Restriction map for
pFN21AE3085
(Linear) (Six-base) MAPSORT of: KIEE3085.seq Check: 7744 from: 1 to: 750
KIEE3085.orf
With 182 enzymes: SgfI *
March 22, 2012 16:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 631 750
Size: 631 119
AlwNI CAG_nnn'CTG
Cuts at: 0 507 750
Size: 507 243
ApaI G_GGCC'C
Cuts at: 0 61 750
Size: 61 689
AvaI C'yCGr_G
Cuts at: 0 548 689 750
Size: 548 141 61
Fragments arranged by size:
548 141 61
BanII G_rGCy'C
Cuts at: 0 61 750
Size: 61 689
BbsI GAAGACnn'nnnn_
Cuts at: 0 639 750
Size: 639 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 242 750
Size: 242 508
Bme1580I G_kGCm'C
Cuts at: 0 61 750
Size: 61 689
BmgBI CAC'GTC
Cuts at: 0 519 750
Size: 519 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 750
Size: 55 695
BmtI G_CTAG'C
Cuts at: 0 28 750
Size: 28 722
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 750
Size: 334 416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 500 750
Size: 26 474 250
Fragments arranged by size:
474 250 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 750
Size: 361 30 359
Fragments arranged by size:
361 359 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 750
Size: 337 413
BsiHKAI G_wGCw'C
Cuts at: 0 320 750
Size: 320 430
Bsp1286I G_dGCh'C
Cuts at: 0 61 320 750
Size: 61 259 430
Fragments arranged by size:
430 259 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 631 750
Size: 631 119
BsrFI r'CCGG_y
Cuts at: 0 72 188 750
Size: 72 116 562
Fragments arranged by size:
562 116 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 457 478 750
Size: 457 21 272
Fragments arranged by size:
457 272 21
BstYI r'GATC_y
Cuts at: 0 295 750
Size: 295 455
Bsu36I CC'TnA_GG
Cuts at: 0 172 750
Size: 172 578
BtgI C'CryG_G
Cuts at: 0 122 134 530 750
Size: 122 12 396 220
Fragments arranged by size:
396 220 122 12
BtsI GCAGTG_nn'
Cuts at: 0 320 750
Size: 320 430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 500 750
Size: 26 474 250
Fragments arranged by size:
474 250 26
EcoO109I rG'GnC_Cy
Cuts at: 0 37 57 208 504 750
Size: 37 20 151 296 246
Fragments arranged by size:
296 246 151 37 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 446 478 750
Size: 446 32 272
Fragments arranged by size:
446 272 32
MspA1I CmG'CkG
Cuts at: 0 629 750
Size: 629 121
NaeI GCC'GGC
Cuts at: 0 74 190 750
Size: 74 116 560
Fragments arranged by size:
560 116 74
NcoI C'CATG_G
Cuts at: 0 134 530 750
Size: 134 396 220
Fragments arranged by size:
396 220 134
NgoMIV G'CCGG_C
Cuts at: 0 72 188 750
Size: 72 116 562
Fragments arranged by size:
562 116 72
NheI G'CTAG_C
Cuts at: 0 24 750
Size: 24 726
PfoI T'CCnGG_A
Cuts at: 0 248 298 470 750
Size: 248 50 172 280
Fragments arranged by size:
280 248 172 50
PpuMI rG'GwC_Cy
Cuts at: 0 504 750
Size: 504 246
PspOMI G'GGCC_C
Cuts at: 0 57 750
Size: 57 693
PstI C_TGCA'G
Cuts at: 0 167 197 750
Size: 167 30 553
Fragments arranged by size:
553 167 30
PvuII CAG'CTG
Cuts at: 0 629 750
Size: 629 121
RsrII CG'GwC_CG
Cuts at: 0 612 750
Size: 612 138
SexAI A'CCwGG_T
Cuts at: 0 356 750
Size: 356 394
SfcI C'TryA_G
Cuts at: 0 163 193 442 633 750
Size: 163 30 249 191 117
Fragments arranged by size:
249 191 163 117 30
SmaI CCC'GGG
Cuts at: 0 691 750
Size: 691 59
SmlI C'TyrA_G
Cuts at: 0 548 750
Size: 548 202
StuI AGG'CCT
Cuts at: 0 440 750
Size: 440 310
StyI C'CwwG_G
Cuts at: 0 134 380 450 530 558 660 750
Size: 134 246 70 80 28 102 90
Fragments arranged by size:
246 134 102 90 80 70 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 750
Size: 337 413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 750
Size: 537 213
XhoI C'TCGA_G
Cuts at: 0 548 750
Size: 548 202
XmaI C'CCGG_G
Cuts at: 0 689 750
Size: 689 61
Enzymes that do cut:
AarI AlwNI ApaI AvaI BanII BbsI BciVI Bme1580I
BmgBI BmrI BmtI BplI BpmI BsaXI BsgI BsiHKAI
Bsp1286I BspMI BsrFI BstXI BstYI Bsu36I BtgI BtsI
Eco57MI EcoO109I Hin4I MspA1I NaeI NcoI NgoMIV NheI
PfoI PpuMI PspOMI PstI PvuII RsrII SexAI SfcI
SmaI SmlI StuI StyI TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BfrBI BglI BglII BlpI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NarI NdeI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XmnI ZraI