(Linear) (Six-base) MAPSORT of: KIEE3014.seq  Check: 1006  from: 1  to: 312



KIEE3014.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     128     256     312 
   Size:        122       6     128      56

  Fragments arranged by size: 

                 128     122      56       6
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      37      69     312 
   Size:         37      32     243

  Fragments arranged by size: 

                 243      37      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     312 
   Size:        132     180
 
ApoI r'AATT_y

Cuts at:      0     251     312 
   Size:        251      61
 
BanII G_rGCy'C

Cuts at:      0       7     312 
   Size:          7     305
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     148     312 
   Size:        148     164
 
BclI T'GATC_A

Cuts at:      0     188     312 
   Size:        188     124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     312 
   Size:        202     110
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      37      67     312 
   Size:         37      30     245

  Fragments arranged by size: 

                 245      37      30
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     312 
   Size:          7     305
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     312 
   Size:          7     305
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     131     312 
   Size:        131     181
 
BsrGI T'GTAC_A

Cuts at:      0     278     312 
   Size:        278      34
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     273     312 
   Size:        273      39
 
BstYI r'GATC_y

Cuts at:      0     261     312 
   Size:        261      51
 
BtsI GCAGTG_nn'

Cuts at:      0     133     204     212     312 
   Size:        133      71       8     100

  Fragments arranged by size: 

                 133     100      71       8
 
EcoICRI GAG'CTC

Cuts at:      0       5     312 
   Size:          5     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     128     202     256     312 
   Size:        122       6      74      54      56

  Fragments arranged by size: 

                 122      74      56      54       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0      17     120     312 
   Size:         17     103     192

  Fragments arranged by size: 

                 192     103      17
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      37      69     312 
   Size:         37      32     243

  Fragments arranged by size: 

                 243      37      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      17     120     312 
   Size:         17     103     192

  Fragments arranged by size: 

                 192     103      17
 
PstI C_TGCA'G

Cuts at:      0     128     312 
   Size:        128     184
 
SacI G_AGCT'C

Cuts at:      0       7     312 
   Size:          7     305
 
SfcI C'TryA_G

Cuts at:      0     124     312 
   Size:        124     188
 
TatI w'GTAC_w

Cuts at:      0     278     312 
   Size:        278      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     112     144     312 
   Size:        112      32     168

  Fragments arranged by size: 

                 168     112      32
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI    BanII     BbsI     BclI     BpmI
    BsaXI  BsiHKAI Bsp1286I    BspMI    BsrGI   BstAPI    BstYI     BtsI
  EcoICRI  Eco57MI EcoO109I     PpiI    PpuMI     PstI     SacI     SfcI
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI