Restriction map for
pFN21AE3083
(Linear) (Six-base) MAPSORT of: KIEE3083.seq Check: 3593 from: 1 to: 732
KIEE3083.orf
With 182 enzymes: SgfI *
March 22, 2012 16:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 292 732
Size: 292 440
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 732
Size: 159 573
AleI CACnn'nnGTG
Cuts at: 0 433 732
Size: 433 299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 451 732
Size: 451 281
AlwNI CAG_nnn'CTG
Cuts at: 0 30 90 732
Size: 30 60 642
Fragments arranged by size:
642 60 30
ApaI G_GGCC'C
Cuts at: 0 707 732
Size: 707 25
BanI G'GyrC_C
Cuts at: 0 344 732
Size: 344 388
BanII G_rGCy'C
Cuts at: 0 173 457 578 707 732
Size: 173 284 121 129 25
Fragments arranged by size:
284 173 129 121 25
Bme1580I G_kGCm'C
Cuts at: 0 707 732
Size: 707 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 639 732
Size: 639 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 732
Size: 523 209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 398 732
Size: 244 154 334
Fragments arranged by size:
334 244 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 732
Size: 655 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 613 732
Size: 486 127 119
Fragments arranged by size:
486 127 119
BsaWI w'CCGG_w
Cuts at: 0 241 479 732
Size: 241 238 253
Fragments arranged by size:
253 241 238
BseYI C'CCAG_C
Cuts at: 0 72 228 545 732
Size: 72 156 317 187
Fragments arranged by size:
317 187 156 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 411 469 732
Size: 40 371 58 263
Fragments arranged by size:
371 263 58 40
BsiHKAI G_wGCw'C
Cuts at: 0 173 445 509 732
Size: 173 272 64 223
Fragments arranged by size:
272 223 173 64
Bsp1286I G_dGCh'C
Cuts at: 0 173 445 457 509 578 707 732
Size: 173 272 12 52 69 129 25
Fragments arranged by size:
272 173 129 69 52 25 12
BspEI T'CCGG_A
Cuts at: 0 479 732
Size: 479 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 292 732
Size: 292 440
BsrBI CCG'CTC
Cuts at: 0 360 732
Size: 360 372
BstEII G'GTnAC_C
Cuts at: 0 279 732
Size: 279 453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 732
Size: 417 315
BstYI r'GATC_y
Cuts at: 0 630 732
Size: 630 102
BtgI C'CryG_G
Cuts at: 0 410 732
Size: 410 322
DrdI GACnn_nn'nnGTC
Cuts at: 0 10 732
Size: 10 722
EaeI y'GGCC_r
Cuts at: 0 119 732
Size: 119 613
EarI CTCTTCn'nnn_
Cuts at: 0 178 732
Size: 178 554
EcoICRI GAG'CTC
Cuts at: 0 171 732
Size: 171 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 244 398 732
Size: 159 85 154 334
Fragments arranged by size:
334 159 154 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 732
Size: 472 260
EcoO109I rG'GnC_Cy
Cuts at: 0 465 704 732
Size: 465 239 28
Fragments arranged by size:
465 239 28
MslI CAynn'nnrTG
Cuts at: 0 415 433 732
Size: 415 18 299
Fragments arranged by size:
415 299 18
MspA1I CmG'CkG
Cuts at: 0 27 396 732
Size: 27 369 336
Fragments arranged by size:
369 336 27
NcoI C'CATG_G
Cuts at: 0 410 732
Size: 410 322
NdeI CA'TA_TG
Cuts at: 0 537 732
Size: 537 195
NspI r_CATG'y
Cuts at: 0 526 732
Size: 526 206
PflMI CCAn_nnn'nTGG
Cuts at: 0 714 732
Size: 714 18
PfoI T'CCnGG_A
Cuts at: 0 205 375 732
Size: 205 170 357
Fragments arranged by size:
357 205 170
PpuMI rG'GwC_Cy
Cuts at: 0 465 732
Size: 465 267
PspOMI G'GGCC_C
Cuts at: 0 703 732
Size: 703 29
PstI C_TGCA'G
Cuts at: 0 26 277 473 732
Size: 26 251 196 259
Fragments arranged by size:
259 251 196 26
PvuII CAG'CTG
Cuts at: 0 27 396 732
Size: 27 369 336
Fragments arranged by size:
369 336 27
SacI G_AGCT'C
Cuts at: 0 173 732
Size: 173 559
SapI GCTCTTCn'nnn_
Cuts at: 0 178 732
Size: 178 554
SbfI CC_TGCA'GG
Cuts at: 0 473 732
Size: 473 259
ScaI AGT'ACT
Cuts at: 0 488 732
Size: 488 244
SfcI C'TryA_G
Cuts at: 0 22 273 469 732
Size: 22 251 196 263
Fragments arranged by size:
263 251 196 22
SmlI C'TyrA_G
Cuts at: 0 634 732
Size: 634 98
StyI C'CwwG_G
Cuts at: 0 410 457 732
Size: 410 47 275
Fragments arranged by size:
410 275 47
TatI w'GTAC_w
Cuts at: 0 486 732
Size: 486 246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 417 542 732
Size: 417 125 190
Fragments arranged by size:
417 190 125
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI BanI BanII
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaWI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BstEII BstXI
BstYI BtgI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I MslI MspA1I NcoI NdeI NspI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SapI SbfI ScaI
SfcI SmlI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I
BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI ZraI