Restriction map for
pFN21AE3086
(Linear) (Six-base) MAPSORT of: KIEE3086.seq Check: 8105 from: 1 to: 753
KIEE3086.orf
With 182 enzymes: SgfI *
March 22, 2012 16:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 753
Size: 340 413
AflIII A'CryG_T
Cuts at: 0 551 753
Size: 551 202
AgeI A'CCGG_T
Cuts at: 0 559 753
Size: 559 194
AleI CACnn'nnGTG
Cuts at: 0 235 562 691 753
Size: 235 327 129 62
Fragments arranged by size:
327 235 129 62
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 580 753
Size: 253 327 173
Fragments arranged by size:
327 253 173
AlwNI CAG_nnn'CTG
Cuts at: 0 336 753
Size: 336 417
ApaI G_GGCC'C
Cuts at: 0 662 753
Size: 662 91
ApaLI G'TGCA_C
Cuts at: 0 555 753
Size: 555 198
AvaI C'yCGr_G
Cuts at: 0 48 220 526 689 735 753
Size: 48 172 306 163 46 18
Fragments arranged by size:
306 172 163 48 46 18
BanI G'GyrC_C
Cuts at: 0 160 244 436 604 753
Size: 160 84 192 168 149
Fragments arranged by size:
192 168 160 149 84
BanII G_rGCy'C
Cuts at: 0 662 753
Size: 662 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 628 753
Size: 594 34 125
Fragments arranged by size:
594 125 34
BglI GCCn_nnn'nGGC
Cuts at: 0 28 581 753
Size: 28 553 172
Fragments arranged by size:
553 172 28
Bme1580I G_kGCm'C
Cuts at: 0 165 559 607 662 753
Size: 165 394 48 55 91
Fragments arranged by size:
394 165 91 55 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 715 753
Size: 715 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 753
Size: 45 708
BsaHI Gr'CG_yC
Cuts at: 0 43 483 753
Size: 43 440 270
Fragments arranged by size:
440 270 43
BsaWI w'CCGG_w
Cuts at: 0 559 753
Size: 559 194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 753
Size: 259 30 464
Fragments arranged by size:
464 259 30
BseYI C'CCAG_C
Cuts at: 0 240 326 458 753
Size: 240 86 132 295
Fragments arranged by size:
295 240 132 86
BsiEI CG_ry'CG
Cuts at: 0 90 753
Size: 90 663
BsiHKAI G_wGCw'C
Cuts at: 0 559 569 753
Size: 559 10 184
Fragments arranged by size:
559 184 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 490 753
Size: 490 263
Bsp1286I G_dGCh'C
Cuts at: 0 165 559 569 607 662 753
Size: 165 394 10 38 55 91
Fragments arranged by size:
394 165 91 55 38 10
BsrDI GCAATG_nn'
Cuts at: 0 597 753
Size: 597 156
BsrFI r'CCGG_y
Cuts at: 0 55 433 454 559 753
Size: 55 378 21 105 194
Fragments arranged by size:
378 194 105 55 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 443 753
Size: 443 310
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 753
Size: 475 278
Bsu36I CC'TnA_GG
Cuts at: 0 256 753
Size: 256 497
BtgI C'CryG_G
Cuts at: 0 191 233 422 576 633 753
Size: 191 42 189 154 57 120
Fragments arranged by size:
191 189 154 120 57 42
BtsI GCAGTG_nn'
Cuts at: 0 403 480 753
Size: 403 77 273
Fragments arranged by size:
403 273 77
ClaI AT'CG_AT
Cuts at: 0 716 753
Size: 716 37
DraIII CAC_nnn'GTG
Cuts at: 0 639 753
Size: 639 114
EaeI y'GGCC_r
Cuts at: 0 87 194 452 666 753
Size: 87 107 258 214 87
Fragments arranged by size:
258 214 107 87 87
EagI C'GGCC_G
Cuts at: 0 87 753
Size: 87 666
EarI CTCTTCn'nnn_
Cuts at: 0 706 753
Size: 706 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 340 753
Size: 45 295 413
Fragments arranged by size:
413 295 45
EcoO109I rG'GnC_Cy
Cuts at: 0 101 624 658 659 673 753
Size: 101 523 34 1 14 80
Fragments arranged by size:
523 101 80 34 14 1
HaeII r_GCGC'y
Cuts at: 0 700 753
Size: 700 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 698 730 753
Size: 259 32 407 32 23
Fragments arranged by size:
407 259 32 32 23
HincII GTy'rAC
Cuts at: 0 510 753
Size: 510 243
MslI CAynn'nnrTG
Cuts at: 0 235 562 691 753
Size: 235 327 129 62
Fragments arranged by size:
327 235 129 62
MspA1I CmG'CkG
Cuts at: 0 193 200 360 578 753
Size: 193 7 160 218 175
Fragments arranged by size:
218 193 175 160 7
NaeI GCC'GGC
Cuts at: 0 57 435 456 753
Size: 57 378 21 297
Fragments arranged by size:
378 297 57 21
NgoMIV G'CCGG_C
Cuts at: 0 55 433 454 753
Size: 55 378 21 299
Fragments arranged by size:
378 299 55 21
PasI CC'CwG_GG
Cuts at: 0 654 753
Size: 654 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 753
Size: 639 114
PshAI GACnn'nnGTC
Cuts at: 0 536 753
Size: 536 217
PspOMI G'GGCC_C
Cuts at: 0 658 753
Size: 658 95
PspXI vC'TCGA_Gb
Cuts at: 0 48 753
Size: 48 705
PstI C_TGCA'G
Cuts at: 0 416 753
Size: 416 337
PvuII CAG'CTG
Cuts at: 0 200 360 753
Size: 200 160 393
Fragments arranged by size:
393 200 160
SacII CC_GC'GG
Cuts at: 0 194 579 753
Size: 194 385 174
Fragments arranged by size:
385 194 174
SapI GCTCTTCn'nnn_
Cuts at: 0 706 753
Size: 706 47
SfcI C'TryA_G
Cuts at: 0 412 753
Size: 412 341
SgrAI Cr'CCGG_yG
Cuts at: 0 559 753
Size: 559 194
SmaI CCC'GGG
Cuts at: 0 222 737 753
Size: 222 515 16
Fragments arranged by size:
515 222 16
SmlI C'TyrA_G
Cuts at: 0 48 753
Size: 48 705
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 753
Size: 232 521
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 705 737 753
Size: 705 32 16
Fragments arranged by size:
705 32 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 447 753
Size: 69 378 306
Fragments arranged by size:
378 306 69
XhoI C'TCGA_G
Cuts at: 0 48 753
Size: 48 705
XmaI C'CCGG_G
Cuts at: 0 220 735 753
Size: 220 515 18
Fragments arranged by size:
515 220 18
Enzymes that do cut:
AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BcgI BglI Bme1580I BplI BpmI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtsI ClaI
DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I
HincII MslI MspA1I NaeI NgoMIV PasI PflMI PshAI
PspOMI PspXI PstI PvuII SacII SapI SfcI SgrAI
SmaI SmlI TaqII TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BseRI BsgI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI
CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PsrI PvuI RsrII SacI
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XmnI ZraI