(Linear) (Six-base) MAPSORT of: KIEE3015.seq  Check: 8598  from: 1  to: 330



KIEE3015.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     330 
   Size:        308      22
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306     330 
   Size:        306      24
 
BbvCI CC'TCA_GC

Cuts at:      0      24     330 
   Size:         24     306
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     211     245     330 
   Size:        211      34      85

  Fragments arranged by size: 

                 211      85      34
 
BclI T'GATC_A

Cuts at:      0     216     330 
   Size:        216     114
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     286     330 
   Size:        146     140      44

  Fragments arranged by size: 

                 146     140      44
 
Bpu10I CC'TnA_GC

Cuts at:      0      24     330 
   Size:         24     306
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12     330 
   Size:         12     318
 
BseYI C'CCAG_C

Cuts at:      0     306     330 
   Size:        306      24
 
BstYI r'GATC_y

Cuts at:      0      58     330 
   Size:         58     272
 
BtgI C'CryG_G

Cuts at:      0      91     330 
   Size:         91     239
 
EarI CTCTTCn'nnn_

Cuts at:      0     254     327     330 
   Size:        254      73       3

  Fragments arranged by size: 

                 254      73       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     286     308     330 
   Size:        146     140      22      22

  Fragments arranged by size: 

                 146     140      22      22
 
MspA1I CmG'CkG

Cuts at:      0      28     303     330 
   Size:         28     275      27

  Fragments arranged by size: 

                 275      28      27
 
PvuII CAG'CTG

Cuts at:      0      28     303     330 
   Size:         28     275      27

  Fragments arranged by size: 

                 275      28      27
 
SapI GCTCTTCn'nnn_

Cuts at:      0     254     330 
   Size:        254      76
 

 Enzymes that do cut:

     AcuI    AlwNI    BbvCI     BcgI     BclI     BpmI   Bpu10I    BseRI
    BseYI    BstYI     BtgI     EarI  Eco57MI   MspA1I    PvuII     SapI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI