(Linear) (Six-base) MAPSORT of: KIEE3049.seq  Check: 5104  from: 1  to: 567



KIEE3049.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
ApoI r'AATT_y

Cuts at:      0     178     193     319     567 
   Size:        178      15     126     248

  Fragments arranged by size: 

                 248     178     126      15
 
BlpI GC'TnA_GC

Cuts at:      0      40     567 
   Size:         40     527
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     158     567 
   Size:        158     409
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     567 
   Size:        206     361
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     298     567 
   Size:        298     269
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     147     567 
   Size:        147     420
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     567 
   Size:         41     526
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     567 
   Size:         41     526
 
BspHI T'CATG_A

Cuts at:      0     512     567 
   Size:        512      55
 
BsrDI GCAATG_nn'

Cuts at:      0       4     567 
   Size:          4     563
 
BstYI r'GATC_y

Cuts at:      0     517     567 
   Size:        517      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     383     567 
   Size:        274     109     184

  Fragments arranged by size: 

                 274     184     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     453     567 
   Size:        453     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     494     526     567 
   Size:        249      32     213      32      41

  Fragments arranged by size: 

                 249     213      41      32      32
 
MspA1I CmG'CkG

Cuts at:      0      44     567 
   Size:         44     523
 
PvuII CAG'CTG

Cuts at:      0      44     567 
   Size:         44     523
 
SmlI C'TyrA_G

Cuts at:      0     221     567 
   Size:        221     346
 
XmnI GAAnn'nnTTC

Cuts at:      0     436     567 
   Size:        436     131
 

 Enzymes that do cut:

     AcuI     ApoI     BlpI     BplI    BpuEI     BsaI    BseRI  BsiHKAI
 Bsp1286I    BspHI    BsrDI    BstYI     EarI  Eco57MI     FalI    Hin4I
   MspA1I    PvuII     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI