Restriction map for
pFN21AE3081
(Linear) (Six-base) MAPSORT of: KIEE3081.seq Check: 2485 from: 1 to: 729
KIEE3081.orf
With 182 enzymes: SgfI *
March 22, 2012 16:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 224 729
Size: 224 505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 729
Size: 322 407
AlwNI CAG_nnn'CTG
Cuts at: 0 126 465 729
Size: 126 339 264
Fragments arranged by size:
339 264 126
ApaI G_GGCC'C
Cuts at: 0 574 729
Size: 574 155
AvaI C'yCGr_G
Cuts at: 0 644 729
Size: 644 85
BanI G'GyrC_C
Cuts at: 0 290 446 729
Size: 290 156 283
Fragments arranged by size:
290 283 156
BanII G_rGCy'C
Cuts at: 0 574 635 721 729
Size: 574 61 86 8
Fragments arranged by size:
574 86 61 8
BbeI G_GCGC'C
Cuts at: 0 294 729
Size: 294 435
BlpI GC'TnA_GC
Cuts at: 0 34 112 729
Size: 34 78 617
Fragments arranged by size:
617 78 34
Bme1580I G_kGCm'C
Cuts at: 0 552 574 686 729
Size: 552 22 112 43
Fragments arranged by size:
552 112 43 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 562 729
Size: 562 167
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 274 729
Size: 274 455
BsaHI Gr'CG_yC
Cuts at: 0 291 729
Size: 291 438
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 508 511 514 523 526 529 532
Size: 505 3 3 3 9 3 3 3
Cuts at: 532 535 538 589 729
Size: 3 3 51 140
Fragments arranged by size:
505 140 51 9 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 107 357 366 593 679 722 729
Size: 107 250 9 227 86 43 7
Fragments arranged by size:
250 227 107 86 43 9 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 729
Size: 604 125
BsiHKAI G_wGCw'C
Cuts at: 0 635 729
Size: 635 94
BsmBI CGTCTCn'nnnn_
Cuts at: 0 319 729
Size: 319 410
Bsp1286I G_dGCh'C
Cuts at: 0 552 574 635 686 721 729
Size: 552 22 61 51 35 8
Fragments arranged by size:
552 61 51 35 22 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 729
Size: 224 505
BstAPI GCAn_nnn'nTGC
Cuts at: 0 126 729
Size: 126 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 423 729
Size: 423 306
EarI CTCTTCn'nnn_
Cuts at: 0 500 729
Size: 500 229
EcoICRI GAG'CTC
Cuts at: 0 633 729
Size: 633 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 729
Size: 322 407
EcoNI CCTnn'n_nnAGG
Cuts at: 0 487 729
Size: 487 242
EcoO109I rG'GnC_Cy
Cuts at: 0 92 104 371 570 729
Size: 92 12 267 199 159
Fragments arranged by size:
267 199 159 92 12
HaeII r_GCGC'y
Cuts at: 0 294 563 729
Size: 294 269 166
Fragments arranged by size:
294 269 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 467 499 729
Size: 467 32 230
Fragments arranged by size:
467 230 32
KasI G'GCGC_C
Cuts at: 0 290 729
Size: 290 439
MspA1I CmG'CkG
Cuts at: 0 111 383 408 444 729
Size: 111 272 25 36 285
Fragments arranged by size:
285 272 111 36 25
NarI GG'CG_CC
Cuts at: 0 291 729
Size: 291 438
PasI CC'CwG_GG
Cuts at: 0 97 246 375 544 729
Size: 97 149 129 169 185
Fragments arranged by size:
185 169 149 129 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 729
Size: 465 264
PfoI T'CCnGG_A
Cuts at: 0 485 729
Size: 485 244
PpuMI rG'GwC_Cy
Cuts at: 0 371 729
Size: 371 358
PspOMI G'GGCC_C
Cuts at: 0 570 729
Size: 570 159
PstI C_TGCA'G
Cuts at: 0 122 443 474 729
Size: 122 321 31 255
Fragments arranged by size:
321 255 122 31
PvuII CAG'CTG
Cuts at: 0 111 408 729
Size: 111 297 321
Fragments arranged by size:
321 297 111
SacI G_AGCT'C
Cuts at: 0 635 729
Size: 635 94
SanDI GG'GwC_CC
Cuts at: 0 371 729
Size: 371 358
SbfI CC_TGCA'GG
Cuts at: 0 122 729
Size: 122 607
SfcI C'TryA_G
Cuts at: 0 118 439 470 729
Size: 118 321 31 259
Fragments arranged by size:
321 259 118 31
SfoI GGC'GCC
Cuts at: 0 292 729
Size: 292 437
StuI AGG'CCT
Cuts at: 0 286 729
Size: 286 443
StyI C'CwwG_G
Cuts at: 0 21 88 626 674 698 729
Size: 21 67 538 48 24 31
Fragments arranged by size:
538 67 48 31 24 21
Enzymes that do cut:
AarI AcuI AlwNI ApaI AvaI BanI BanII BbeI
BlpI Bme1580I BmrI BplI BsaHI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BstAPI BstXI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MspA1I NarI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SanDI SbfI SfcI SfoI StuI StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI