(Linear) (Six-base) MAPSORT of: KIEE3011.seq  Check: 5880  from: 1  to: 297



KIEE3011.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AclI AA'CG_TT

Cuts at:      0      77     297 
   Size:         77     220
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     297 
   Size:         18     279
 
ApoI r'AATT_y

Cuts at:      0     244     297 
   Size:        244      53
 
AseI AT'TA_AT

Cuts at:      0     107     297 
   Size:        107     190
 
BglII A'GATC_T

Cuts at:      0     138     297 
   Size:        138     159
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     127     297 
   Size:         40      87     170

  Fragments arranged by size: 

                 170      87      40
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      70     297 
   Size:         70     227
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      24     297 
   Size:         24     273
 
BssSI C'ACGA_G

Cuts at:      0     158     297 
   Size:        158     139
 
BstYI r'GATC_y

Cuts at:      0     138     297 
   Size:        138     159
 
DraI TTT'AAA

Cuts at:      0     266     297 
   Size:        266      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     127     297 
   Size:         40      87     170

  Fragments arranged by size: 

                 170      87      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     297 
   Size:        130      32     135

  Fragments arranged by size: 

                 135     130      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     194     226     297 
   Size:        194      32      71

  Fragments arranged by size: 

                 194      71      32
 

 Enzymes that do cut:

     AclI    AlwNI     ApoI     AseI    BglII     BpmI     BsaI    BspMI
    BssSI    BstYI     DraI  Eco57MI    Hin4I     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI