(Linear) (Six-base) MAPSORT of: KIEE3048.seq  Check: 6779  from: 1  to: 567



KIEE3048.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     567 
   Size:        255     312
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     293     325     567 
   Size:        293      32     242

  Fragments arranged by size: 

                 293     242      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
ApoI r'AATT_y

Cuts at:      0     172     319     567 
   Size:        172     147     248

  Fragments arranged by size: 

                 248     172     147
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     223     567 
   Size:        223     344
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     567 
   Size:        206     361
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     293     323     567 
   Size:        293      30     244

  Fragments arranged by size: 

                 293     244      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     205     567 
   Size:        202       3     362

  Fragments arranged by size: 

                 362     202       3
 
BspHI T'CATG_A

Cuts at:      0     512     567 
   Size:        512      55
 
BstYI r'GATC_y

Cuts at:      0     517     567 
   Size:        517      50
 
EaeI y'GGCC_r

Cuts at:      0     201     567 
   Size:        201     366
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     383     567 
   Size:        274     109     184

  Fragments arranged by size: 

                 274     184     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     567 
   Size:        255     312
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     567 
   Size:        107     460
 
EcoRI G'AATT_C

Cuts at:      0     172     567 
   Size:        172     395
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     271     281     303     494     526     567 
   Size:        249      22      10      22     191      32      41

  Fragments arranged by size: 

                 249     191      41      32      22      22      10
 
MscI TGG'CCA

Cuts at:      0     203     567 
   Size:        203     364
 
NspI r_CATG'y

Cuts at:      0     365     567 
   Size:        365     202
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     293     325     567 
   Size:        293      32     242

  Fragments arranged by size: 

                 293     242      32
 
SmlI C'TyrA_G

Cuts at:      0     221     567 
   Size:        221     346
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60     567 
   Size:         60     507
 
XmnI GAAnn'nnTTC

Cuts at:      0     436     567 
   Size:        436     131
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI     BbsI    BpuEI    BsaXI    BseRI
    BspHI    BstYI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I
     MscI     NspI     PpiI     SmlI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI