(Linear) (Six-base) MAPSORT of: KIEE3013.seq  Check: 775  from: 1  to: 309



KIEE3013.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      88     309 
   Size:         88     221
 
AleI CACnn'nnGTG

Cuts at:      0     160     309 
   Size:        160     149
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27     227     309 
   Size:         27     200      82

  Fragments arranged by size: 

                 200      82      27
 
BanII G_rGCy'C

Cuts at:      0      92     199     309 
   Size:         92     107     110

  Fragments arranged by size: 

                 110     107      92
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     275     309 
   Size:        275      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     204     309 
   Size:         63     141     105

  Fragments arranged by size: 

                 141     105      63
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     309 
   Size:         52     257
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     281     309 
   Size:        281      28
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     163     193     309 
   Size:        163      30     116

  Fragments arranged by size: 

                 163     116      30
 
BseYI C'CCAG_C

Cuts at:      0      11     309 
   Size:         11     298
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     309 
   Size:         40     269
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     199     309 
   Size:         92     107     110

  Fragments arranged by size: 

                 110     107      92
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      88     309 
   Size:         88     221
 
BsrBI CCG'CTC

Cuts at:      0     212     309 
   Size:        212      97
 
BssSI C'ACGA_G

Cuts at:      0      31     309 
   Size:         31     278
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      27     309 
   Size:         27     282
 
BstEII G'GTnAC_C

Cuts at:      0     204     309 
   Size:        204     105
 
BtgI C'CryG_G

Cuts at:      0     200     309 
   Size:        200     109
 
EaeI y'GGCC_r

Cuts at:      0     233     309 
   Size:        233      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     204     309 
   Size:         63     141     105

  Fragments arranged by size: 

                 141     105      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     309 
   Size:         63     246
 
MscI TGG'CCA

Cuts at:      0     235     309 
   Size:        235      74
 
MslI CAynn'nnrTG

Cuts at:      0     160     309 
   Size:        160     149
 
MspA1I CmG'CkG

Cuts at:      0      24     125     309 
   Size:         24     101     184

  Fragments arranged by size: 

                 184     101      24
 
PstI C_TGCA'G

Cuts at:      0     112     309 
   Size:        112     197
 
PvuII CAG'CTG

Cuts at:      0      24     309 
   Size:         24     285
 
SbfI CC_TGCA'GG

Cuts at:      0     112     309 
   Size:        112     197
 
SfcI C'TryA_G

Cuts at:      0     108     309 
   Size:        108     201
 
SmlI C'TyrA_G

Cuts at:      0      67     309 
   Size:         67     242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     228     309 
   Size:        228      81
 

 Enzymes that do cut:

     AarI     AleI    AlwNI    BanII     BcgI     BpmI    BpuEI     BsaI
    BsaXI    BseYI     BsgI Bsp1286I    BspMI    BsrBI    BssSI   BstAPI
   BstEII     BtgI     EaeI  Eco57MI EcoO109I     MscI     MslI   MspA1I
     PstI    PvuII     SbfI     SfcI     SmlI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI