Restriction map for
pFN21AE3022
(Linear) (Six-base) MAPSORT of: KIEE3022.seq Check: 5221 from: 1 to: 408
KIEE3022.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 408
Size: 369 39
AlwNI CAG_nnn'CTG
Cuts at: 0 198 408
Size: 198 210
ApaI G_GGCC'C
Cuts at: 0 140 408
Size: 140 268
BanI G'GyrC_C
Cuts at: 0 46 408
Size: 46 362
BanII G_rGCy'C
Cuts at: 0 80 140 356 408
Size: 80 60 216 52
Fragments arranged by size:
216 80 60 52
BbeI G_GCGC'C
Cuts at: 0 50 408
Size: 50 358
BbsI GAAGACnn'nnnn_
Cuts at: 0 325 408
Size: 325 83
BbvCI CC'TCA_GC
Cuts at: 0 347 408
Size: 347 61
BglI GCCn_nnn'nGGC
Cuts at: 0 127 408
Size: 127 281
Bme1580I G_kGCm'C
Cuts at: 0 140 408
Size: 140 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 408
Size: 128 280
Bpu10I CC'TnA_GC
Cuts at: 0 347 408
Size: 347 61
BsaHI Gr'CG_yC
Cuts at: 0 47 86 408
Size: 47 39 322
Fragments arranged by size:
322 47 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 408
Size: 364 44
BseYI C'CCAG_C
Cuts at: 0 42 116 408
Size: 42 74 292
Fragments arranged by size:
292 74 42
BsiEI CG_ry'CG
Cuts at: 0 402 408
Size: 402 6
BsiHKAI G_wGCw'C
Cuts at: 0 356 408
Size: 356 52
Bsp1286I G_dGCh'C
Cuts at: 0 80 140 356 408
Size: 80 60 216 52
Fragments arranged by size:
216 80 60 52
BsrFI r'CCGG_y
Cuts at: 0 292 408
Size: 292 116
BtgI C'CryG_G
Cuts at: 0 89 131 408
Size: 89 42 277
Fragments arranged by size:
277 89 42
BtsI GCAGTG_nn'
Cuts at: 0 288 408
Size: 288 120
DraIII CAC_nnn'GTG
Cuts at: 0 153 408
Size: 153 255
EaeI y'GGCC_r
Cuts at: 0 128 408
Size: 128 280
EarI CTCTTCn'nnn_
Cuts at: 0 37 66 408
Size: 37 29 342
Fragments arranged by size:
342 37 29
EcoICRI GAG'CTC
Cuts at: 0 354 408
Size: 354 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 369 408
Size: 128 241 39
Fragments arranged by size:
241 128 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 408
Size: 346 62
EcoO109I rG'GnC_Cy
Cuts at: 0 136 137 195 408
Size: 136 1 58 213
Fragments arranged by size:
213 136 58 1
HaeII r_GCGC'y
Cuts at: 0 50 408
Size: 50 358
KasI G'GCGC_C
Cuts at: 0 46 408
Size: 46 362
MscI TGG'CCA
Cuts at: 0 130 408
Size: 130 278
MspA1I CmG'CkG
Cuts at: 0 42 326 408
Size: 42 284 82
Fragments arranged by size:
284 82 42
NarI GG'CG_CC
Cuts at: 0 47 408
Size: 47 361
NcoI C'CATG_G
Cuts at: 0 89 131 408
Size: 89 42 277
Fragments arranged by size:
277 89 42
NspI r_CATG'y
Cuts at: 0 244 408
Size: 244 164
PspOMI G'GGCC_C
Cuts at: 0 136 408
Size: 136 272
PstI C_TGCA'G
Cuts at: 0 224 253 408
Size: 224 29 155
Fragments arranged by size:
224 155 29
SacI G_AGCT'C
Cuts at: 0 356 408
Size: 356 52
SfcI C'TryA_G
Cuts at: 0 220 249 363 408
Size: 220 29 114 45
Fragments arranged by size:
220 114 45 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 127 408
Size: 127 281
SfoI GGC'GCC
Cuts at: 0 48 408
Size: 48 360
SphI G_CATG'C
Cuts at: 0 244 408
Size: 244 164
StuI AGG'CCT
Cuts at: 0 9 408
Size: 9 399
StyI C'CwwG_G
Cuts at: 0 89 131 408
Size: 89 42 277
Fragments arranged by size:
277 89 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 388 408
Size: 82 306 20
Fragments arranged by size:
306 82 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 87 408
Size: 87 321
Enzymes that do cut:
AcuI AlwNI ApaI BanI BanII BbeI BbsI BbvCI
BglI Bme1580I BpmI Bpu10I BsaHI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BsrFI BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MscI MspA1I
NarI NcoI NspI PspOMI PstI SacI SfcI SfiI
SfoI SphI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI