Restriction map for
pFN21AE3033
(Linear) (Six-base) MAPSORT of: KIEE3033.seq Check: 8426 from: 1 to: 453
KIEE3033.orf
With 182 enzymes: SgfI *
March 22, 2012 16:04 ..
AccI GT'mk_AC
Cuts at: 0 116 453
Size: 116 337
Acc65I G'GTAC_C
Cuts at: 0 136 453
Size: 136 317
AfeI AGC'GCT
Cuts at: 0 305 453
Size: 305 148
AflIII A'CryG_T
Cuts at: 0 400 453
Size: 400 53
ApaLI G'TGCA_C
Cuts at: 0 128 453
Size: 128 325
ApoI r'AATT_y
Cuts at: 0 93 157 175 453
Size: 93 64 18 278
Fragments arranged by size:
278 93 64 18
BanI G'GyrC_C
Cuts at: 0 136 453
Size: 136 317
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 453
Size: 296 157
Bme1580I G_kGCm'C
Cuts at: 0 132 453
Size: 132 321
BsaHI Gr'CG_yC
Cuts at: 0 4 453
Size: 4 449
BsaWI w'CCGG_w
Cuts at: 0 375 453
Size: 375 78
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 194 453
Size: 194 259
BseYI C'CCAG_C
Cuts at: 0 300 453
Size: 300 153
BsiHKAI G_wGCw'C
Cuts at: 0 132 453
Size: 132 321
BsmBI CGTCTCn'nnnn_
Cuts at: 0 360 453
Size: 360 93
Bsp1286I G_dGCh'C
Cuts at: 0 132 453
Size: 132 321
BspHI T'CATG_A
Cuts at: 0 438 453
Size: 438 15
BsrDI GCAATG_nn'
Cuts at: 0 264 453
Size: 264 189
Bsu36I CC'TnA_GG
Cuts at: 0 206 453
Size: 206 247
DraI TTT'AAA
Cuts at: 0 174 453
Size: 174 279
EarI CTCTTCn'nnn_
Cuts at: 0 76 453
Size: 76 377
EcoRV GAT'ATC
Cuts at: 0 190 453
Size: 190 263
HaeII r_GCGC'y
Cuts at: 0 307 453
Size: 307 146
KpnI G_GTAC'C
Cuts at: 0 140 453
Size: 140 313
MslI CAynn'nnrTG
Cuts at: 0 245 453
Size: 245 208
MspA1I CmG'CkG
Cuts at: 0 353 453
Size: 353 100
NspI r_CATG'y
Cuts at: 0 345 404 453
Size: 345 59 49
Fragments arranged by size:
345 59 49
PciI A'CATG_T
Cuts at: 0 400 453
Size: 400 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 61 453
Size: 61 392
PfoI T'CCnGG_A
Cuts at: 0 60 453
Size: 60 393
PvuII CAG'CTG
Cuts at: 0 353 453
Size: 353 100
SexAI A'CCwGG_T
Cuts at: 0 132 453
Size: 132 321
SfcI C'TryA_G
Cuts at: 0 22 453
Size: 22 431
SphI G_CATG'C
Cuts at: 0 345 453
Size: 345 108
SspI AAT'ATT
Cuts at: 0 231 453
Size: 231 222
SwaI ATTT'AAAT
Cuts at: 0 174 453
Size: 174 279
XmnI GAAnn'nnTTC
Cuts at: 0 158 230 453
Size: 158 72 223
Fragments arranged by size:
223 158 72
Enzymes that do cut:
AccI Acc65I AfeI AflIII ApaLI ApoI BanI BbsI
Bme1580I BsaHI BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspHI BsrDI Bsu36I DraI EarI EcoRV HaeII KpnI
MslI MspA1I NspI PciI PflMI PfoI PvuII SexAI
SfcI SphI SspI SwaI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BsgI BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI