(Linear) (Six-base) MAPSORT of: KIEE3004.seq  Check: 6299  from: 1  to: 228



KIEE3004.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AhdI GACnn_n'nnGTC

Cuts at:      0      53     228 
   Size:         53     175
 
AlwNI CAG_nnn'CTG

Cuts at:      0      94     228 
   Size:         94     134
 
AvaI C'yCGr_G

Cuts at:      0     139     228 
   Size:        139      89
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     228 
   Size:        137      91
 
BsaWI w'CCGG_w

Cuts at:      0      44     228 
   Size:         44     184
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      19      28      49     228 
   Size:         19       9      21     179

  Fragments arranged by size: 

                 179      21      19       9
 
BsmI GAATG_Cn'

Cuts at:      0      66     228 
   Size:         66     162
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     224     228 
   Size:        224       4
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     222     228 
   Size:        222       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     228 
   Size:        137      91
 
HaeII r_GCGC'y

Cuts at:      0     173     228 
   Size:        173      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      30      39      51      71     228 
   Size:         19      11       9      12      20     157

  Fragments arranged by size: 

                 157      20      19      12      11       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     228 
   Size:         37     191
 
PspXI vC'TCGA_Gb

Cuts at:      0     139     228 
   Size:        139      89
 
SmlI C'TyrA_G

Cuts at:      0     139     228 
   Size:        139      89
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     208     228 
   Size:        208      20
 
XhoI C'TCGA_G

Cuts at:      0     139     228 
   Size:        139      89
 

 Enzymes that do cut:

     AhdI    AlwNI     AvaI     BpmI    BsaWI    BsaXI     BsmI    BtgZI
     DrdI  Eco57MI    HaeII    Hin4I     MmeI    PspXI     SmlI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XmaI     XmnI     ZraI