(Linear) (Six-base) MAPSORT of: KIEE3042.seq  Check: 8010  from: 1  to: 546



KIEE3042.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:21  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     193     546 
   Size:        193     353
 
ApoI r'AATT_y

Cuts at:      0     466     525     546 
   Size:        466      59      21

  Fragments arranged by size: 

                 466      59      21
 
AvaI C'yCGr_G

Cuts at:      0      34      73     546 
   Size:         34      39     473

  Fragments arranged by size: 

                 473      39      34
 
BanII G_rGCy'C

Cuts at:      0      80     546 
   Size:         80     466
 
BbvCI CC'TCA_GC

Cuts at:      0     186     546 
   Size:        186     360
 
BmgBI CAC'GTC

Cuts at:      0     459     546 
   Size:        459      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     546 
   Size:        238     308
 
Bpu10I CC'TnA_GC

Cuts at:      0     186     546 
   Size:        186     360
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154     490     546 
   Size:        154     336      56

  Fragments arranged by size: 

                 336     154      56
 
BsaHI Gr'CG_yC

Cuts at:      0     181     546 
   Size:        181     365
 
BseYI C'CCAG_C

Cuts at:      0     394     546 
   Size:        394     152
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     546 
   Size:         80     466
 
BsrFI r'CCGG_y

Cuts at:      0      43      93     546 
   Size:         43      50     453

  Fragments arranged by size: 

                 453      50      43
 
BtgI C'CryG_G

Cuts at:      0      65     323     546 
   Size:         65     258     223

  Fragments arranged by size: 

                 258     223      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     345     546 
   Size:        345     201
 
EaeI y'GGCC_r

Cuts at:      0     137     356     461     546 
   Size:        137     219     105      85

  Fragments arranged by size: 

                 219     137     105      85
 
EarI CTCTTCn'nnn_

Cuts at:      0     515     546 
   Size:        515      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     546 
   Size:        238     308
 
EcoRI G'AATT_C

Cuts at:      0     525     546 
   Size:        525      21
 
HaeII r_GCGC'y

Cuts at:      0     211     546 
   Size:        211     335
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     523     546 
   Size:        523      23
 
MscI TGG'CCA

Cuts at:      0     463     546 
   Size:        463      83
 
MspA1I CmG'CkG

Cuts at:      0      67     264     325     394     546 
   Size:         67     197      61      69     152

  Fragments arranged by size: 

                 197     152      69      67      61
 
NaeI GCC'GGC

Cuts at:      0      45      95     546 
   Size:         45      50     451

  Fragments arranged by size: 

                 451      50      45
 
NgoMIV G'CCGG_C

Cuts at:      0      43      93     546 
   Size:         43      50     453

  Fragments arranged by size: 

                 453      50      43
 
PstI C_TGCA'G

Cuts at:      0     492     546 
   Size:        492      54
 
PvuII CAG'CTG

Cuts at:      0     264     546 
   Size:        264     282
 
SacII CC_GC'GG

Cuts at:      0      68     326     546 
   Size:         68     258     220

  Fragments arranged by size: 

                 258     220      68
 
ScaI AGT'ACT

Cuts at:      0     370     546 
   Size:        370     176
 
SfcI C'TryA_G

Cuts at:      0     488     546 
   Size:        488      58
 
SmaI CCC'GGG

Cuts at:      0      75     546 
   Size:         75     471
 
TatI w'GTAC_w

Cuts at:      0     236     368     546 
   Size:        236     132     178

  Fragments arranged by size: 

                 236     178     132
 
XmaI C'CCGG_G

Cuts at:      0      73     546 
   Size:         73     473
 

 Enzymes that do cut:

    AlwNI     ApoI     AvaI    BanII    BbvCI    BmgBI     BpmI   Bpu10I
     BsaI    BsaHI    BseYI Bsp1286I    BsrFI     BtgI    BtgZI     EaeI
     EarI  Eco57MI    EcoRI    HaeII    Hin4I     MscI   MspA1I     NaeI
   NgoMIV     PstI    PvuII    SacII     ScaI     SfcI     SmaI     TatI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI