Restriction map for
pFN21AE2791
(Linear) (Six-base) MAPSORT of: KIEE2791.seq Check: 447 from: 1 to: 3495
KIEE2791.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 191 941 1837 2476 3495
Size: 191 750 896 639 1019
Fragments arranged by size:
1019 896 750 639 191
AatII G_ACGT'C
Cuts at: 0 2618 3495
Size: 2618 877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 1012 1983 2253 3495
Size: 65 947 971 270 1242
Fragments arranged by size:
1242 971 947 270 65
AfeI AGC'GCT
Cuts at: 0 2989 3495
Size: 2989 506
AflIII A'CryG_T
Cuts at: 0 2939 2969 3495
Size: 2939 30 526
Fragments arranged by size:
2939 526 30
AleI CACnn'nnGTG
Cuts at: 0 2899 3440 3495
Size: 2899 541 55
Fragments arranged by size:
2899 541 55
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 2494 3495
Size: 1294 1200 1001
Fragments arranged by size:
1294 1200 1001
AlwNI CAG_nnn'CTG
Cuts at: 0 933 1907 2046 2679 3495
Size: 933 974 139 633 816
Fragments arranged by size:
974 933 816 633 139
ApaI G_GGCC'C
Cuts at: 0 514 1489 2290 2300 2581 3495
Size: 514 975 801 10 281 914
Fragments arranged by size:
975 914 801 514 281 10
ApoI r'AATT_y
Cuts at: 0 2176 3495
Size: 2176 1319
AvaI C'yCGr_G
Cuts at: 0 34 570 765 1177 1352 1703 2299 3274
Size: 34 536 195 412 175 351 596 975
Cuts at: 3274 3466 3495
Size: 192 29
Fragments arranged by size:
975 596 536 412 351 195 192 175
34 29
BamHI G'GATC_C
Cuts at: 0 305 3495
Size: 305 3190
BanI G'GyrC_C
Cuts at: 0 126 179 819 1339 2115 3495
Size: 126 53 640 520 776 1380
Fragments arranged by size:
1380 776 640 520 126 53
BanII G_rGCy'C
Cuts at: 0 514 967 1055 1253 1489 2074 2127 2290
Size: 514 453 88 198 236 585 53 163
Cuts at: 2290 2300 2581 3394 3495
Size: 10 281 813 101
Fragments arranged by size:
813 585 514 453 281 236 198 163
101 88 53 10
BbeI G_GCGC'C
Cuts at: 0 2119 3495
Size: 2119 1376
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 2145 2610 3072 3495
Size: 53 2092 465 462 423
Fragments arranged by size:
2092 465 462 423 53
BbvCI CC'TCA_GC
Cuts at: 0 604 3495
Size: 604 2891
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 769 803 2493 2527 3495
Size: 769 34 1690 34 968
Fragments arranged by size:
1690 968 769 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1645 1699 1849 3495
Size: 1645 54 150 1646
Fragments arranged by size:
1646 1645 150 54
BglII A'GATC_T
Cuts at: 0 1100 2747 3495
Size: 1100 1647 748
Fragments arranged by size:
1647 1100 748
BlpI GC'TnA_GC
Cuts at: 0 3049 3095 3495
Size: 3049 46 400
Fragments arranged by size:
3049 400 46
Bme1580I G_kGCm'C
Cuts at: 0 321 514 652 824 1342 1489 2290 2300
Size: 321 193 138 172 518 147 801 10
Cuts at: 2300 2581 3495
Size: 281 914
Fragments arranged by size:
914 801 518 321 281 193 172 147
138 10
BmgBI CAC'GTC
Cuts at: 0 383 1116 1215 3363 3495
Size: 383 733 99 2148 132
Fragments arranged by size:
2148 733 383 132 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 1978 3495
Size: 735 1243 1517
Fragments arranged by size:
1517 1243 735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 631 705 1159 1702 1836 2050 2359
Size: 472 159 74 454 543 134 214 309
Cuts at: 2359 2725 2839 3136 3334 3495
Size: 366 114 297 198 161
Fragments arranged by size:
543 472 454 366 309 297 214 198
161 159 134 114 74
Bpu10I CC'TnA_GC
Cuts at: 0 604 2126 3058 3187 3495
Size: 604 1522 932 129 308
Fragments arranged by size:
1522 932 604 308 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1393 3495
Size: 1039 354 2102
Fragments arranged by size:
2102 1039 354
BsaI GGTCTCn'nnnn_
Cuts at: 0 1401 3116 3495
Size: 1401 1715 379
Fragments arranged by size:
1715 1401 379
BsaAI yAC'GTr
Cuts at: 0 2146 3495
Size: 2146 1349
BsaBI GATnn'nnATC
Cuts at: 0 2551 3495
Size: 2551 944
BsaHI Gr'CG_yC
Cuts at: 0 1157 1427 1856 2116 2615 3495
Size: 1157 270 429 260 499 880
Fragments arranged by size:
1157 880 499 429 270 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1111 1141 1381 1411 1735 1765 3495
Size: 1111 30 240 30 324 30 1730
Fragments arranged by size:
1730 1111 324 240 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 469 985 1156 1324 2116 3222 3495
Size: 235 234 516 171 168 792 1106 273
Fragments arranged by size:
1106 792 516 273 235 234 171 168
BseYI C'CCAG_C
Cuts at: 0 60 423 859 1343 2574 2839 3455 3495
Size: 60 363 436 484 1231 265 616 40
Fragments arranged by size:
1231 616 484 436 363 265 60 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1372 1819 2668 2974 3495
Size: 421 951 447 849 306 521
Fragments arranged by size:
951 849 521 447 421 306
BsiEI CG_ry'CG
Cuts at: 0 1455 2700 3495
Size: 1455 1245 795
Fragments arranged by size:
1455 1245 795
BsiHKAI G_wGCw'C
Cuts at: 0 155 295 1055 1253 2729 3101 3113 3344
Size: 155 140 760 198 1476 372 12 231
Cuts at: 3344 3394 3495
Size: 50 101
Fragments arranged by size:
1476 760 372 231 198 155 140 101
50 12
BsmI GAATG_Cn'
Cuts at: 0 446 3495
Size: 446 3049
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1678 3495
Size: 1678 1817
Bsp1286I G_dGCh'C
Cuts at: 0 155 295 321 514 652 824 967 1055
Size: 155 140 26 193 138 172 143 88
Cuts at: 1055 1253 1342 1489 2074 2127 2290 2300 2581
Size: 198 89 147 585 53 163 10 281
Cuts at: 2581 2729 3101 3113 3344 3394 3495
Size: 148 372 12 231 50 101
Fragments arranged by size:
585 372 281 231 198 193 172 163
155 148 147 143 140 138 101 89
88 53 50 26 12 10
BspHI T'CATG_A
Cuts at: 0 2909 3495
Size: 2909 586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 191 941 1298 1837 2045 2476 2750
Size: 170 21 750 357 539 208 431 274
Cuts at: 2750 2924 3134 3495
Size: 174 210 361
Fragments arranged by size:
750 539 431 361 357 274 210 208
174 170 21
BsrBI CCG'CTC
Cuts at: 0 134 1259 1731 3495
Size: 134 1125 472 1764
Fragments arranged by size:
1764 1125 472 134
BsrDI GCAATG_nn'
Cuts at: 0 3260 3495
Size: 3260 235
BsrFI r'CCGG_y
Cuts at: 0 50 186 1330 1374 1454 2732 3495
Size: 50 136 1144 44 80 1278 763
Fragments arranged by size:
1278 1144 763 136 80 50 44
BssSI C'ACGA_G
Cuts at: 0 1390 1591 2650 3013 3343 3495
Size: 1390 201 1059 363 330 152
Fragments arranged by size:
1390 1059 363 330 201 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 357 3495
Size: 357 3138
BstEII G'GTnAC_C
Cuts at: 0 2131 2304 2877 2892 3495
Size: 2131 173 573 15 603
Fragments arranged by size:
2131 603 573 173 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 708 729 2859 3442 3495
Size: 201 507 21 2130 583 53
Fragments arranged by size:
2130 583 507 201 53 21
BstYI r'GATC_y
Cuts at: 0 305 665 716 1100 1904 2711 2747 2759
Size: 305 360 51 384 804 807 36 12
Cuts at: 2759 3495
Size: 736
Fragments arranged by size:
807 804 736 384 360 305 51 36
12
Bsu36I CC'TnA_GG
Cuts at: 0 488 1034 2017 2292 3495
Size: 488 546 983 275 1203
Fragments arranged by size:
1203 983 546 488 275
BtgI C'CryG_G
Cuts at: 0 728 745 1064 1364 1502 1616 2516 3353
Size: 728 17 319 300 138 114 900 837
Cuts at: 3353 3495
Size: 142
Fragments arranged by size:
900 837 728 319 300 142 138 114
17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 596 759 1071 2907 3495
Size: 596 163 312 1836 588
Fragments arranged by size:
1836 596 588 312 163
BtsI GCAGTG_nn'
Cuts at: 0 2809 3495
Size: 2809 686
DraIII CAC_nnn'GTG
Cuts at: 0 2385 3252 3495
Size: 2385 867 243
Fragments arranged by size:
2385 867 243
DrdI GACnn_nn'nnGTC
Cuts at: 0 1162 3148 3495
Size: 1162 1986 347
Fragments arranged by size:
1986 1162 347
EaeI y'GGCC_r
Cuts at: 0 335 374 830 1061 1217 1328 1452 1456
Size: 335 39 456 231 156 111 124 4
Cuts at: 1456 1490 1598 1646 1841 1922 2438 2499 2697
Size: 34 108 48 195 81 516 61 198
Cuts at: 2697 2754 3474 3495
Size: 57 720 21
Fragments arranged by size:
720 516 456 335 231 198 195 156
124 111 108 81 61 57 48 39
34 21 4
EagI C'GGCC_G
Cuts at: 0 1452 2697 3495
Size: 1452 1245 798
Fragments arranged by size:
1452 1245 798
EarI CTCTTCn'nnn_
Cuts at: 0 1288 1531 1753 2062 2242 2635 3495
Size: 1288 243 222 309 180 393 860
Fragments arranged by size:
1288 860 393 309 243 222 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1492 2187 2707 3061 3495
Size: 1492 695 520 354 434
Fragments arranged by size:
1492 695 520 434 354
EcoICRI GAG'CTC
Cuts at: 0 1053 1251 3392 3495
Size: 1053 198 2141 103
Fragments arranged by size:
2141 1053 198 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 472 631 705 1012 1159 1702 1836
Size: 65 407 159 74 307 147 543 134
Cuts at: 1836 1983 2050 2253 2359 2725 2839 3136 3334
Size: 147 67 203 106 366 114 297 198
Cuts at: 3334 3495
Size: 161
Fragments arranged by size:
543 407 366 307 297 203 198 161
159 147 147 134 114 106 74 67
65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 711 1141 1390 3495
Size: 711 430 249 2105
Fragments arranged by size:
2105 711 430 249
EcoO109I rG'GnC_Cy
Cuts at: 0 16 510 511 565 1485 1557 2034 2043
Size: 16 494 1 54 920 72 477 9
Cuts at: 2043 2286 2287 2296 2578 3495
Size: 243 1 9 282 917
Fragments arranged by size:
920 917 494 477 282 243 72 54
16 9 9 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1884 2634 3198 3495
Size: 1884 750 564 297
Fragments arranged by size:
1884 750 564 297
FseI GG_CCGG'CC
Cuts at: 0 1334 1458 3495
Size: 1334 124 2037
Fragments arranged by size:
2037 1334 124
FspI TGC'GCA
Cuts at: 0 265 3495
Size: 265 3230
HaeII r_GCGC'y
Cuts at: 0 1000 2119 2189 2680 2991 3021 3495
Size: 1000 1119 70 491 311 30 474
Fragments arranged by size:
1119 1000 491 474 311 70 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 290 322 1111 1143 1735 1767 2815 2847
Size: 290 32 789 32 592 32 1048 32
Cuts at: 2847 3495
Size: 648
Fragments arranged by size:
1048 789 648 592 290 32 32 32
32
HincII GTy'rAC
Cuts at: 0 84 3495
Size: 84 3411
KasI G'GCGC_C
Cuts at: 0 2115 3495
Size: 2115 1380
MscI TGG'CCA
Cuts at: 0 337 376 1600 1843 2440 3476 3495
Size: 337 39 1224 243 597 1036 19
Fragments arranged by size:
1224 1036 597 337 243 39 19
MslI CAynn'nnrTG
Cuts at: 0 553 2690 2899 2926 3440 3495
Size: 553 2137 209 27 514 55
Fragments arranged by size:
2137 553 514 209 55 27
MspA1I CmG'CkG
Cuts at: 0 1299 1366 1811 2076 2574 3420 3495
Size: 1299 67 445 265 498 846 75
Fragments arranged by size:
1299 846 498 445 265 75 67
NaeI GCC'GGC
Cuts at: 0 188 1332 1456 3495
Size: 188 1144 124 2039
Fragments arranged by size:
2039 1144 188 124
NarI GG'CG_CC
Cuts at: 0 2116 3495
Size: 2116 1379
NcoI C'CATG_G
Cuts at: 0 1616 2516 3495
Size: 1616 900 979
Fragments arranged by size:
1616 979 900
NgoMIV G'CCGG_C
Cuts at: 0 186 1330 1454 3495
Size: 186 1144 124 2041
Fragments arranged by size:
2041 1144 186 124
NspI r_CATG'y
Cuts at: 0 346 2973 3495
Size: 346 2627 522
Fragments arranged by size:
2627 522 346
PasI CC'CwG_GG
Cuts at: 0 2283 3372 3495
Size: 2283 1089 123
Fragments arranged by size:
2283 1089 123
PciI A'CATG_T
Cuts at: 0 2969 3495
Size: 2969 526
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 816 1596 1907 3252 3423 3495
Size: 372 444 780 311 1345 171 72
Fragments arranged by size:
1345 780 444 372 311 171 72
PfoI T'CCnGG_A
Cuts at: 0 24 3495
Size: 24 3471
PmlI CAC'GTG
Cuts at: 0 2146 3495
Size: 2146 1349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 3495
Size: 904 32 2559
Fragments arranged by size:
2559 904 32
PpuMI rG'GwC_Cy
Cuts at: 0 1557 2034 2043 3495
Size: 1557 477 9 1452
Fragments arranged by size:
1557 1452 477 9
PspOMI G'GGCC_C
Cuts at: 0 510 1485 2286 2296 2577 3495
Size: 510 975 801 10 281 918
Fragments arranged by size:
975 918 801 510 281 10
PstI C_TGCA'G
Cuts at: 0 911 1247 1298 1424 1583 2042 2090 2473
Size: 911 336 51 126 159 459 48 383
Cuts at: 2473 2747 3495
Size: 274 748
Fragments arranged by size:
911 748 459 383 336 274 159 126
51 48
PvuII CAG'CTG
Cuts at: 0 2574 3420 3495
Size: 2574 846 75
Fragments arranged by size:
2574 846 75
SacI G_AGCT'C
Cuts at: 0 1055 1253 3394 3495
Size: 1055 198 2141 101
Fragments arranged by size:
2141 1055 198 101
SacII CC_GC'GG
Cuts at: 0 1367 3495
Size: 1367 2128
SapI GCTCTTCn'nnn_
Cuts at: 0 1288 1531 2062 3495
Size: 1288 243 531 1433
Fragments arranged by size:
1433 1288 531 243
SbfI CC_TGCA'GG
Cuts at: 0 1424 2042 2473 3495
Size: 1424 618 431 1022
Fragments arranged by size:
1424 1022 618 431
SexAI A'CCwGG_T
Cuts at: 0 197 2381 3495
Size: 197 2184 1114
Fragments arranged by size:
2184 1114 197
SfcI C'TryA_G
Cuts at: 0 907 1243 1294 1420 1579 1761 2038 2086
Size: 907 336 51 126 159 182 277 48
Cuts at: 2086 2469 2743 3495
Size: 383 274 752
Fragments arranged by size:
907 752 383 336 277 274 182 159
126 51 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1699 3495
Size: 1699 1796
SfoI GGC'GCC
Cuts at: 0 2117 3495
Size: 2117 1378
SmaI CCC'GGG
Cuts at: 0 36 572 1354 3468 3495
Size: 36 536 782 2114 27
Fragments arranged by size:
2114 782 536 36 27
SmlI C'TyrA_G
Cuts at: 0 1054 1408 3495
Size: 1054 354 2087
Fragments arranged by size:
2087 1054 354
StuI AGG'CCT
Cuts at: 0 1864 1891 2027 3495
Size: 1864 27 136 1468
Fragments arranged by size:
1864 1468 136 27
StyI C'CwwG_G
Cuts at: 0 12 389 707 824 1616 1859 2516 3495
Size: 12 377 318 117 792 243 657 979
Fragments arranged by size:
979 792 657 377 318 243 117 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 205 1357 2150 2449 2686 2818 2822 3495
Size: 205 1152 793 299 237 132 4 673
Fragments arranged by size:
1152 793 673 299 237 205 132 4
TatI w'GTAC_w
Cuts at: 0 2222 3495
Size: 2222 1273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1105 1116 1137 1148 1381 1413 2412 2444
Size: 1105 11 21 11 233 32 999 32
Cuts at: 2444 3495
Size: 1051
Fragments arranged by size:
1105 1051 999 233 32 32 21 11
11
Tth111I GACn'n_nGTC
Cuts at: 0 982 3495
Size: 982 2513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 2523 2589 3495
Size: 396 2127 66 906
Fragments arranged by size:
2127 906 396 66
XmaI C'CCGG_G
Cuts at: 0 34 570 1352 3466 3495
Size: 34 536 782 2114 29
Fragments arranged by size:
2114 782 536 34 29
XmnI GAAnn'nnTTC
Cuts at: 0 2392 3495
Size: 2392 1103
ZraI GAC'GTC
Cuts at: 0 2616 3495
Size: 2616 879
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI FspI HaeII Hin4I HincII KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI
BmrI BmtI BsaWI BsiWI BspEI BsrGI BssHII BstBI
BstZ17I ClaI CspCI DraI EcoRI EcoRV FspAI HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI ScaI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI