(Linear) (Six-base) MAPSORT of: KIEE3086.seq Check: 8105 from: 1 to: 753 KIEE3086.orf With 182 enzymes: SgfI * March 22, 2012 16:12 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 753 Size: 340 413 AflIII A'CryG_T Cuts at: 0 551 753 Size: 551 202 AgeI A'CCGG_T Cuts at: 0 559 753 Size: 559 194 AleI CACnn'nnGTG Cuts at: 0 235 562 691 753 Size: 235 327 129 62 Fragments arranged by size: 327 235 129 62 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 253 580 753 Size: 253 327 173 Fragments arranged by size: 327 253 173 AlwNI CAG_nnn'CTG Cuts at: 0 336 753 Size: 336 417 ApaI G_GGCC'C Cuts at: 0 662 753 Size: 662 91 ApaLI G'TGCA_C Cuts at: 0 555 753 Size: 555 198 AvaI C'yCGr_G Cuts at: 0 48 220 526 689 735 753 Size: 48 172 306 163 46 18 Fragments arranged by size: 306 172 163 48 46 18 BanI G'GyrC_C Cuts at: 0 160 244 436 604 753 Size: 160 84 192 168 149 Fragments arranged by size: 192 168 160 149 84 BanII G_rGCy'C Cuts at: 0 662 753 Size: 662 91 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 594 628 753 Size: 594 34 125 Fragments arranged by size: 594 125 34 BglI GCCn_nnn'nGGC Cuts at: 0 28 581 753 Size: 28 553 172 Fragments arranged by size: 553 172 28 Bme1580I G_kGCm'C Cuts at: 0 165 559 607 662 753 Size: 165 394 48 55 91 Fragments arranged by size: 394 165 91 55 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 715 753 Size: 715 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 753 Size: 45 708 BsaHI Gr'CG_yC Cuts at: 0 43 483 753 Size: 43 440 270 Fragments arranged by size: 440 270 43 BsaWI w'CCGG_w Cuts at: 0 559 753 Size: 559 194 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 259 289 753 Size: 259 30 464 Fragments arranged by size: 464 259 30 BseYI C'CCAG_C Cuts at: 0 240 326 458 753 Size: 240 86 132 295 Fragments arranged by size: 295 240 132 86 BsiEI CG_ry'CG Cuts at: 0 90 753 Size: 90 663 BsiHKAI G_wGCw'C Cuts at: 0 559 569 753 Size: 559 10 184 Fragments arranged by size: 559 184 10 BsmBI CGTCTCn'nnnn_ Cuts at: 0 490 753 Size: 490 263 Bsp1286I G_dGCh'C Cuts at: 0 165 559 569 607 662 753 Size: 165 394 10 38 55 91 Fragments arranged by size: 394 165 91 55 38 10 BsrDI GCAATG_nn' Cuts at: 0 597 753 Size: 597 156 BsrFI r'CCGG_y Cuts at: 0 55 433 454 559 753 Size: 55 378 21 105 194 Fragments arranged by size: 378 194 105 55 21 BstAPI GCAn_nnn'nTGC Cuts at: 0 443 753 Size: 443 310 BstXI CCAn_nnnn'nTGG Cuts at: 0 475 753 Size: 475 278 Bsu36I CC'TnA_GG Cuts at: 0 256 753 Size: 256 497 BtgI C'CryG_G Cuts at: 0 191 233 422 576 633 753 Size: 191 42 189 154 57 120 Fragments arranged by size: 191 189 154 120 57 42 BtsI GCAGTG_nn' Cuts at: 0 403 480 753 Size: 403 77 273 Fragments arranged by size: 403 273 77 ClaI AT'CG_AT Cuts at: 0 716 753 Size: 716 37 DraIII CAC_nnn'GTG Cuts at: 0 639 753 Size: 639 114 EaeI y'GGCC_r Cuts at: 0 87 194 452 666 753 Size: 87 107 258 214 87 Fragments arranged by size: 258 214 107 87 87 EagI C'GGCC_G Cuts at: 0 87 753 Size: 87 666 EarI CTCTTCn'nnn_ Cuts at: 0 706 753 Size: 706 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 340 753 Size: 45 295 413 Fragments arranged by size: 413 295 45 EcoO109I rG'GnC_Cy Cuts at: 0 101 624 658 659 673 753 Size: 101 523 34 1 14 80 Fragments arranged by size: 523 101 80 34 14 1 HaeII r_GCGC'y Cuts at: 0 700 753 Size: 700 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 259 291 698 730 753 Size: 259 32 407 32 23 Fragments arranged by size: 407 259 32 32 23 HincII GTy'rAC Cuts at: 0 510 753 Size: 510 243 MslI CAynn'nnrTG Cuts at: 0 235 562 691 753 Size: 235 327 129 62 Fragments arranged by size: 327 235 129 62 MspA1I CmG'CkG Cuts at: 0 193 200 360 578 753 Size: 193 7 160 218 175 Fragments arranged by size: 218 193 175 160 7 NaeI GCC'GGC Cuts at: 0 57 435 456 753 Size: 57 378 21 297 Fragments arranged by size: 378 297 57 21 NgoMIV G'CCGG_C Cuts at: 0 55 433 454 753 Size: 55 378 21 299 Fragments arranged by size: 378 299 55 21 PasI CC'CwG_GG Cuts at: 0 654 753 Size: 654 99 PflMI CCAn_nnn'nTGG Cuts at: 0 639 753 Size: 639 114 PshAI GACnn'nnGTC Cuts at: 0 536 753 Size: 536 217 PspOMI G'GGCC_C Cuts at: 0 658 753 Size: 658 95 PspXI vC'TCGA_Gb Cuts at: 0 48 753 Size: 48 705 PstI C_TGCA'G Cuts at: 0 416 753 Size: 416 337 PvuII CAG'CTG Cuts at: 0 200 360 753 Size: 200 160 393 Fragments arranged by size: 393 200 160 SacII CC_GC'GG Cuts at: 0 194 579 753 Size: 194 385 174 Fragments arranged by size: 385 194 174 SapI GCTCTTCn'nnn_ Cuts at: 0 706 753 Size: 706 47 SfcI C'TryA_G Cuts at: 0 412 753 Size: 412 341 SgrAI Cr'CCGG_yG Cuts at: 0 559 753 Size: 559 194 SmaI CCC'GGG Cuts at: 0 222 737 753 Size: 222 515 16 Fragments arranged by size: 515 222 16 SmlI C'TyrA_G Cuts at: 0 48 753 Size: 48 705 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 232 753 Size: 232 521 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 705 737 753 Size: 705 32 16 Fragments arranged by size: 705 32 16 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 69 447 753 Size: 69 378 306 Fragments arranged by size: 378 306 69 XhoI C'TCGA_G Cuts at: 0 48 753 Size: 48 705 XmaI C'CCGG_G Cuts at: 0 220 735 753 Size: 220 515 18 Fragments arranged by size: 515 220 18 Enzymes that do cut: AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BcgI BglI Bme1580I BplI BpmI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtsI ClaI DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I HincII MslI MspA1I NaeI NgoMIV PasI PflMI PshAI PspOMI PspXI PstI PvuII SacII SapI SfcI SgrAI SmaI SmlI TaqII TstI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BsgI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI Tth111I XbaI XmnI ZraI