Restriction map for
pFN21AE3063
(Linear) (Six-base) MAPSORT of: KIEE3063.seq Check: 2850 from: 1 to: 657
KIEE3063.orf
With 182 enzymes: SgfI *
March 22, 2012 16:08 ..
AccI GT'mk_AC
Cuts at: 0 191 657
Size: 191 466
AfeI AGC'GCT
Cuts at: 0 247 657
Size: 247 410
AflIII A'CryG_T
Cuts at: 0 426 657
Size: 426 231
AlwNI CAG_nnn'CTG
Cuts at: 0 647 657
Size: 647 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 657
Size: 516 141
BbvCI CC'TCA_GC
Cuts at: 0 440 657
Size: 440 217
BglII A'GATC_T
Cuts at: 0 221 657
Size: 221 436
BmrI ACTGGGnnnn_n'
Cuts at: 0 354 657
Size: 354 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 436 567 611 657
Size: 32 404 131 44 46
Fragments arranged by size:
404 131 46 44 32
Bpu10I CC'TnA_GC
Cuts at: 0 440 588 657
Size: 440 148 69
Fragments arranged by size:
440 148 69
BseYI C'CCAG_C
Cuts at: 0 577 635 640 657
Size: 577 58 5 17
Fragments arranged by size:
577 58 17 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 657
Size: 358 299
BsiEI CG_ry'CG
Cuts at: 0 614 657
Size: 614 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 510 657
Size: 510 147
BstYI r'GATC_y
Cuts at: 0 221 657
Size: 221 436
BtgI C'CryG_G
Cuts at: 0 31 263 284 614 657
Size: 31 232 21 330 43
Fragments arranged by size:
330 232 43 31 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 294 657
Size: 294 363
BtsI GCAGTG_nn'
Cuts at: 0 99 657
Size: 99 558
ClaI AT'CG_AT
Cuts at: 0 170 657
Size: 170 487
EaeI y'GGCC_r
Cuts at: 0 447 611 657
Size: 447 164 46
Fragments arranged by size:
447 164 46
EagI C'GGCC_G
Cuts at: 0 611 657
Size: 611 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 436 567 611 657
Size: 32 404 131 44 46
Fragments arranged by size:
404 131 46 44 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 126 540 657
Size: 126 414 117
Fragments arranged by size:
414 126 117
HaeII r_GCGC'y
Cuts at: 0 249 534 657
Size: 249 285 123
Fragments arranged by size:
285 249 123
MslI CAynn'nnrTG
Cuts at: 0 394 511 657
Size: 394 117 146
Fragments arranged by size:
394 146 117
MspA1I CmG'CkG
Cuts at: 0 10 235 334 647 653 657
Size: 10 225 99 313 6 4
Fragments arranged by size:
313 225 99 10 6 4
NcoI C'CATG_G
Cuts at: 0 284 657
Size: 284 373
PstI C_TGCA'G
Cuts at: 0 221 652 657
Size: 221 431 5
Fragments arranged by size:
431 221 5
PvuII CAG'CTG
Cuts at: 0 10 647 653 657
Size: 10 637 6 4
Fragments arranged by size:
637 10 6 4
ScaI AGT'ACT
Cuts at: 0 159 657
Size: 159 498
SfcI C'TryA_G
Cuts at: 0 217 648 657
Size: 217 431 9
Fragments arranged by size:
431 217 9
StyI C'CwwG_G
Cuts at: 0 284 468 497 657
Size: 284 184 29 160
Fragments arranged by size:
284 184 160 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 657
Size: 34 623
TatI w'GTAC_w
Cuts at: 0 157 657
Size: 157 500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 393 425 657
Size: 393 32 232
Fragments arranged by size:
393 232 32
Enzymes that do cut:
AccI AfeI AflIII AlwNI BbsI BbvCI BglII BmrI
BpmI Bpu10I BseYI BsgI BsiEI BspMI BstYI BtgI
BtgZI BtsI ClaI EaeI EagI Eco57MI FalI HaeII
MslI MspA1I NcoI PstI PvuII ScaI SfcI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I CspCI DraI DraIII
DrdI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI