Restriction map for
pFN21AE2800
(Linear) (Six-base) MAPSORT of: KIEE2800.seq Check: 9108 from: 1 to: 1491
KIEE2800.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1170 1491
Size: 1170 321
AflIII A'CryG_T
Cuts at: 0 1199 1491
Size: 1199 292
AhdI GACnn_n'nnGTC
Cuts at: 0 759 1491
Size: 759 732
AleI CACnn'nnGTG
Cuts at: 0 496 1491
Size: 496 995
AlwNI CAG_nnn'CTG
Cuts at: 0 360 409 954 1308 1491
Size: 360 49 545 354 183
Fragments arranged by size:
545 360 354 183 49
ApaI G_GGCC'C
Cuts at: 0 909 1491
Size: 909 582
ApoI r'AATT_y
Cuts at: 0 215 382 1491
Size: 215 167 1109
Fragments arranged by size:
1109 215 167
AvaI C'yCGr_G
Cuts at: 0 68 1491
Size: 68 1423
AvrII C'CTAG_G
Cuts at: 0 522 1491
Size: 522 969
BanII G_rGCy'C
Cuts at: 0 152 317 517 909 1491
Size: 152 165 200 392 582
Fragments arranged by size:
582 392 200 165 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 198 1176 1491
Size: 198 978 315
Fragments arranged by size:
978 315 198
BbvCI CC'TCA_GC
Cuts at: 0 1243 1491
Size: 1243 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 87 121 536 570 1491
Size: 87 34 415 34 921
Fragments arranged by size:
921 415 87 34 34
BfrBI ATG'CAT
Cuts at: 0 1171 1491
Size: 1171 320
BlpI GC'TnA_GC
Cuts at: 0 316 1491
Size: 316 1175
Bme1580I G_kGCm'C
Cuts at: 0 11 909 1491
Size: 11 898 582
Fragments arranged by size:
898 582 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 1036 1491
Size: 1036 455
Bpu10I CC'TnA_GC
Cuts at: 0 1243 1491
Size: 1243 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1460 1491
Size: 1460 31
BsaWI w'CCGG_w
Cuts at: 0 804 1491
Size: 804 687
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 861 1491
Size: 799 62 630
Fragments arranged by size:
799 630 62
BseYI C'CCAG_C
Cuts at: 0 171 723 746 1491
Size: 171 552 23 745
Fragments arranged by size:
745 552 171 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1442 1491
Size: 1442 49
BsiEI CG_ry'CG
Cuts at: 0 101 1491
Size: 101 1390
BsiHKAI G_wGCw'C
Cuts at: 0 56 1491
Size: 56 1435
BsmI GAATG_Cn'
Cuts at: 0 88 1491
Size: 88 1403
BsmBI CGTCTCn'nnnn_
Cuts at: 0 829 1491
Size: 829 662
Bsp1286I G_dGCh'C
Cuts at: 0 11 56 152 317 517 909 1491
Size: 11 45 96 165 200 392 582
Fragments arranged by size:
582 392 200 165 96 45 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 676 1491
Size: 176 500 815
Fragments arranged by size:
815 500 176
BsrBI CCG'CTC
Cuts at: 0 67 1491
Size: 67 1424
BsrDI GCAATG_nn'
Cuts at: 0 1286 1491
Size: 1286 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1308 1491
Size: 1308 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 498 742 1491
Size: 498 244 749
Fragments arranged by size:
749 498 244
BstYI r'GATC_y
Cuts at: 0 1458 1491
Size: 1458 33
Bsu36I CC'TnA_GG
Cuts at: 0 136 1491
Size: 136 1355
BtgI C'CryG_G
Cuts at: 0 794 1491
Size: 794 697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 381 1380 1491
Size: 381 999 111
Fragments arranged by size:
999 381 111
BtsI GCAGTG_nn'
Cuts at: 0 1133 1297 1491
Size: 1133 164 194
Fragments arranged by size:
1133 194 164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 396 431 1491
Size: 396 35 1060
Fragments arranged by size:
1060 396 35
EaeI y'GGCC_r
Cuts at: 0 44 365 1412 1491
Size: 44 321 1047 79
Fragments arranged by size:
1047 321 79 44
EarI CTCTTCn'nnn_
Cuts at: 0 631 649 887 1491
Size: 631 18 238 604
Fragments arranged by size:
631 604 238 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1170 1491
Size: 1170 321
EcoO109I rG'GnC_Cy
Cuts at: 0 906 951 1491
Size: 906 45 540
Fragments arranged by size:
906 540 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 509 660 1491
Size: 509 151 831
Fragments arranged by size:
831 509 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 777 960 992 1083 1115 1450 1482
Size: 745 32 183 32 91 32 335 32
Cuts at: 1482 1491
Size: 9
Fragments arranged by size:
745 335 183 91 32 32 32 32
9
HincII GTy'rAC
Cuts at: 0 1076 1491
Size: 1076 415
MscI TGG'CCA
Cuts at: 0 367 1414 1491
Size: 367 1047 77
Fragments arranged by size:
1047 367 77
MslI CAynn'nnrTG
Cuts at: 0 496 1159 1198 1399 1491
Size: 496 663 39 201 92
Fragments arranged by size:
663 496 201 92 39
NruI TCG'CGA
Cuts at: 0 80 1491
Size: 80 1411
NsiI A_TGCA'T
Cuts at: 0 1173 1491
Size: 1173 318
NspI r_CATG'y
Cuts at: 0 459 1203 1491
Size: 459 744 288
Fragments arranged by size:
744 459 288
PasI CC'CwG_GG
Cuts at: 0 354 1491
Size: 354 1137
PciI A'CATG_T
Cuts at: 0 1199 1491
Size: 1199 292
PsiI TTA'TAA
Cuts at: 0 290 1491
Size: 290 1201
PspOMI G'GGCC_C
Cuts at: 0 905 1491
Size: 905 586
PstI C_TGCA'G
Cuts at: 0 311 1128 1304 1491
Size: 311 817 176 187
Fragments arranged by size:
817 311 187 176
RsrII CG'GwC_CG
Cuts at: 0 62 1491
Size: 62 1429
SapI GCTCTTCn'nnn_
Cuts at: 0 631 1491
Size: 631 860
ScaI AGT'ACT
Cuts at: 0 842 1491
Size: 842 649
SexAI A'CCwGG_T
Cuts at: 0 242 1491
Size: 242 1249
SfcI C'TryA_G
Cuts at: 0 307 435 1124 1300 1491
Size: 307 128 689 176 191
Fragments arranged by size:
689 307 191 176 128
SmlI C'TyrA_G
Cuts at: 0 1475 1491
Size: 1475 16
StyI C'CwwG_G
Cuts at: 0 522 1341 1491
Size: 522 819 150
Fragments arranged by size:
819 522 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 1018 1149 1491
Size: 208 810 131 342
Fragments arranged by size:
810 342 208 131
TatI w'GTAC_w
Cuts at: 0 840 1491
Size: 840 651
XbaI T'CTAG_A
Cuts at: 0 378 1491
Size: 378 1113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 679 1491
Size: 679 812
XmnI GAAnn'nnTTC
Cuts at: 0 853 1491
Size: 853 638
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaI ApoI AvaI
AvrII BanII BbsI BbvCI BcgI BfrBI BlpI Bme1580I
BmrI Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HincII MscI MslI
NruI NsiI NspI PasI PciI PsiI PspOMI PstI
RsrII SapI ScaI SexAI SfcI SmlI StyI TaqII
TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BanI BbeI BciVI BclI BglI BglII BmgBI BmtI
BplI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BsiWI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI PacI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI
PvuI PvuII SacI SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XhoI XmaI ZraI