Restriction map for
pFN21AE2787
(Linear) (Six-base) MAPSORT of: KIEE2787.seq Check: 8981 from: 1 to: 1731
KIEE2787.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1350 1731
Size: 1350 381
AccI GT'mk_AC
Cuts at: 0 1328 1731
Size: 1328 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1225 1731
Size: 1168 57 506
Fragments arranged by size:
1168 506 57
AflIII A'CryG_T
Cuts at: 0 776 1731
Size: 776 955
ApoI r'AATT_y
Cuts at: 0 1058 1259 1420 1731
Size: 1058 201 161 311
Fragments arranged by size:
1058 311 201 161
AseI AT'TA_AT
Cuts at: 0 1304 1731
Size: 1304 427
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 778 811 1731
Size: 778 33 920
Fragments arranged by size:
920 778 33
BanI G'GyrC_C
Cuts at: 0 33 192 426 535 1362 1731
Size: 33 159 234 109 827 369
Fragments arranged by size:
827 369 234 159 109 33
BanII G_rGCy'C
Cuts at: 0 10 265 1731
Size: 10 255 1466
Fragments arranged by size:
1466 255 10
BbeI G_GCGC'C
Cuts at: 0 37 196 430 539 1731
Size: 37 159 234 109 1192
Fragments arranged by size:
1192 234 159 109 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 1030 1185 1731
Size: 708 322 155 546
Fragments arranged by size:
708 546 322 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1381 1415 1731
Size: 1381 34 316
Fragments arranged by size:
1381 316 34
BglI GCCn_nnn'nGGC
Cuts at: 0 73 226 1731
Size: 73 153 1505
Fragments arranged by size:
1505 153 73
BglII A'GATC_T
Cuts at: 0 1394 1731
Size: 1394 337
BlpI GC'TnA_GC
Cuts at: 0 322 1731
Size: 322 1409
Bme1580I G_kGCm'C
Cuts at: 0 1715 1731
Size: 1715 16
BmgBI CAC'GTC
Cuts at: 0 164 1731
Size: 164 1567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 1654 1731
Size: 409 1245 77
Fragments arranged by size:
1245 409 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1731
Size: 157 1574
BsaI GGTCTCn'nnnn_
Cuts at: 0 1676 1731
Size: 1676 55
BsaBI GATnn'nnATC
Cuts at: 0 1384 1731
Size: 1384 347
BsaHI Gr'CG_yC
Cuts at: 0 34 193 427 440 536 1490 1731
Size: 34 159 234 13 96 954 241
Fragments arranged by size:
954 241 234 159 96 34 13
BsaWI w'CCGG_w
Cuts at: 0 561 1731
Size: 561 1170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 735 765 1062 1092 1731
Size: 735 30 297 30 639
Fragments arranged by size:
735 639 297 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1070 1731
Size: 1070 661
BseYI C'CCAG_C
Cuts at: 0 42 79 203 297 342 1731
Size: 42 37 124 94 45 1389
Fragments arranged by size:
1389 124 94 45 42 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1731
Size: 405 1326
BsiEI CG_ry'CG
Cuts at: 0 68 1731
Size: 68 1663
BsiHKAI G_wGCw'C
Cuts at: 0 1127 1243 1731
Size: 1127 116 488
Fragments arranged by size:
1127 488 116
Bsp1286I G_dGCh'C
Cuts at: 0 10 265 1127 1243 1715 1731
Size: 10 255 862 116 472 16
Fragments arranged by size:
862 472 255 116 16 10
BspEI T'CCGG_A
Cuts at: 0 561 1731
Size: 561 1170
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 1350 1731
Size: 347 1003 381
Fragments arranged by size:
1003 381 347
BsrBI CCG'CTC
Cuts at: 0 30 1731
Size: 30 1701
BsrDI GCAATG_nn'
Cuts at: 0 635 1731
Size: 635 1096
BsrFI r'CCGG_y
Cuts at: 0 95 462 1731
Size: 95 367 1269
Fragments arranged by size:
1269 367 95
BssHII G'CGCG_C
Cuts at: 0 47 99 141 212 307 309 406 1731
Size: 47 52 42 71 95 2 97 1325
Fragments arranged by size:
1325 97 95 71 52 47 42 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1374 1731
Size: 1374 357
BstXI CCAn_nnnn'nTGG
Cuts at: 0 838 1731
Size: 838 893
BstYI r'GATC_y
Cuts at: 0 1394 1731
Size: 1394 337
BtgI C'CryG_G
Cuts at: 0 68 1217 1731
Size: 68 1149 514
Fragments arranged by size:
1149 514 68
BtsI GCAGTG_nn'
Cuts at: 0 768 1731
Size: 768 963
DrdI GACnn_nn'nnGTC
Cuts at: 0 1159 1731
Size: 1159 572
EaeI y'GGCC_r
Cuts at: 0 65 284 368 464 503 726 1731
Size: 65 219 84 96 39 223 1005
Fragments arranged by size:
1005 223 219 96 84 65 39
EagI C'GGCC_G
Cuts at: 0 65 1731
Size: 65 1666
EarI CTCTTCn'nnn_
Cuts at: 0 244 920 1201 1731
Size: 244 676 281 530
Fragments arranged by size:
676 530 281 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1168 1225 1731
Size: 157 1011 57 506
Fragments arranged by size:
1011 506 157 57
HaeII r_GCGC'y
Cuts at: 0 37 196 232 334 430 539 831 1731
Size: 37 159 36 102 96 109 292 900
Fragments arranged by size:
900 292 159 109 102 96 37 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1542 1574 1731
Size: 1542 32 157
Fragments arranged by size:
1542 157 32
KasI G'GCGC_C
Cuts at: 0 33 192 426 535 1731
Size: 33 159 234 109 1196
Fragments arranged by size:
1196 234 159 109 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1731
Size: 191 1540
MscI TGG'CCA
Cuts at: 0 505 728 1731
Size: 505 223 1003
Fragments arranged by size:
1003 505 223
MslI CAynn'nnrTG
Cuts at: 0 775 1731
Size: 775 956
MspA1I CmG'CkG
Cuts at: 0 39 70 363 452 1435 1731
Size: 39 31 293 89 983 296
Fragments arranged by size:
983 296 293 89 39 31
NaeI GCC'GGC
Cuts at: 0 97 1731
Size: 97 1634
NarI GG'CG_CC
Cuts at: 0 34 193 427 536 1731
Size: 34 159 234 109 1195
Fragments arranged by size:
1195 234 159 109 34
NdeI CA'TA_TG
Cuts at: 0 1023 1731
Size: 1023 708
NgoMIV G'CCGG_C
Cuts at: 0 95 1731
Size: 95 1636
NotI GC'GGCC_GC
Cuts at: 0 65 1731
Size: 65 1666
NspI r_CATG'y
Cuts at: 0 351 780 1227 1731
Size: 351 429 447 504
Fragments arranged by size:
504 447 429 351
PciI A'CATG_T
Cuts at: 0 776 1731
Size: 776 955
PflMI CCAn_nnn'nTGG
Cuts at: 0 1547 1731
Size: 1547 184
PstI C_TGCA'G
Cuts at: 0 1098 1257 1731
Size: 1098 159 474
Fragments arranged by size:
1098 474 159
PvuII CAG'CTG
Cuts at: 0 1435 1731
Size: 1435 296
SacII CC_GC'GG
Cuts at: 0 71 1731
Size: 71 1660
SapI GCTCTTCn'nnn_
Cuts at: 0 244 1731
Size: 244 1487
ScaI AGT'ACT
Cuts at: 0 1372 1731
Size: 1372 359
SexAI A'CCwGG_T
Cuts at: 0 170 542 1731
Size: 170 372 1189
Fragments arranged by size:
1189 372 170
SfcI C'TryA_G
Cuts at: 0 1094 1253 1731
Size: 1094 159 478
Fragments arranged by size:
1094 478 159
SfoI GGC'GCC
Cuts at: 0 35 194 428 537 1731
Size: 35 159 234 109 1194
Fragments arranged by size:
1194 234 159 109 35
SphI G_CATG'C
Cuts at: 0 351 1731
Size: 351 1380
SspI AAT'ATT
Cuts at: 0 1068 1578 1731
Size: 1068 510 153
Fragments arranged by size:
1068 510 153
StuI AGG'CCT
Cuts at: 0 1415 1731
Size: 1415 316
TatI w'GTAC_w
Cuts at: 0 1370 1731
Size: 1370 361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 735 767 1731
Size: 735 32 964
Fragments arranged by size:
964 735 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 817 1731
Size: 817 914
Enzymes that do cut:
AarI AccI AcuI AflIII ApoI AseI BaeI BanI
BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I
BmgBI BplI BpmI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI BtgI
BtsI DrdI EaeI EagI EarI Eco57MI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NotI NspI PciI PflMI PstI PvuII SacII
SapI ScaI SexAI SfcI SfoI SphI SspI StuI
TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BamHI BbvCI BciVI BclI BfrBI BmrI BmtI
Bpu10I BpuEI BsaAI BsiWI BsmI BsmBI BspHI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NheI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI
ZraI