Restriction map for
pFN21AE3040
(Linear) (Six-base) MAPSORT of: KIEE3040.seq Check: 6679 from: 1 to: 531
KIEE3040.orf
With 182 enzymes: SgfI *
July 3, 2012 17:20 ..
AccI GT'mk_AC
Cuts at: 0 13 531
Size: 13 518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 531
Size: 259 272
ApoI r'AATT_y
Cuts at: 0 190 531
Size: 190 341
AvaI C'yCGr_G
Cuts at: 0 346 531
Size: 346 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 334 531
Size: 334 197
BglII A'GATC_T
Cuts at: 0 210 531
Size: 210 321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 361 527 531
Size: 361 166 4
Fragments arranged by size:
361 166 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 202 531
Size: 39 163 329
Fragments arranged by size:
329 163 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 531
Size: 346 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 531
Size: 470 61
BsiHKAI G_wGCw'C
Cuts at: 0 513 531
Size: 513 18
Bsp1286I G_dGCh'C
Cuts at: 0 513 531
Size: 513 18
BsrDI GCAATG_nn'
Cuts at: 0 98 531
Size: 98 433
BsrFI r'CCGG_y
Cuts at: 0 259 288 531
Size: 259 29 243
Fragments arranged by size:
259 243 29
BssSI C'ACGA_G
Cuts at: 0 501 531
Size: 501 30
BstYI r'GATC_y
Cuts at: 0 210 521 531
Size: 210 311 10
Fragments arranged by size:
311 210 10
Bsu36I CC'TnA_GG
Cuts at: 0 482 531
Size: 482 49
BtgI C'CryG_G
Cuts at: 0 74 299 387 531
Size: 74 225 88 144
Fragments arranged by size:
225 144 88 74
BtsI GCAGTG_nn'
Cuts at: 0 438 531
Size: 438 93
EaeI y'GGCC_r
Cuts at: 0 77 531
Size: 77 454
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 531
Size: 163 368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 531
Size: 259 272
EcoO109I rG'GnC_Cy
Cuts at: 0 43 531
Size: 43 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 531
Size: 131 32 368
Fragments arranged by size:
368 131 32
HincII GTy'rAC
Cuts at: 0 234 531
Size: 234 297
HindIII A'AGCT_T
Cuts at: 0 200 531
Size: 200 331
MscI TGG'CCA
Cuts at: 0 79 531
Size: 79 452
MslI CAynn'nnrTG
Cuts at: 0 431 531
Size: 431 100
MspA1I CmG'CkG
Cuts at: 0 126 531
Size: 126 405
NaeI GCC'GGC
Cuts at: 0 261 531
Size: 261 270
NcoI C'CATG_G
Cuts at: 0 74 299 531
Size: 74 225 232
Fragments arranged by size:
232 225 74
NgoMIV G'CCGG_C
Cuts at: 0 259 531
Size: 259 272
NspI r_CATG'y
Cuts at: 0 279 531
Size: 279 252
PflMI CCAn_nnn'nTGG
Cuts at: 0 305 531
Size: 305 226
PstI C_TGCA'G
Cuts at: 0 366 531
Size: 366 165
SfcI C'TryA_G
Cuts at: 0 362 531
Size: 362 169
SmlI C'TyrA_G
Cuts at: 0 54 217 531
Size: 54 163 314
Fragments arranged by size:
314 163 54
StyI C'CwwG_G
Cuts at: 0 74 299 531
Size: 74 225 232
Fragments arranged by size:
232 225 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 531
Size: 372 32 127
Fragments arranged by size:
372 127 32
XmnI GAAnn'nnTTC
Cuts at: 0 169 212 531
Size: 169 43 319
Fragments arranged by size:
319 169 43
Enzymes that do cut:
AccI AcuI ApoI AvaI BbsI BglII BplI BpuEI
BsaI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BssSI BstYI
Bsu36I BtgI BtsI EaeI EciI Eco57MI EcoO109I Hin4I
HincII HindIII MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PstI SfcI SmlI StyI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI