0     533     645 
   Size:        533     112
 
Bsu36I CC'TnA_GG

Cuts at:      0     568     645 
   Size:        568      77
 
BtgI C'CryG_G

Cuts at:      0     181     645 
   Size:        181     464
 
EaeI y'GGCC_r

Cuts at:      0     178     645 
   Size:        178     467
 
EarI CTCTTCn'nnn_

Cuts at:      0     634     645 
   Size:        634      11
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     614     645 
   Size:        614      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     568     600     645 
   Size:        568      32      45

  Fragments arranged by size: 

                 568      45      32
 
HincII GTy'rAC

Cuts at:      0     414     645 
   Size:        414     231
 
MfeI C'AATT_G

Cuts at:      0      11     645 
   Size:         11     634
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     645 
   Size:        566      79
 
MslI CAynn'nnrTG

Cuts at:      0      68     645 
   Size:         68     577
 
NheI G'CTAG_C

Cuts at:      0     340     645 
   Size:        340     305
 
NruI TCG'CGA

Cuts at:      0     169     645 
   Size:        169     476
 
NspI r_CATG'y

Cuts at:      0     412     645 
   Size:        412     233
 
PciI A'CATG_T

Cuts at:      0     408     645 
   Size:        408     237
 
PsiI TTA'TAA

Cuts at:      0     298     645 
   Size:        298     347
 
SapI GCTCTTCn'nnn_

Cuts at:      0     634     645 
   Size:        634      11
 
ScaI AGT'ACT

Cuts at:      0     594     645 
   Size:        594      51
 
SmlI C'TyrA_G

Cuts at:      0     525     548     645 
   Size:        525      23      97

  Fragments arranged by size: 

                 525      97      23
 
SnaBI TAC'GTA

Cuts at:      0     259     645 
   Size:        259     386
 
SspI AAT'ATT

Cuts at:      0     497     645 
   Size:        497     148
 
StyI C'CwwG_G

Cuts at:      0     287     392     645 
   Size:        287     105     253

  Fragments arranged by size: 

                 287     253     105
 
TatI w'GTAC_w

Cuts at:      0     592     645 
   Size:        592      53
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     195     645 
   Size:        195     450
 
XhoI C'TCGA_G

Cuts at:      0     548     645 
   Size:        548      97
 
XmnI GAAnn'nnTTC

Cuts at:      0     146     645 
   Size:        146     499
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI     BmtI     BplI    BpuEI    BsaAI    BspHI
    BstYI   Bsu36I     BtgI     EaeI     EarI     FalI    Hin4I   HincII
     MfeI     MmeI     MslI     NheI     NruI     NspI     PciI     PsiI
     SapI     ScaI     SmlI    SnaBI     SspI     StyI     TatI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XmaI     ZraI