(Linear) (Six-base) MAPSORT of: KIEE3061.seq  Check: 5039  from: 1  to: 648



KIEE3061.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     648 
   Size:        128     520
 
AhdI GACnn_n'nnGTC

Cuts at:      0      30     648 
   Size:         30     618
 
BamHI G'GATC_C

Cuts at:      0     243     648 
   Size:        243     405
 
BanI G'GyrC_C

Cuts at:      0     126     648 
   Size:        126     522
 
BlpI GC'TnA_GC

Cuts at:      0     454     648 
   Size:        454     194
 
Bme1580I G_kGCm'C

Cuts at:      0     131     648 
   Size:        131     517
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     648 
   Size:        166     482
 
BseYI C'CCAG_C

Cuts at:      0     391     648 
   Size:        391     257
 
BsiHKAI G_wGCw'C

Cuts at:      0     432     648 
   Size:        432     216
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     432     648 
   Size:        131     301     216

  Fragments arranged by size: 

                 301     216     131
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     153     648 
   Size:        153     495
 
BsrFI r'CCGG_y

Cuts at:      0     395     648 
   Size:        395     253
 
BstYI r'GATC_y

Cuts at:      0     243     503     648 
   Size:        243     260     145

  Fragments arranged by size: 

                 260     243     145
 
BtsI GCAGTG_nn'

Cuts at:      0     430     648 
   Size:        430     218
 
DraI TTT'AAA

Cuts at:      0     179     206     648 
   Size:        179      27     442

  Fragments arranged by size: 

                 442     179      27
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     107     648 
   Size:         86      21     541

  Fragments arranged by size: 

                 541      86      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     648 
   Size:        128     520
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4     648 
   Size:          4     644
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     648 
   Size:         16      32     600

  Fragments arranged by size: 

                 600      32      16
 
HincII GTy'rAC

Cuts at:      0     624     648 
   Size:        624      24
 
HpaI GTT'AAC

Cuts at:      0     624     648 
   Size:        624      24
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     648 
   Size:        221     427
 
NaeI GCC'GGC

Cuts at:      0     397     648 
   Size:        397     251
 
NgoMIV G'CCGG_C

Cuts at:      0     395     648 
   Size:        395     253
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     194     648 
   Size:        194     454
 
PpuMI rG'GwC_Cy

Cuts at:      0       4     648 
   Size:          4     644
 
PsiI TTA'TAA

Cuts at:      0     385     648 
   Size:        385     263
 
SmlI C'TyrA_G

Cuts at:      0     145     648 
   Size:        145     503
 
SspI AAT'ATT

Cuts at:      0      63     184     648 
   Size:         63     121     464

  Fragments arranged by size: 

                 464     121      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     515     648 
   Size:        515     133
 
XbaI T'CTAG_A

Cuts at:      0     403     648 
   Size:        403     245
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     332     648 
   Size:        332     316
 
XmnI GAAnn'nnTTC

Cuts at:      0     326     648 
   Size:        326     322
 

 Enzymes that do cut:

     AcuI     AhdI    BamHI     BanI     BlpI Bme1580I    BpuEI    BseYI
  BsiHKAI Bsp1286I    BspMI    BsrFI    BstYI     BtsI     DraI     EarI
  Eco57MI EcoO109I    Hin4I   HincII     HpaI     MmeI     NaeI   NgoMIV
    PflMI    PpuMI     PsiI     SmlI     SspI    TaqII     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XhoI     XmaI     ZraI