Restriction map for
pFN21AE3074
(Linear) (Six-base) MAPSORT of: KIEE3074.seq Check: 8346 from: 1 to: 702
KIEE3074.orf
With 182 enzymes: SgfI *
March 22, 2012 16:10 ..
AfeI AGC'GCT
Cuts at: 0 387 702
Size: 387 315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 225 702
Size: 225 477
AlwNI CAG_nnn'CTG
Cuts at: 0 615 702
Size: 615 87
AvaI C'yCGr_G
Cuts at: 0 42 60 702
Size: 42 18 642
Fragments arranged by size:
642 42 18
BamHI G'GATC_C
Cuts at: 0 335 702
Size: 335 367
BanI G'GyrC_C
Cuts at: 0 64 702
Size: 64 638
BanII G_rGCy'C
Cuts at: 0 570 702
Size: 570 132
BbeI G_GCGC'C
Cuts at: 0 68 702
Size: 68 634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 702
Size: 154 548
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 702
Size: 211 491
BsaHI Gr'CG_yC
Cuts at: 0 65 296 702
Size: 65 231 406
Fragments arranged by size:
406 231 65
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 702
Size: 28 674
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 535 702
Size: 310 225 167
Fragments arranged by size:
310 225 167
BsiEI CG_ry'CG
Cuts at: 0 165 702
Size: 165 537
BsiHKAI G_wGCw'C
Cuts at: 0 49 550 702
Size: 49 501 152
Fragments arranged by size:
501 152 49
BsmI GAATG_Cn'
Cuts at: 0 202 702
Size: 202 500
Bsp1286I G_dGCh'C
Cuts at: 0 49 550 570 702
Size: 49 501 20 132
Fragments arranged by size:
501 132 49 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 332 680 702
Size: 155 177 348 22
Fragments arranged by size:
348 177 155 22
BsrBI CCG'CTC
Cuts at: 0 8 702
Size: 8 694
BsrFI r'CCGG_y
Cuts at: 0 96 121 702
Size: 96 25 581
Fragments arranged by size:
581 96 25
BssHII G'CGCG_C
Cuts at: 0 155 702
Size: 155 547
BstAPI GCAn_nnn'nTGC
Cuts at: 0 451 702
Size: 451 251
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 702
Size: 588 114
BstYI r'GATC_y
Cuts at: 0 335 605 702
Size: 335 270 97
Fragments arranged by size:
335 270 97
BtgI C'CryG_G
Cuts at: 0 111 702
Size: 111 591
BtsI GCAGTG_nn'
Cuts at: 0 282 500 518 702
Size: 282 218 18 184
Fragments arranged by size:
282 218 184 18
DraI TTT'AAA
Cuts at: 0 428 702
Size: 428 274
EaeI y'GGCC_r
Cuts at: 0 119 162 702
Size: 119 43 540
Fragments arranged by size:
540 119 43
EagI C'GGCC_G
Cuts at: 0 162 702
Size: 162 540
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 702
Size: 526 176
FseI GG_CCGG'CC
Cuts at: 0 125 702
Size: 125 577
HaeII r_GCGC'y
Cuts at: 0 68 111 389 615 702
Size: 68 43 278 226 87
Fragments arranged by size:
278 226 87 68 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 669 701 702
Size: 518 32 119 32 1
Fragments arranged by size:
518 119 32 32 1
HincII GTy'rAC
Cuts at: 0 372 702
Size: 372 330
KasI G'GCGC_C
Cuts at: 0 64 702
Size: 64 638
MslI CAynn'nnrTG
Cuts at: 0 52 702
Size: 52 650
MspA1I CmG'CkG
Cuts at: 0 113 702
Size: 113 589
NaeI GCC'GGC
Cuts at: 0 123 702
Size: 123 579
NarI GG'CG_CC
Cuts at: 0 65 702
Size: 65 637
NdeI CA'TA_TG
Cuts at: 0 489 702
Size: 489 213
NgoMIV G'CCGG_C
Cuts at: 0 121 702
Size: 121 581
NspI r_CATG'y
Cuts at: 0 233 702
Size: 233 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 615 702
Size: 615 87
PshAI GACnn'nnGTC
Cuts at: 0 295 702
Size: 295 407
PspXI vC'TCGA_Gb
Cuts at: 0 42 702
Size: 42 660
PstI C_TGCA'G
Cuts at: 0 277 702
Size: 277 425
SacII CC_GC'GG
Cuts at: 0 114 702
Size: 114 588
SfcI C'TryA_G
Cuts at: 0 273 702
Size: 273 429
SfoI GGC'GCC
Cuts at: 0 66 702
Size: 66 636
SmlI C'TyrA_G
Cuts at: 0 42 169 702
Size: 42 127 533
Fragments arranged by size:
533 127 42
SphI G_CATG'C
Cuts at: 0 233 702
Size: 233 469
StuI AGG'CCT
Cuts at: 0 40 248 702
Size: 40 208 454
Fragments arranged by size:
454 208 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 678 702
Size: 678 24
XhoI C'TCGA_G
Cuts at: 0 42 702
Size: 42 660
XmnI GAAnn'nnTTC
Cuts at: 0 502 702
Size: 502 200
Enzymes that do cut:
AfeI AlfI AlwNI AvaI BamHI BanI BanII BbeI
BpuEI BsaI BsaHI BseRI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI
BtgI BtsI DraI EaeI EagI EciI FseI HaeII
Hin4I HincII KasI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PflMI PshAI PspXI PstI SacII SfcI
SfoI SmlI SphI StuI TaqII XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI
BseYI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StyI SwaI TatI TstI
Tth111I XbaI XcmI XmaI ZraI