(Linear) (Six-base) MAPSORT of: KIEE3026.seq  Check: 6043  from: 1  to: 438



KIEE3026.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     438 
   Size:        269     169
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      13      45     438 
   Size:         13      32     393

  Fragments arranged by size: 

                 393      32      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     271     438 
   Size:        271     167
 
ApoI r'AATT_y

Cuts at:      0     141     197     438 
   Size:        141      56     241

  Fragments arranged by size: 

                 241     141      56
 
BanII G_rGCy'C

Cuts at:      0      50     438 
   Size:         50     388
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     234     438 
   Size:        234     204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     371     394     438 
   Size:        336      35      23      44

  Fragments arranged by size: 

                 336      44      35      23
 
BsaWI w'CCGG_w

Cuts at:      0     113     438 
   Size:        113     325
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      37      43      67     170     200     438 
   Size:         13      24       6      24     103      30     238

  Fragments arranged by size: 

                 238     103      30      24      24      13       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     299     419     438 
   Size:        299     120      19

  Fragments arranged by size: 

                 299     120      19
 
BseYI C'CCAG_C

Cuts at:      0     264     330     410     438 
   Size:        264      66      80      28

  Fragments arranged by size: 

                 264      80      66      28
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     438 
   Size:         50     388
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     438 
   Size:         50     388
 
BspEI T'CCGG_A

Cuts at:      0     113     438 
   Size:        113     325
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128     438 
   Size:        128     310
 
BsrBI CCG'CTC

Cuts at:      0     156     438 
   Size:        156     282
 
Bsu36I CC'TnA_GG

Cuts at:      0     311     438 
   Size:        311     127
 
EaeI y'GGCC_r

Cuts at:      0     157     190     438 
   Size:        157      33     248

  Fragments arranged by size: 

                 248     157      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     332     438 
   Size:        332     106
 
EcoICRI GAG'CTC

Cuts at:      0      48     438 
   Size:         48     390
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     336     371     394     438 
   Size:        269      67      35      23      44

  Fragments arranged by size: 

                 269      67      44      35      23
 
EcoRI G'AATT_C

Cuts at:      0     197     438 
   Size:        197     241
 
PasI CC'CwG_GG

Cuts at:      0     104     438 
   Size:        104     334
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45     438 
   Size:         13      32     393

  Fragments arranged by size: 

                 393      32      13
 
SacI G_AGCT'C

Cuts at:      0      50     438 
   Size:         50     388
 
ScaI AGT'ACT

Cuts at:      0     426     438 
   Size:        426      12
 
StyI C'CwwG_G

Cuts at:      0     245     438 
   Size:        245     193
 
TatI w'GTAC_w

Cuts at:      0     424     438 
   Size:        424      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     170     188     202     220     438 
   Size:        170      18      14      18     218

  Fragments arranged by size: 

                 218     170      18      18      14
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI    BanII     BmrI     BpmI    BsaWI
    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI
   Bsu36I     EaeI     EciI  EcoICRI  Eco57MI    EcoRI     PasI     PpiI
     SacI     ScaI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI