(Linear) (Six-base) MAPSORT of: KIEE3025.seq  Check: 3514  from: 1  to: 426



KIEE3025.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     145     426 
   Size:        145     281
 
AlwNI CAG_nnn'CTG

Cuts at:      0     214     380     383     426 
   Size:        214     166       3      43

  Fragments arranged by size: 

                 214     166      43       3
 
ApoI r'AATT_y

Cuts at:      0     112     426 
   Size:        112     314
 
AvaI C'yCGr_G

Cuts at:      0      74     426 
   Size:         74     352
 
BanII G_rGCy'C

Cuts at:      0     148     426 
   Size:        148     278
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     227     426 
   Size:        227     199
 
BbvCI CC'TCA_GC

Cuts at:      0      68     426 
   Size:         68     358
 
BglI GCCn_nnn'nGGC

Cuts at:      0     181     426 
   Size:        181     245
 
Bpu10I CC'TnA_GC

Cuts at:      0      68     426 
   Size:         68     358
 
BseYI C'CCAG_C

Cuts at:      0     313     426 
   Size:        313     113
 
BsiEI CG_ry'CG

Cuts at:      0     108     426 
   Size:        108     318
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     150     426 
   Size:        150     276
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     426 
   Size:        148     278
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     145     426 
   Size:        145     281
 
BssSI C'ACGA_G

Cuts at:      0     178     426 
   Size:        178     248
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     383     426 
   Size:        383      43
 
BtgI C'CryG_G

Cuts at:      0     251     426 
   Size:        251     175
 
EaeI y'GGCC_r

Cuts at:      0       2      58     105     426 
   Size:          2      56      47     321

  Fragments arranged by size: 

                 321      56      47       2
 
EagI C'GGCC_G

Cuts at:      0     105     426 
   Size:        105     321
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     300     426 
   Size:        300     126
 
EcoRI G'AATT_C

Cuts at:      0     112     426 
   Size:        112     314
 
HaeII r_GCGC'y

Cuts at:      0      85     426 
   Size:         85     341
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     101     113     133     145     152     184     426 
   Size:        101      12      20      12       7      32     242

  Fragments arranged by size: 

                 242     101      32      20      12      12       7
 
MscI TGG'CCA

Cuts at:      0       4     426 
   Size:          4     422
 
MspA1I CmG'CkG

Cuts at:      0     317     380     426 
   Size:        317      63      46

  Fragments arranged by size: 

                 317      63      46
 
NcoI C'CATG_G

Cuts at:      0     251     426 
   Size:        251     175
 
NspI r_CATG'y

Cuts at:      0      15     426 
   Size:         15     411
 
PasI CC'CwG_GG

Cuts at:      0     331     426 
   Size:        331      95
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     266     426 
   Size:        266     160
 
PfoI T'CCnGG_A

Cuts at:      0     200     426 
   Size:        200     226
 
PvuII CAG'CTG

Cuts at:      0     317     380     426 
   Size:        317      63      46

  Fragments arranged by size: 

                 317      63      46
 
SmaI CCC'GGG

Cuts at:      0      76     426 
   Size:         76     350
 
SrfI GCCC'GGGC

Cuts at:      0      76     426 
   Size:         76     350
 
StuI AGG'CCT

Cuts at:      0     295     426 
   Size:        295     131
 
StyI C'CwwG_G

Cuts at:      0     251     426 
   Size:        251     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      27     426 
   Size:         27     399
 
Tth111I GACn'n_nGTC

Cuts at:      0     282     426 
   Size:        282     144
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     267     426 
   Size:        267     159
 
XmaI C'CCGG_G

Cuts at:      0      74     426 
   Size:         74     352
 

 Enzymes that do cut:

     AarI    AlwNI     ApoI     AvaI    BanII     BbsI    BbvCI     BglI
   Bpu10I    BseYI    BsiEI    BsmBI Bsp1286I    BspMI    BssSI   BstAPI
     BtgI     EaeI     EagI    EcoNI    EcoRI    HaeII    Hin4I     MscI
   MspA1I     NcoI     NspI     PasI    PflMI     PfoI    PvuII     SmaI
     SrfI     StuI     StyI    TaqII  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     SwaI     TatI
     TstI     XbaI     XhoI     XmnI     ZraI