(Linear) (Six-base) MAPSORT of: KIEE3030.seq  Check: 6504  from: 1  to: 468



KIEE3030.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     412     468 
   Size:        130     282      56

  Fragments arranged by size: 

                 282     130      56
 
AflIII A'CryG_T

Cuts at:      0     320     468 
   Size:        320     148
 
ApaI G_GGCC'C

Cuts at:      0     312     468 
   Size:        312     156
 
ApoI r'AATT_y

Cuts at:      0     382     468 
   Size:        382      86
 
AvaI C'yCGr_G

Cuts at:      0     311     468 
   Size:        311     157
 
BanI G'GyrC_C

Cuts at:      0     247     445     468 
   Size:        247     198      23

  Fragments arranged by size: 

                 247     198      23
 
BanII G_rGCy'C

Cuts at:      0     187     312     468 
   Size:        187     125     156

  Fragments arranged by size: 

                 187     156     125
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     143     468 
   Size:        143     325
 
BglI GCCn_nnn'nGGC

Cuts at:      0     173     468 
   Size:        173     295
 
Bme1580I G_kGCm'C

Cuts at:      0     312     468 
   Size:        312     156
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     201     468 
   Size:        201     267
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     468 
   Size:        130     338
 
BseYI C'CCAG_C

Cuts at:      0     285     468 
   Size:        285     183
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     312     468 
   Size:        187     125     156

  Fragments arranged by size: 

                 187     156     125
 
Bsu36I CC'TnA_GG

Cuts at:      0     235     468 
   Size:        235     233
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     412     468 
   Size:        130     282      56

  Fragments arranged by size: 

                 282     130      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     175     468 
   Size:        175     293
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     396     428     468 
   Size:        396      32      40

  Fragments arranged by size: 

                 396      40      32
 
PspOMI G'GGCC_C

Cuts at:      0     308     468 
   Size:        308     160
 
ScaI AGT'ACT

Cuts at:      0     127     468 
   Size:        127     341
 
SmaI CCC'GGG

Cuts at:      0     313     468 
   Size:        313     155
 
SmlI C'TyrA_G

Cuts at:      0     145     468 
   Size:        145     323
 
StyI C'CwwG_G

Cuts at:      0     200     468 
   Size:        200     268
 
TatI w'GTAC_w

Cuts at:      0     125     468 
   Size:        125     343
 
XmaI C'CCGG_G

Cuts at:      0     311     468 
   Size:        311     157
 

 Enzymes that do cut:

     AcuI   AflIII     ApaI     ApoI     AvaI     BanI    BanII     BbsI
     BglI Bme1580I     BplI    BpuEI    BseYI Bsp1286I   Bsu36I  Eco57MI
    EcoNI    Hin4I   PspOMI     ScaI     SmaI     SmlI     StyI     TatI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI