Restriction map for
pFN21AE3057
(Linear) (Six-base) MAPSORT of: KIEE3057.seq Check: 8576 from: 1 to: 636
KIEE3057.orf
With 182 enzymes: SgfI *
March 22, 2012 16:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 215 636
Size: 215 421
AfeI AGC'GCT
Cuts at: 0 86 448 636
Size: 86 362 188
Fragments arranged by size:
362 188 86
AgeI A'CCGG_T
Cuts at: 0 316 636
Size: 316 320
ApaLI G'TGCA_C
Cuts at: 0 202 636
Size: 202 434
ApoI r'AATT_y
Cuts at: 0 330 636
Size: 330 306
BanI G'GyrC_C
Cuts at: 0 319 636
Size: 319 317
BanII G_rGCy'C
Cuts at: 0 21 636
Size: 21 615
Bme1580I G_kGCm'C
Cuts at: 0 206 636
Size: 206 430
BmgBI CAC'GTC
Cuts at: 0 186 363 636
Size: 186 177 273
Fragments arranged by size:
273 186 177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 636
Size: 213 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 382 636
Size: 52 330 254
Fragments arranged by size:
330 254 52
BsaHI Gr'CG_yC
Cuts at: 0 590 636
Size: 590 46
BsaWI w'CCGG_w
Cuts at: 0 119 316 482 636
Size: 119 197 166 154
Fragments arranged by size:
197 166 154 119
BseYI C'CCAG_C
Cuts at: 0 227 618 636
Size: 227 391 18
Fragments arranged by size:
391 227 18
BsiHKAI G_wGCw'C
Cuts at: 0 61 206 581 636
Size: 61 145 375 55
Fragments arranged by size:
375 145 61 55
Bsp1286I G_dGCh'C
Cuts at: 0 21 61 206 581 636
Size: 21 40 145 375 55
Fragments arranged by size:
375 145 55 40 21
BspHI T'CATG_A
Cuts at: 0 500 636
Size: 500 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 636
Size: 215 421
BsrBI CCG'CTC
Cuts at: 0 474 636
Size: 474 162
BsrFI r'CCGG_y
Cuts at: 0 316 636
Size: 316 320
BtgI C'CryG_G
Cuts at: 0 95 636
Size: 95 541
BtsI GCAGTG_nn'
Cuts at: 0 599 636
Size: 599 37
DraIII CAC_nnn'GTG
Cuts at: 0 201 636
Size: 201 435
EaeI y'GGCC_r
Cuts at: 0 438 636
Size: 438 198
EarI CTCTTCn'nnn_
Cuts at: 0 49 636
Size: 49 587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 427 636
Size: 427 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 382 636
Size: 52 330 254
Fragments arranged by size:
330 254 52
HaeII r_GCGC'y
Cuts at: 0 88 450 481 636
Size: 88 362 31 155
Fragments arranged by size:
362 155 88 31
MscI TGG'CCA
Cuts at: 0 440 636
Size: 440 196
MslI CAynn'nnrTG
Cuts at: 0 574 636
Size: 574 62
MspA1I CmG'CkG
Cuts at: 0 97 408 618 636
Size: 97 311 210 18
Fragments arranged by size:
311 210 97 18
PacI TTA_AT'TAA
Cuts at: 0 248 636
Size: 248 388
PfoI T'CCnGG_A
Cuts at: 0 125 467 636
Size: 125 342 169
Fragments arranged by size:
342 169 125
PshAI GACnn'nnGTC
Cuts at: 0 277 636
Size: 277 359
PvuII CAG'CTG
Cuts at: 0 408 636
Size: 408 228
SacII CC_GC'GG
Cuts at: 0 98 636
Size: 98 538
SapI GCTCTTCn'nnn_
Cuts at: 0 49 636
Size: 49 587
SexAI A'CCwGG_T
Cuts at: 0 383 636
Size: 383 253
SgrAI Cr'CCGG_yG
Cuts at: 0 316 636
Size: 316 320
StyI C'CwwG_G
Cuts at: 0 323 636
Size: 323 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 636
Size: 73 32 531
Fragments arranged by size:
531 73 32
Tth111I GACn'n_nGTC
Cuts at: 0 385 636
Size: 385 251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 636
Size: 381 255
Enzymes that do cut:
AarI AfeI AgeI ApaLI ApoI BanI BanII Bme1580I
BmgBI BplI BpmI BsaHI BsaWI BseYI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BtgI BtsI DraIII EaeI
EarI EciI Eco57MI HaeII MscI MslI MspA1I PacI
PfoI PshAI PvuII SacII SapI SexAI SgrAI StyI
TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI XbaI XhoI XmaI XmnI
ZraI