Restriction map for
pFN21AE3068
(Linear) (Six-base) MAPSORT of: KIEE3068.seq Check: 5865 from: 1 to: 672
KIEE3068.orf
With 182 enzymes: SgfI *
March 22, 2012 16:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 672
Size: 69 603
AflIII A'CryG_T
Cuts at: 0 359 672
Size: 359 313
ApaI G_GGCC'C
Cuts at: 0 238 672
Size: 238 434
ApaLI G'TGCA_C
Cuts at: 0 416 672
Size: 416 256
ApoI r'AATT_y
Cuts at: 0 391 404 672
Size: 391 13 268
Fragments arranged by size:
391 268 13
BanII G_rGCy'C
Cuts at: 0 238 672
Size: 238 434
BbsI GAAGACnn'nnnn_
Cuts at: 0 260 531 672
Size: 260 271 141
Fragments arranged by size:
271 260 141
BclI T'GATC_A
Cuts at: 0 263 672
Size: 263 409
BglI GCCn_nnn'nGGC
Cuts at: 0 114 672
Size: 114 558
Bme1580I G_kGCm'C
Cuts at: 0 238 420 672
Size: 238 182 252
Fragments arranged by size:
252 238 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 526 672
Size: 526 146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 658 672
Size: 389 269 14
Fragments arranged by size:
389 269 14
BseYI C'CCAG_C
Cuts at: 0 183 339 533 672
Size: 183 156 194 139
Fragments arranged by size:
194 183 156 139
BsiHKAI G_wGCw'C
Cuts at: 0 420 672
Size: 420 252
Bsp1286I G_dGCh'C
Cuts at: 0 238 420 672
Size: 238 182 252
Fragments arranged by size:
252 238 182
BspHI T'CATG_A
Cuts at: 0 266 672
Size: 266 406
BstBI TT'CG_AA
Cuts at: 0 69 672
Size: 69 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 672
Size: 231 441
BstYI r'GATC_y
Cuts at: 0 140 672
Size: 140 532
Bsu36I CC'TnA_GG
Cuts at: 0 559 672
Size: 559 113
BtgI C'CryG_G
Cuts at: 0 326 672
Size: 326 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 291 672
Size: 291 381
BtsI GCAGTG_nn'
Cuts at: 0 219 672
Size: 219 453
DraI TTT'AAA
Cuts at: 0 204 672
Size: 204 468
DraIII CAC_nnn'GTG
Cuts at: 0 387 672
Size: 387 285
DrdI GACnn_nn'nnGTC
Cuts at: 0 535 672
Size: 535 137
EaeI y'GGCC_r
Cuts at: 0 625 672
Size: 625 47
EarI CTCTTCn'nnn_
Cuts at: 0 487 672
Size: 487 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 389 658 672
Size: 69 320 269 14
Fragments arranged by size:
320 269 69 14
EcoO109I rG'GnC_Cy
Cuts at: 0 235 672
Size: 235 437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 349 381 672
Size: 255 32 62 32 291
Fragments arranged by size:
291 255 62 32 32
MscI TGG'CCA
Cuts at: 0 627 672
Size: 627 45
MslI CAynn'nnrTG
Cuts at: 0 265 672
Size: 265 407
MspA1I CmG'CkG
Cuts at: 0 533 672
Size: 533 139
NspI r_CATG'y
Cuts at: 0 363 657 672
Size: 363 294 15
Fragments arranged by size:
363 294 15
PciI A'CATG_T
Cuts at: 0 359 672
Size: 359 313
PfoI T'CCnGG_A
Cuts at: 0 524 672
Size: 524 148
PspOMI G'GGCC_C
Cuts at: 0 234 672
Size: 234 438
PstI C_TGCA'G
Cuts at: 0 214 672
Size: 214 458
PvuII CAG'CTG
Cuts at: 0 533 672
Size: 533 139
SfcI C'TryA_G
Cuts at: 0 210 672
Size: 210 462
SphI G_CATG'C
Cuts at: 0 657 672
Size: 657 15
StuI AGG'CCT
Cuts at: 0 299 672
Size: 299 373
StyI C'CwwG_G
Cuts at: 0 134 672
Size: 134 538
TatI w'GTAC_w
Cuts at: 0 155 362 672
Size: 155 207 310
Fragments arranged by size:
310 207 155
XmnI GAAnn'nnTTC
Cuts at: 0 481 672
Size: 481 191
Enzymes that do cut:
AcuI AflIII ApaI ApaLI ApoI BanII BbsI BclI
BglI Bme1580I BmrI BpmI BseYI BsiHKAI Bsp1286I BspHI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EarI Eco57MI EcoO109I Hin4I MscI
MslI MspA1I NspI PciI PfoI PspOMI PstI PvuII
SfcI SphI StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I
ClaI CspCI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI