(Linear) (Six-base) MAPSORT of: KIEE3001.seq  Check: 777  from: 1  to: 204



KIEE3001.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:01  ..

ApoI r'AATT_y

Cuts at:      0     161     204 
   Size:        161      43
 
BanI G'GyrC_C

Cuts at:      0     169     204 
   Size:        169      35
 
BclI T'GATC_A

Cuts at:      0      96     204 
   Size:         96     108
 
BglII A'GATC_T

Cuts at:      0      91     204 
   Size:         91     113
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      72     204 
   Size:         72     132
 
BsrDI GCAATG_nn'

Cuts at:      0     133     204 
   Size:        133      71
 
BstYI r'GATC_y

Cuts at:      0      91     204 
   Size:         91     113
 
Bsu36I CC'TnA_GG

Cuts at:      0      57     204 
   Size:         57     147
 
BtsI GCAGTG_nn'

Cuts at:      0     123     204 
   Size:        123      81
 
EaeI y'GGCC_r

Cuts at:      0      84     204 
   Size:         84     120
 
MfeI C'AATT_G

Cuts at:      0     105     204 
   Size:        105      99
 
MscI TGG'CCA

Cuts at:      0      86     204 
   Size:         86     118
 
StuI AGG'CCT

Cuts at:      0      80     204 
   Size:         80     124
 
StyI C'CwwG_G

Cuts at:      0      81     204 
   Size:         81     123
 

 Enzymes that do cut:

     ApoI     BanI     BclI    BglII     BplI    BsrDI    BstYI   Bsu36I
     BtsI     EaeI     MfeI     MscI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI