(Linear) (Six-base) MAPSORT of: KIEE3051.seq  Check: 800  from: 1  to: 579



KIEE3051.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

ApoI r'AATT_y

Cuts at:      0     152     579 
   Size:        152     427
 
BamHI G'GATC_C

Cuts at:      0     262     579 
   Size:        262     317
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      41     579 
   Size:         41     538
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     579 
   Size:        242     337
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      24      27     121     579 
   Size:         24       3      94     458

  Fragments arranged by size: 

                 458      94      24       3
 
BstYI r'GATC_y

Cuts at:      0     262     579 
   Size:        262     317
 
BtsI GCAGTG_nn'

Cuts at:      0     207     579 
   Size:        207     372
 
EaeI y'GGCC_r

Cuts at:      0     548     579 
   Size:        548      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     184     579 
   Size:        184     395
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     579 
   Size:        242     337
 
EcoO109I rG'GnC_Cy

Cuts at:      0     253     579 
   Size:        253     326
 
EcoRI G'AATT_C

Cuts at:      0     152     579 
   Size:        152     427
 
EcoRV GAT'ATC

Cuts at:      0     297     579 
   Size:        297     282
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     463     579 
   Size:        463     116
 
HaeII r_GCGC'y

Cuts at:      0     146     579 
   Size:        146     433
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     579 
   Size:         77     502
 
MscI TGG'CCA

Cuts at:      0     550     579 
   Size:        550      29
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     557     579 
   Size:        557      22
 
PfoI T'CCnGG_A

Cuts at:      0     258     579 
   Size:        258     321
 
SspI AAT'ATT

Cuts at:      0     369     579 
   Size:        369     210
 
StyI C'CwwG_G

Cuts at:      0     491     579 
   Size:        491      88
 

 Enzymes that do cut:

     ApoI    BamHI     BbsI     BpmI    BseRI    BstYI     BtsI     EaeI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    HaeII     MmeI
     MscI    PflMI     PfoI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI
    EcoNI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI