(Linear) (Six-base) MAPSORT of: KIEE3050.seq  Check: 5652  from: 1  to: 567



KIEE3050.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     293     325     567 
   Size:        293      32     242

  Fragments arranged by size: 

                 293     242      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
ApoI r'AATT_y

Cuts at:      0     319     567 
   Size:        319     248
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     223     567 
   Size:        223     344
 
BglII A'GATC_T

Cuts at:      0     517     567 
   Size:        517      50
 
BlpI GC'TnA_GC

Cuts at:      0      40     567 
   Size:         40     527
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     567 
   Size:        206     361
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     293     323     567 
   Size:        293      30     244

  Fragments arranged by size: 

                 293     244      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     567 
   Size:        205     362
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     567 
   Size:         41     526
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     567 
   Size:         41     526
 
BspHI T'CATG_A

Cuts at:      0     512     567 
   Size:        512      55
 
BstYI r'GATC_y

Cuts at:      0     517     567 
   Size:        517      50
 
EaeI y'GGCC_r

Cuts at:      0      26     567 
   Size:         26     541
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     383     567 
   Size:        274     109     184

  Fragments arranged by size: 

                 274     184     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     567 
   Size:        107     460
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     271     281     303     494     526     567 
   Size:        249      22      10      22     191      32      41

  Fragments arranged by size: 

                 249     191      41      32      22      22      10
 
NspI r_CATG'y

Cuts at:      0     365     567 
   Size:        365     202
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      93     567 
   Size:         93     474
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     293     325     567 
   Size:        293      32     242

  Fragments arranged by size: 

                 293     242      32
 
SmlI C'TyrA_G

Cuts at:      0     221     567 
   Size:        221     346
 
SspI AAT'ATT

Cuts at:      0     551     567 
   Size:        551      16
 
XmnI GAAnn'nnTTC

Cuts at:      0     436     567 
   Size:        436     131
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI     BbsI    BglII     BlpI    BpuEI
    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BstYI     EaeI     EarI
  Eco57MI EcoO109I    Hin4I     NspI    PflMI     PpiI     SmlI     SspI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI