Restriction map for
pFN21AE3064
(Linear) (Six-base) MAPSORT of: KIEE3064.seq Check: 4576 from: 1 to: 657
KIEE3064.orf
With 182 enzymes: SgfI *
March 22, 2012 16:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 657
Size: 282 375
AfeI AGC'GCT
Cuts at: 0 535 657
Size: 535 122
AleI CACnn'nnGTG
Cuts at: 0 283 657
Size: 283 374
ApaLI G'TGCA_C
Cuts at: 0 178 418 657
Size: 178 240 239
Fragments arranged by size:
240 239 178
AvaI C'yCGr_G
Cuts at: 0 54 657
Size: 54 603
BanI G'GyrC_C
Cuts at: 0 109 441 625 657
Size: 109 332 184 32
Fragments arranged by size:
332 184 109 32
BanII G_rGCy'C
Cuts at: 0 143 368 523 657
Size: 143 225 155 134
Fragments arranged by size:
225 155 143 134
BbeI G_GCGC'C
Cuts at: 0 445 657
Size: 445 212
BbsI GAAGACnn'nnnn_
Cuts at: 0 288 657
Size: 288 369
Bme1580I G_kGCm'C
Cuts at: 0 150 182 422 657
Size: 150 32 240 235
Fragments arranged by size:
240 235 150 32
BmgBI CAC'GTC
Cuts at: 0 285 657
Size: 285 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 379 657
Size: 172 207 278
Fragments arranged by size:
278 207 172
Bpu10I CC'TnA_GC
Cuts at: 0 137 657
Size: 137 520
BsaHI Gr'CG_yC
Cuts at: 0 40 188 442 657
Size: 40 148 254 215
Fragments arranged by size:
254 215 148 40
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 376 415 657
Size: 169 207 39 242
Fragments arranged by size:
242 207 169 39
BsiHKAI G_wGCw'C
Cuts at: 0 143 182 368 422 657
Size: 143 39 186 54 235
Fragments arranged by size:
235 186 143 54 39
Bsp1286I G_dGCh'C
Cuts at: 0 143 150 182 368 422 523 657
Size: 143 7 32 186 54 101 134
Fragments arranged by size:
186 143 134 101 54 32 7
BsrFI r'CCGG_y
Cuts at: 0 112 657
Size: 112 545
BstAPI GCAn_nnn'nTGC
Cuts at: 0 245 657
Size: 245 412
EaeI y'GGCC_r
Cuts at: 0 570 657
Size: 570 87
EcoICRI GAG'CTC
Cuts at: 0 141 366 657
Size: 141 225 291
Fragments arranged by size:
291 225 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 282 379 657
Size: 172 110 97 278
Fragments arranged by size:
278 172 110 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 657
Size: 154 503
EcoO109I rG'GnC_Cy
Cuts at: 0 60 657
Size: 60 597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 552 657
Size: 552 105
HaeII r_GCGC'y
Cuts at: 0 171 358 445 537 657
Size: 171 187 87 92 120
Fragments arranged by size:
187 171 120 92 87
KasI G'GCGC_C
Cuts at: 0 441 657
Size: 441 216
MslI CAynn'nnrTG
Cuts at: 0 283 657
Size: 283 374
NarI GG'CG_CC
Cuts at: 0 442 657
Size: 442 215
NruI TCG'CGA
Cuts at: 0 413 657
Size: 413 244
PfoI T'CCnGG_A
Cuts at: 0 347 657
Size: 347 310
PpuMI rG'GwC_Cy
Cuts at: 0 60 657
Size: 60 597
SacI G_AGCT'C
Cuts at: 0 143 368 657
Size: 143 225 289
Fragments arranged by size:
289 225 143
SanDI GG'GwC_CC
Cuts at: 0 60 657
Size: 60 597
SexAI A'CCwGG_T
Cuts at: 0 581 657
Size: 581 76
SfoI GGC'GCC
Cuts at: 0 443 657
Size: 443 214
SmaI CCC'GGG
Cuts at: 0 56 657
Size: 56 601
StuI AGG'CCT
Cuts at: 0 160 657
Size: 160 497
Tth111I GACn'n_nGTC
Cuts at: 0 292 657
Size: 292 365
XmaI C'CCGG_G
Cuts at: 0 54 657
Size: 54 603
XmnI GAAnn'nnTTC
Cuts at: 0 379 657
Size: 379 278
Enzymes that do cut:
AcuI AfeI AleI ApaLI AvaI BanI BanII BbeI
BbsI Bme1580I BmgBI BpmI Bpu10I BsaHI BseRI BsiHKAI
Bsp1286I BsrFI BstAPI EaeI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII KasI MslI NarI NruI PfoI PpuMI
SacI SanDI SexAI SfoI SmaI StuI Tth111I XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI TstI XbaI
XcmI XhoI ZraI