(Linear) (Six-base) MAPSORT of: KIEE3070.seq  Check: 1284  from: 1  to: 681



KIEE3070.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0       1     681 
   Size:          1     680
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     659     681 
   Size:        427     232      22

  Fragments arranged by size: 

                 427     232      22
 
AflIII A'CryG_T

Cuts at:      0     368     681 
   Size:        368     313
 
AlwNI CAG_nnn'CTG

Cuts at:      0      16     681 
   Size:         16     665
 
BanI G'GyrC_C

Cuts at:      0     387     681 
   Size:        387     294
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     540     681 
   Size:        540     141
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     606     681 
   Size:        606      75
 
BfrBI ATG'CAT

Cuts at:      0     662     681 
   Size:        662      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     552     681 
   Size:        552     129
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     681 
   Size:         56     625
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     141     171     368     398     681 
   Size:        141      30     197      30     283

  Fragments arranged by size: 

                 283     197     141      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     392     681 
   Size:        392     289
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     681 
   Size:        125     556
 
BsiHKAI G_wGCw'C

Cuts at:      0     511     681 
   Size:        511     170
 
Bsp1286I G_dGCh'C

Cuts at:      0     511     681 
   Size:        511     170
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       1     681 
   Size:          1     680
 
BsrDI GCAATG_nn'

Cuts at:      0     491     681 
   Size:        491     190
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      16     681 
   Size:         16     665
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     240     681 
   Size:        240     441
 
BstYI r'GATC_y

Cuts at:      0     176     681 
   Size:        176     505
 
BtsI GCAGTG_nn'

Cuts at:      0      47     681 
   Size:         47     634
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0       8      43     681 
   Size:          8      35     638

  Fragments arranged by size: 

                 638      35       8
 
DraI TTT'AAA

Cuts at:      0     213     681 
   Size:        213     468
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     544     681 
   Size:        544     137
 
EarI CTCTTCn'nnn_

Cuts at:      0     297     681 
   Size:        297     384
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     659     681 
   Size:        427     232      22

  Fragments arranged by size: 

                 427     232      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     244     681 
   Size:        244     437
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     236     268     681 
   Size:        236      32     413

  Fragments arranged by size: 

                 413     236      32
 
HindIII A'AGCT_T

Cuts at:      0     185     681 
   Size:        185     496
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     681 
   Size:        279     402
 
MspA1I CmG'CkG

Cuts at:      0     139     681 
   Size:        139     542
 
NsiI A_TGCA'T

Cuts at:      0     664     681 
   Size:        664      17
 
NspI r_CATG'y

Cuts at:      0     372     681 
   Size:        372     309
 
PciI A'CATG_T

Cuts at:      0     368     681 
   Size:        368     313
 
PpuMI rG'GwC_Cy

Cuts at:      0     244     681 
   Size:        244     437
 
PvuII CAG'CTG

Cuts at:      0     139     681 
   Size:        139     542
 
SanDI GG'GwC_CC

Cuts at:      0     244     681 
   Size:        244     437
 
SmlI C'TyrA_G

Cuts at:      0      71     681 
   Size:         71     610
 
SspI AAT'ATT

Cuts at:      0     361     681 
   Size:        361     320
 
StyI C'CwwG_G

Cuts at:      0     320     681 
   Size:        320     361
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     368     400     681 
   Size:        368      32     281

  Fragments arranged by size: 

                 368     281      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     681 
   Size:        255     426
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     BanI     BbsI    BciVI    BfrBI
     BmrI    BpuEI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI   BstAPI    BstXI    BstYI     BtsI    CspCI     DraI     DrdI
     EarI  Eco57MI EcoO109I    Hin4I  HindIII     MmeI   MspA1I     NsiI
     NspI     PciI    PpuMI    PvuII    SanDI     SmlI     SspI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI