Restriction map for
pFN21AE2793
(Linear) (Six-base) MAPSORT of: KIEE2793.seq Check: 6883 from: 1 to: 1518
KIEE2793.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 243 1006 1518
Size: 243 763 512
Fragments arranged by size:
763 512 243
AatII G_ACGT'C
Cuts at: 0 215 1518
Size: 215 1303
AccI GT'mk_AC
Cuts at: 0 200 1518
Size: 200 1318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 484 1518
Size: 256 228 1034
Fragments arranged by size:
1034 256 228
AflII C'TTAA_G
Cuts at: 0 121 1518
Size: 121 1397
AflIII A'CryG_T
Cuts at: 0 161 275 1518
Size: 161 114 1243
Fragments arranged by size:
1243 161 114
AhdI GACnn_n'nnGTC
Cuts at: 0 330 1518
Size: 330 1188
AlwNI CAG_nnn'CTG
Cuts at: 0 998 1518
Size: 998 520
ApaI G_GGCC'C
Cuts at: 0 272 1518
Size: 272 1246
ApoI r'AATT_y
Cuts at: 0 980 1518
Size: 980 538
AvaI C'yCGr_G
Cuts at: 0 340 1518
Size: 340 1178
BamHI G'GATC_C
Cuts at: 0 689 1518
Size: 689 829
BanI G'GyrC_C
Cuts at: 0 93 1518
Size: 93 1425
BanII G_rGCy'C
Cuts at: 0 272 632 653 1518
Size: 272 360 21 865
Fragments arranged by size:
865 360 272 21
BclI T'GATC_A
Cuts at: 0 758 1518
Size: 758 760
BglI GCCn_nnn'nGGC
Cuts at: 0 1427 1449 1518
Size: 1427 22 69
Fragments arranged by size:
1427 69 22
BlpI GC'TnA_GC
Cuts at: 0 626 718 1168 1518
Size: 626 92 450 350
Fragments arranged by size:
626 450 350 92
Bme1580I G_kGCm'C
Cuts at: 0 272 1014 1518
Size: 272 742 504
Fragments arranged by size:
742 504 272
BmrI ACTGGGnnnn_n'
Cuts at: 0 137 1217 1326 1518
Size: 137 1080 109 192
Fragments arranged by size:
1080 192 137 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1119 1518
Size: 1119 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 991 1518
Size: 835 156 527
Fragments arranged by size:
835 527 156
BsaAI yAC'GTr
Cuts at: 0 276 1518
Size: 276 1242
BsaBI GATnn'nnATC
Cuts at: 0 178 1518
Size: 178 1340
BsaHI Gr'CG_yC
Cuts at: 0 44 212 1518
Size: 44 168 1306
Fragments arranged by size:
1306 168 44
BsaWI w'CCGG_w
Cuts at: 0 1391 1518
Size: 1391 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1386 1416 1518
Size: 1386 30 102
Fragments arranged by size:
1386 102 30
BseYI C'CCAG_C
Cuts at: 0 23 39 69 223 1350 1438 1456 1489
Size: 23 16 30 154 1127 88 18 33
Cuts at: 1489 1504 1518
Size: 15 14
Fragments arranged by size:
1127 154 88 33 30 23 18 16
15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1518
Size: 1066 452
BsiHKAI G_wGCw'C
Cuts at: 0 632 1518
Size: 632 886
BsmI GAATG_Cn'
Cuts at: 0 484 738 1518
Size: 484 254 780
Fragments arranged by size:
780 484 254
Bsp1286I G_dGCh'C
Cuts at: 0 272 632 653 1014 1518
Size: 272 360 21 361 504
Fragments arranged by size:
504 361 360 272 21
BspEI T'CCGG_A
Cuts at: 0 1391 1518
Size: 1391 127
BspHI T'CATG_A
Cuts at: 0 80 215 473 512 794 1518
Size: 80 135 258 39 282 724
Fragments arranged by size:
724 282 258 135 80 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 243 992 1006 1518
Size: 105 138 749 14 512
Fragments arranged by size:
749 512 138 105 14
BsrBI CCG'CTC
Cuts at: 0 1256 1518
Size: 1256 262
BsrFI r'CCGG_y
Cuts at: 0 1448 1518
Size: 1448 70
BsrGI T'GTAC_A
Cuts at: 0 278 857 1518
Size: 278 579 661
Fragments arranged by size:
661 579 278
BssHII G'CGCG_C
Cuts at: 0 19 1518
Size: 19 1499
BssSI C'ACGA_G
Cuts at: 0 697 1518
Size: 697 821
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 1518
Size: 417 1101
BstYI r'GATC_y
Cuts at: 0 173 230 689 1518
Size: 173 57 459 829
Fragments arranged by size:
829 459 173 57
Bsu36I CC'TnA_GG
Cuts at: 0 1406 1518
Size: 1406 112
BtgI C'CryG_G
Cuts at: 0 378 1262 1518
Size: 378 884 256
Fragments arranged by size:
884 378 256
DraIII CAC_nnn'GTG
Cuts at: 0 699 1518
Size: 699 819
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 804 1518
Size: 400 404 714
Fragments arranged by size:
714 404 400
EaeI y'GGCC_r
Cuts at: 0 784 1433 1518
Size: 784 649 85
Fragments arranged by size:
784 649 85
EarI CTCTTCn'nnn_
Cuts at: 0 658 1283 1518
Size: 658 625 235
Fragments arranged by size:
658 625 235
EcoICRI GAG'CTC
Cuts at: 0 630 1518
Size: 630 888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 484 835 991 1518
Size: 256 228 351 156 527
Fragments arranged by size:
527 351 256 228 156
EcoO109I rG'GnC_Cy
Cuts at: 0 117 269 552 1402 1518
Size: 117 152 283 850 116
Fragments arranged by size:
850 283 152 117 116
EcoRI G'AATT_C
Cuts at: 0 980 1518
Size: 980 538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 747 1518
Size: 747 771
FspI TGC'GCA
Cuts at: 0 133 1518
Size: 133 1385
FspAI rTGC'GCAy
Cuts at: 0 133 1518
Size: 133 1385
HaeII r_GCGC'y
Cuts at: 0 40 77 1351 1518
Size: 40 37 1274 167
Fragments arranged by size:
1274 167 40 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 1386 1418 1518
Size: 316 32 1038 32 100
Fragments arranged by size:
1038 316 100 32 32
HincII GTy'rAC
Cuts at: 0 729 1518
Size: 729 789
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1518
Size: 665 853
MslI CAynn'nnrTG
Cuts at: 0 85 403 1518
Size: 85 318 1115
Fragments arranged by size:
1115 318 85
MspA1I CmG'CkG
Cuts at: 0 1167 1236 1299 1438 1493 1518
Size: 1167 69 63 139 55 25
Fragments arranged by size:
1167 139 69 63 55 25
NcoI C'CATG_G
Cuts at: 0 1262 1518
Size: 1262 256
NspI r_CATG'y
Cuts at: 0 165 594 1518
Size: 165 429 924
Fragments arranged by size:
924 429 165
PciI A'CATG_T
Cuts at: 0 161 1518
Size: 161 1357
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1518
Size: 699 819
PfoI T'CCnGG_A
Cuts at: 0 1112 1478 1518
Size: 1112 366 40
Fragments arranged by size:
1112 366 40
PpuMI rG'GwC_Cy
Cuts at: 0 117 552 1402 1518
Size: 117 435 850 116
Fragments arranged by size:
850 435 117 116
PspOMI G'GGCC_C
Cuts at: 0 268 1518
Size: 268 1250
PspXI vC'TCGA_Gb
Cuts at: 0 340 1518
Size: 340 1178
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 271 303 1518
Size: 271 32 1215
Fragments arranged by size:
1215 271 32
PstI C_TGCA'G
Cuts at: 0 1003 1166 1518
Size: 1003 163 352
Fragments arranged by size:
1003 352 163
PvuII CAG'CTG
Cuts at: 0 1167 1299 1518
Size: 1167 132 219
Fragments arranged by size:
1167 219 132
SacI G_AGCT'C
Cuts at: 0 632 1518
Size: 632 886
SapI GCTCTTCn'nnn_
Cuts at: 0 658 1518
Size: 658 860
SbfI CC_TGCA'GG
Cuts at: 0 1003 1518
Size: 1003 515
ScaI AGT'ACT
Cuts at: 0 580 1518
Size: 580 938
SfcI C'TryA_G
Cuts at: 0 822 999 1162 1518
Size: 822 177 163 356
Fragments arranged by size:
822 356 177 163
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1449 1518
Size: 1449 69
SmlI C'TyrA_G
Cuts at: 0 121 340 1518
Size: 121 219 1178
Fragments arranged by size:
1178 219 121
StuI AGG'CCT
Cuts at: 0 835 1430 1518
Size: 835 595 88
Fragments arranged by size:
835 595 88
StyI C'CwwG_G
Cuts at: 0 479 1262 1313 1398 1518
Size: 479 783 51 85 120
Fragments arranged by size:
783 479 120 85 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 517 960 1518
Size: 415 102 443 558
Fragments arranged by size:
558 443 415 102
TatI w'GTAC_w
Cuts at: 0 278 578 857 1518
Size: 278 300 279 661
Fragments arranged by size:
661 300 279 278
XhoI C'TCGA_G
Cuts at: 0 340 1518
Size: 340 1178
ZraI GAC'GTC
Cuts at: 0 213 1518
Size: 213 1305
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BclI BglI
BlpI Bme1580I BmrI BplI BpmI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstXI
BstYI Bsu36I BtgI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII MmeI MslI MspA1I NcoI NspI PciI PflMI
PfoI PpuMI PspOMI PspXI PsrI PstI PvuII SacI
SapI SbfI ScaI SfcI SfiI SmlI StuI StyI
TaqII TatI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AleI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglII BmgBI BmtI Bpu10I BpuEI
BsaI BseRI BsiEI BsiWI BsmBI BsrDI BstAPI BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI EagI
EciI EcoNI EcoRV FseI HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PvuI RsrII SacII SalI SanDI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XmaI XmnI