Restriction map for
pFN21AE3071
(Linear) (Six-base) MAPSORT of: KIEE3071.seq Check: 8104 from: 1 to: 687
KIEE3071.orf
With 182 enzymes: SgfI *
March 22, 2012 16:10 ..
Acc65I G'GTAC_C
Cuts at: 0 471 687
Size: 471 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 687
Size: 670 17
AflII C'TTAA_G
Cuts at: 0 595 687
Size: 595 92
ApoI r'AATT_y
Cuts at: 0 493 687
Size: 493 194
AseI AT'TA_AT
Cuts at: 0 613 687
Size: 613 74
AvaI C'yCGr_G
Cuts at: 0 25 687
Size: 25 662
BanI G'GyrC_C
Cuts at: 0 471 687
Size: 471 216
BbsI GAAGACnn'nnnn_
Cuts at: 0 324 687
Size: 324 363
BbvCI CC'TCA_GC
Cuts at: 0 5 687
Size: 5 682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 20 54 687
Size: 20 34 633
Fragments arranged by size:
633 34 20
BmgBI CAC'GTC
Cuts at: 0 677 687
Size: 677 10
BmtI G_CTAG'C
Cuts at: 0 412 687
Size: 412 275
Bpu10I CC'TnA_GC
Cuts at: 0 5 687
Size: 5 682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 687
Size: 474 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 687
Size: 1 30 656
Fragments arranged by size:
656 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 687
Size: 22 665
BsiEI CG_ry'CG
Cuts at: 0 180 687
Size: 180 507
BsmI GAATG_Cn'
Cuts at: 0 397 687
Size: 397 290
BsrBI CCG'CTC
Cuts at: 0 89 220 687
Size: 89 131 467
Fragments arranged by size:
467 131 89
BsrDI GCAATG_nn'
Cuts at: 0 677 687
Size: 677 10
BsrGI T'GTAC_A
Cuts at: 0 318 687
Size: 318 369
BstAPI GCAn_nnn'nTGC
Cuts at: 0 140 687
Size: 140 547
BstXI CCAn_nnnn'nTGG
Cuts at: 0 483 687
Size: 483 204
BstYI r'GATC_y
Cuts at: 0 248 687
Size: 248 439
Bsu36I CC'TnA_GG
Cuts at: 0 66 687
Size: 66 621
BtgI C'CryG_G
Cuts at: 0 424 687
Size: 424 263
BtsI GCAGTG_nn'
Cuts at: 0 59 687
Size: 59 628
EaeI y'GGCC_r
Cuts at: 0 165 687
Size: 165 522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 687
Size: 670 17
EcoO109I rG'GnC_Cy
Cuts at: 0 62 687
Size: 62 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 570 602 687
Size: 1 32 537 32 85
Fragments arranged by size:
537 85 32 32 1
HindIII A'AGCT_T
Cuts at: 0 33 592 687
Size: 33 559 95
Fragments arranged by size:
559 95 33
KpnI G_GTAC'C
Cuts at: 0 475 687
Size: 475 212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 687
Size: 417 270
MspA1I CmG'CkG
Cuts at: 0 47 687
Size: 47 640
NheI G'CTAG_C
Cuts at: 0 408 687
Size: 408 279
NspI r_CATG'y
Cuts at: 0 143 325 687
Size: 143 182 362
Fragments arranged by size:
362 182 143
PspXI vC'TCGA_Gb
Cuts at: 0 25 687
Size: 25 662
SfcI C'TryA_G
Cuts at: 0 309 687
Size: 309 378
SmlI C'TyrA_G
Cuts at: 0 25 453 595 687
Size: 25 428 142 92
Fragments arranged by size:
428 142 92 25
StyI C'CwwG_G
Cuts at: 0 581 687
Size: 581 106
TatI w'GTAC_w
Cuts at: 0 318 687
Size: 318 369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 687
Size: 198 489
XhoI C'TCGA_G
Cuts at: 0 25 687
Size: 25 662
Enzymes that do cut:
Acc65I AcuI AflII ApoI AseI AvaI BanI BbsI
BbvCI BcgI BmgBI BmtI Bpu10I BpuEI BsaXI BseRI
BsiEI BsmI BsrBI BsrDI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EaeI Eco57MI EcoO109I Hin4I HindIII
KpnI MmeI MspA1I NheI NspI PspXI SfcI SmlI
StyI TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanII BbeI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
MfeI MluI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XmaI XmnI ZraI