Restriction map for
pFN21AE2797
(Linear) (Six-base) MAPSORT of: KIEE2797.seq Check: 3481 from: 1 to: 2373
KIEE2797.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AatII G_ACGT'C
Cuts at: 0 722 2373
Size: 722 1651
AccI GT'mk_AC
Cuts at: 0 1223 2373
Size: 1223 1150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 602 1090 1783 1857 2373
Size: 376 226 488 693 74 516
Fragments arranged by size:
693 516 488 376 226 74
AflIII A'CryG_T
Cuts at: 0 1316 2373
Size: 1316 1057
AleI CACnn'nnGTG
Cuts at: 0 1180 2373
Size: 1180 1193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1546 1578 2373
Size: 1546 32 795
Fragments arranged by size:
1546 795 32
AlwNI CAG_nnn'CTG
Cuts at: 0 840 2373
Size: 840 1533
ApaLI G'TGCA_C
Cuts at: 0 119 311 2373
Size: 119 192 2062
Fragments arranged by size:
2062 192 119
ApoI r'AATT_y
Cuts at: 0 2353 2373
Size: 2353 20
AvaI C'yCGr_G
Cuts at: 0 823 1388 2256 2373
Size: 823 565 868 117
Fragments arranged by size:
868 823 565 117
AvrII C'CTAG_G
Cuts at: 0 1452 2373
Size: 1452 921
BamHI G'GATC_C
Cuts at: 0 563 2373
Size: 563 1810
BanI G'GyrC_C
Cuts at: 0 24 443 712 2075 2373
Size: 24 419 269 1363 298
Fragments arranged by size:
1363 419 298 269 24
BbeI G_GCGC'C
Cuts at: 0 447 716 2079 2373
Size: 447 269 1363 294
Fragments arranged by size:
1363 447 294 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 511 1602 2233 2258 2373
Size: 180 331 1091 631 25 115
Fragments arranged by size:
1091 631 331 180 115 25
BbvCI CC'TCA_GC
Cuts at: 0 2131 2373
Size: 2131 242
BciVI GTATCCnnnnn_n'
Cuts at: 0 1958 2373
Size: 1958 415
BfrBI ATG'CAT
Cuts at: 0 2335 2373
Size: 2335 38
BglI GCCn_nnn'nGGC
Cuts at: 0 739 2373
Size: 739 1634
Bme1580I G_kGCm'C
Cuts at: 0 123 315 2373
Size: 123 192 2058
Fragments arranged by size:
2058 192 123
BmgBI CAC'GTC
Cuts at: 0 2235 2373
Size: 2235 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 50 252 302 999 1146 2373
Size: 50 202 50 697 147 1227
Fragments arranged by size:
1227 697 202 147 50 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2067 2373
Size: 2067 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1018 1285 2373
Size: 154 864 267 1088
Fragments arranged by size:
1088 864 267 154
Bpu10I CC'TnA_GC
Cuts at: 0 2131 2373
Size: 2131 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 2373
Size: 251 2122
BsaI GGTCTCn'nnnn_
Cuts at: 0 277 994 1518 2373
Size: 277 717 524 855
Fragments arranged by size:
855 717 524 277
BsaHI Gr'CG_yC
Cuts at: 0 444 713 719 790 2076 2373
Size: 444 269 6 71 1286 297
Fragments arranged by size:
1286 444 297 269 71 6
BsaWI w'CCGG_w
Cuts at: 0 1109 2144 2373
Size: 1109 1035 229
Fragments arranged by size:
1109 1035 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 165 195 235 265 1843 1873 2373
Size: 165 30 40 30 1578 30 500
Fragments arranged by size:
1578 500 165 40 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 254 351 412 643 1408 1411 1993
Size: 170 84 97 61 231 765 3 582
Cuts at: 1993 1996 2148 2373
Size: 3 152 225
Fragments arranged by size:
765 582 231 225 170 152 97 84
61 3 3
BseYI C'CCAG_C
Cuts at: 0 1673 2373
Size: 1673 700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1258 2373
Size: 853 405 1115
Fragments arranged by size:
1115 853 405
BsiHKAI G_wGCw'C
Cuts at: 0 123 315 851 1472 2373
Size: 123 192 536 621 901
Fragments arranged by size:
901 621 536 192 123
BsiWI C'GTAC_G
Cuts at: 0 1142 2373
Size: 1142 1231
BsmBI CGTCTCn'nnnn_
Cuts at: 0 781 1377 2055 2241 2373
Size: 781 596 678 186 132
Fragments arranged by size:
781 678 596 186 132
Bsp1286I G_dGCh'C
Cuts at: 0 123 315 851 1472 2373
Size: 123 192 536 621 901
Fragments arranged by size:
901 621 536 192 123
BspEI T'CCGG_A
Cuts at: 0 1109 2144 2373
Size: 1109 1035 229
Fragments arranged by size:
1109 1035 229
BspHI T'CATG_A
Cuts at: 0 1730 2373
Size: 1730 643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 517 593 2373
Size: 517 76 1780
Fragments arranged by size:
1780 517 76
BsrBI CCG'CTC
Cuts at: 0 672 2373
Size: 672 1701
BsrDI GCAATG_nn'
Cuts at: 0 1505 2018 2373
Size: 1505 513 355
Fragments arranged by size:
1505 513 355
BsrFI r'CCGG_y
Cuts at: 0 90 709 1244 2373
Size: 90 619 535 1129
Fragments arranged by size:
1129 619 535 90
BsrGI T'GTAC_A
Cuts at: 0 1721 2373
Size: 1721 652
BssSI C'ACGA_G
Cuts at: 0 850 881 1651 2373
Size: 850 31 770 722
Fragments arranged by size:
850 770 722 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1254 1281 2373
Size: 1254 27 1092
Fragments arranged by size:
1254 1092 27
BstEII G'GTnAC_C
Cuts at: 0 909 2373
Size: 909 1464
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 201 1668 1914 2373
Size: 21 180 1467 246 459
Fragments arranged by size:
1467 459 246 180 21
BstYI r'GATC_y
Cuts at: 0 563 1064 1533 2373
Size: 563 501 469 840
Fragments arranged by size:
840 563 501 469
BtgI C'CryG_G
Cuts at: 0 200 914 1193 1661 1712 2373
Size: 200 714 279 468 51 661
Fragments arranged by size:
714 661 468 279 200 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 645 672 687 2373
Size: 645 27 15 1686
Fragments arranged by size:
1686 645 27 15
BtsI GCAGTG_nn'
Cuts at: 0 226 1889 2373
Size: 226 1663 484
Fragments arranged by size:
1663 484 226
EaeI y'GGCC_r
Cuts at: 0 320 917 1040 1658 2168 2373
Size: 320 597 123 618 510 205
Fragments arranged by size:
618 597 510 320 205 123
EarI CTCTTCn'nnn_
Cuts at: 0 79 109 323 578 1900 2373
Size: 79 30 214 255 1322 473
Fragments arranged by size:
1322 473 255 214 79 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 1075 2373
Size: 109 966 1298
Fragments arranged by size:
1298 966 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 376 602 1018 1090 1285 1783 1857
Size: 154 222 226 416 72 195 498 74
Cuts at: 1857 2373
Size: 516
Fragments arranged by size:
516 498 416 226 222 195 154 74
72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 517 2373
Size: 517 1856
EcoO109I rG'GnC_Cy
Cuts at: 0 740 2260 2373
Size: 740 1520 113
Fragments arranged by size:
1520 740 113
EcoRI G'AATT_C
Cuts at: 0 2353 2373
Size: 2353 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 384 759 1609 2373
Size: 384 375 850 764
Fragments arranged by size:
850 764 384 375
HaeII r_GCGC'y
Cuts at: 0 447 655 716 2079 2373
Size: 447 208 61 1363 294
Fragments arranged by size:
1363 447 294 208 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 165 197 235 267 274 306
Size: 12 32 121 32 38 32 7 32
Cuts at: 306 853 885 1525 1557 1843 1875 2373
Size: 547 32 640 32 286 32 498
Fragments arranged by size:
640 547 498 286 121 38 32 32
32 32 32 32 32 12 7
HincII GTy'rAC
Cuts at: 0 723 2373
Size: 723 1650
KasI G'GCGC_C
Cuts at: 0 443 712 2075 2373
Size: 443 269 1363 298
Fragments arranged by size:
1363 443 298 269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 518 2208 2373
Size: 233 285 1690 165
Fragments arranged by size:
1690 285 233 165
MscI TGG'CCA
Cuts at: 0 322 1660 2373
Size: 322 1338 713
Fragments arranged by size:
1338 713 322
MslI CAynn'nnrTG
Cuts at: 0 1180 1492 1717 2373
Size: 1180 312 225 656
Fragments arranged by size:
1180 656 312 225
MspA1I CmG'CkG
Cuts at: 0 318 837 1032 1038 1272 2373
Size: 318 519 195 6 234 1101
Fragments arranged by size:
1101 519 318 234 195 6
NaeI GCC'GGC
Cuts at: 0 92 711 2373
Size: 92 619 1662
Fragments arranged by size:
1662 619 92
NarI GG'CG_CC
Cuts at: 0 444 713 2076 2373
Size: 444 269 1363 297
Fragments arranged by size:
1363 444 297 269
NcoI C'CATG_G
Cuts at: 0 200 1661 1712 2373
Size: 200 1461 51 661
Fragments arranged by size:
1461 661 200 51
NdeI CA'TA_TG
Cuts at: 0 1289 2373
Size: 1289 1084
NgoMIV G'CCGG_C
Cuts at: 0 90 709 2373
Size: 90 619 1664
Fragments arranged by size:
1664 619 90
NsiI A_TGCA'T
Cuts at: 0 2337 2373
Size: 2337 36
NspI r_CATG'y
Cuts at: 0 812 960 1320 1686 1722 2016 2373
Size: 812 148 360 366 36 294 357
Fragments arranged by size:
812 366 360 357 294 148 36
PasI CC'CwG_GG
Cuts at: 0 1668 2373
Size: 1668 705
PciI A'CATG_T
Cuts at: 0 1316 2373
Size: 1316 1057
PflMI CCAn_nnn'nTGG
Cuts at: 0 1713 2373
Size: 1713 660
PfoI T'CCnGG_A
Cuts at: 0 131 1120 2305 2357 2373
Size: 131 989 1185 52 16
Fragments arranged by size:
1185 989 131 52 16
PshAI GACnn'nnGTC
Cuts at: 0 1152 1369 2233 2373
Size: 1152 217 864 140
Fragments arranged by size:
1152 864 217 140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1672 1704 2373
Size: 1672 32 669
Fragments arranged by size:
1672 669 32
PstI C_TGCA'G
Cuts at: 0 428 1037 1448 1541 1858 2084 2373
Size: 428 609 411 93 317 226 289
Fragments arranged by size:
609 428 411 317 289 226 93
PvuII CAG'CTG
Cuts at: 0 318 837 1038 2373
Size: 318 519 201 1335
Fragments arranged by size:
1335 519 318 201
SapI GCTCTTCn'nnn_
Cuts at: 0 323 2373
Size: 323 2050
SfcI C'TryA_G
Cuts at: 0 424 1033 1444 1537 1854 2080 2373
Size: 424 609 411 93 317 226 293
Fragments arranged by size:
609 424 411 317 293 226 93
SfoI GGC'GCC
Cuts at: 0 445 714 2077 2373
Size: 445 269 1363 296
Fragments arranged by size:
1363 445 296 269
SgrAI Cr'CCGG_yG
Cuts at: 0 709 2373
Size: 709 1664
SmaI CCC'GGG
Cuts at: 0 1390 2258 2373
Size: 1390 868 115
Fragments arranged by size:
1390 868 115
SmlI C'TyrA_G
Cuts at: 0 266 2373
Size: 266 2107
SphI G_CATG'C
Cuts at: 0 812 2373
Size: 812 1561
StuI AGG'CCT
Cuts at: 0 523 634 1913 2373
Size: 523 111 1279 460
Fragments arranged by size:
1279 523 460 111
StyI C'CwwG_G
Cuts at: 0 14 200 323 548 734 1415 1452 1661
Size: 14 186 123 225 186 681 37 209
Cuts at: 1661 1712 1811 2054 2373
Size: 51 99 243 319
Fragments arranged by size:
681 319 243 225 209 186 186 123
99 51 37 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1906 2373
Size: 1906 467
TatI w'GTAC_w
Cuts at: 0 620 1073 1616 1721 2373
Size: 620 453 543 105 652
Fragments arranged by size:
652 620 543 453 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 113 145 2373
Size: 113 32 2228
Fragments arranged by size:
2228 113 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 2097 2373
Size: 315 1782 276
Fragments arranged by size:
1782 315 276
XmaI C'CCGG_G
Cuts at: 0 1388 2256 2373
Size: 1388 868 117
Fragments arranged by size:
1388 868 117
ZraI GAC'GTC
Cuts at: 0 720 2373
Size: 720 1653
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AloI AlwNI ApaLI
ApoI AvaI AvrII BamHI BanI BbeI BbsI BbvCI
BciVI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PshAI
PsrI PstI PvuII SapI SfcI SfoI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaI AscI AseI AsiSI BaeI BanII BcgI BclI
BglII BlpI BmtI BsaAI BsaBI BsiEI BsmI BssHII
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NheI NotI NruI PacI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI