Restriction map for
pFN21AE3084
(Linear) (Six-base) MAPSORT of: KIEE3084.seq Check: 7830 from: 1 to: 744
KIEE3084.orf
With 182 enzymes: SgfI *
March 22, 2012 16:12 ..
AflIII A'CryG_T
Cuts at: 0 344 744
Size: 344 400
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 744
Size: 526 32 186
Fragments arranged by size:
526 186 32
AlwNI CAG_nnn'CTG
Cuts at: 0 603 744
Size: 603 141
AvaI C'yCGr_G
Cuts at: 0 260 320 744
Size: 260 60 424
Fragments arranged by size:
424 260 60
BanI G'GyrC_C
Cuts at: 0 316 350 509 744
Size: 316 34 159 235
Fragments arranged by size:
316 235 159 34
BanII G_rGCy'C
Cuts at: 0 594 647 696 744
Size: 594 53 49 48
Fragments arranged by size:
594 53 49 48
BbeI G_GCGC'C
Cuts at: 0 320 744
Size: 320 424
BbsI GAAGACnn'nnnn_
Cuts at: 0 459 744
Size: 459 285
BclI T'GATC_A
Cuts at: 0 308 744
Size: 308 436
Bme1580I G_kGCm'C
Cuts at: 0 210 744
Size: 210 534
BmrI ACTGGGnnnn_n'
Cuts at: 0 408 440 744
Size: 408 32 304
Fragments arranged by size:
408 304 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 262 412 718 744
Size: 230 32 150 306 26
Fragments arranged by size:
306 230 150 32 26
Bpu10I CC'TnA_GC
Cuts at: 0 604 744
Size: 604 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 744
Size: 96 648
BsaHI Gr'CG_yC
Cuts at: 0 317 744
Size: 317 427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 526 556 691 721 744
Size: 166 30 330 30 135 30 23
Fragments arranged by size:
330 166 135 30 30 30 23
BseYI C'CCAG_C
Cuts at: 0 95 334 521 593 744
Size: 95 239 187 72 151
Fragments arranged by size:
239 187 151 95 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 425 744
Size: 96 329 319
Fragments arranged by size:
329 319 96
BsiHKAI G_wGCw'C
Cuts at: 0 84 744
Size: 84 660
BsmI GAATG_Cn'
Cuts at: 0 19 744
Size: 19 725
Bsp1286I G_dGCh'C
Cuts at: 0 84 210 594 647 696 744
Size: 84 126 384 53 49 48
Fragments arranged by size:
384 126 84 53 49 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 744
Size: 16 728
BsrBI CCG'CTC
Cuts at: 0 586 744
Size: 586 158
BsrGI T'GTAC_A
Cuts at: 0 341 744
Size: 341 403
BssSI C'ACGA_G
Cuts at: 0 355 744
Size: 355 389
BstXI CCAn_nnnn'nTGG
Cuts at: 0 46 744
Size: 46 698
BtgI C'CryG_G
Cuts at: 0 210 744
Size: 210 534
BtsI GCAGTG_nn'
Cuts at: 0 458 744
Size: 458 286
DraIII CAC_nnn'GTG
Cuts at: 0 357 744
Size: 357 387
EaeI y'GGCC_r
Cuts at: 0 27 744
Size: 27 717
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 408 744
Size: 408 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 262 412 718 744
Size: 230 32 150 306 26
Fragments arranged by size:
306 230 150 32 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 504 744
Size: 504 240
EcoO109I rG'GnC_Cy
Cuts at: 0 42 50 600 744
Size: 42 8 550 144
Fragments arranged by size:
550 144 42 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 744
Size: 561 183
HaeII r_GCGC'y
Cuts at: 0 227 320 744
Size: 227 93 424
Fragments arranged by size:
424 227 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 744
Size: 691 32 21
Fragments arranged by size:
691 32 21
HincII GTy'rAC
Cuts at: 0 192 744
Size: 192 552
KasI G'GCGC_C
Cuts at: 0 316 744
Size: 316 428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 744
Size: 197 547
MscI TGG'CCA
Cuts at: 0 29 744
Size: 29 715
MslI CAynn'nnrTG
Cuts at: 0 154 744
Size: 154 590
MspA1I CmG'CkG
Cuts at: 0 303 451 515 619 660 744
Size: 303 148 64 104 41 84
Fragments arranged by size:
303 148 104 84 64 41
NarI GG'CG_CC
Cuts at: 0 317 744
Size: 317 427
NdeI CA'TA_TG
Cuts at: 0 729 744
Size: 729 15
NspI r_CATG'y
Cuts at: 0 348 744
Size: 348 396
PciI A'CATG_T
Cuts at: 0 344 744
Size: 344 400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 744
Size: 526 32 186
Fragments arranged by size:
526 186 32
PpuMI rG'GwC_Cy
Cuts at: 0 50 744
Size: 50 694
PvuII CAG'CTG
Cuts at: 0 303 744
Size: 303 441
SanDI GG'GwC_CC
Cuts at: 0 50 744
Size: 50 694
SfoI GGC'GCC
Cuts at: 0 318 744
Size: 318 426
SmaI CCC'GGG
Cuts at: 0 262 322 744
Size: 262 60 422
Fragments arranged by size:
422 262 60
SmlI C'TyrA_G
Cuts at: 0 75 744
Size: 75 669
StyI C'CwwG_G
Cuts at: 0 45 652 744
Size: 45 607 92
Fragments arranged by size:
607 92 45
TatI w'GTAC_w
Cuts at: 0 341 744
Size: 341 403
XmaI C'CCGG_G
Cuts at: 0 260 320 744
Size: 260 60 424
Fragments arranged by size:
424 260 60
XmnI GAAnn'nnTTC
Cuts at: 0 541 544 744
Size: 541 3 200
Fragments arranged by size:
541 200 3
Enzymes that do cut:
AflIII AloI AlwNI AvaI BanI BanII BbeI BbsI
BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrGI
BssSI BstXI BtgI BtsI DraIII EaeI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NdeI NspI PciI PpiI
PpuMI PvuII SanDI SfoI SmaI SmlI StyI TatI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmBI BspEI
BspHI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI ZraI