Restriction map for
pFN21AE2779
(Linear) (Six-base) MAPSORT of: KIEE2779.seq Check: 6866 from: 1 to: 2427
KIEE2779.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
Acc65I G'GTAC_C
Cuts at: 0 700 2427
Size: 700 1727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 2102 2266 2326 2427
Size: 153 1949 164 60 101
Fragments arranged by size:
1949 164 153 101 60
AfeI AGC'GCT
Cuts at: 0 343 1522 2427
Size: 343 1179 905
Fragments arranged by size:
1179 905 343
AleI CACnn'nnGTG
Cuts at: 0 2356 2427
Size: 2356 71
AlwNI CAG_nnn'CTG
Cuts at: 0 2191 2427
Size: 2191 236
ApoI r'AATT_y
Cuts at: 0 1407 1649 2047 2284 2427
Size: 1407 242 398 237 143
Fragments arranged by size:
1407 398 242 237 143
AseI AT'TA_AT
Cuts at: 0 509 1279 1939 2427
Size: 509 770 660 488
Fragments arranged by size:
770 660 509 488
BamHI G'GATC_C
Cuts at: 0 388 2427
Size: 388 2039
BanI G'GyrC_C
Cuts at: 0 151 700 1218 2427
Size: 151 549 518 1209
Fragments arranged by size:
1209 549 518 151
BanII G_rGCy'C
Cuts at: 0 1571 2427
Size: 1571 856
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 2406 2427
Size: 1368 1038 21
Fragments arranged by size:
1368 1038 21
BbvCI CC'TCA_GC
Cuts at: 0 1537 2427
Size: 1537 890
BclI T'GATC_A
Cuts at: 0 1083 1911 2427
Size: 1083 828 516
Fragments arranged by size:
1083 828 516
BfrBI ATG'CAT
Cuts at: 0 1234 1415 2427
Size: 1234 181 1012
Fragments arranged by size:
1234 1012 181
BglII A'GATC_T
Cuts at: 0 80 2427
Size: 80 2347
Bme1580I G_kGCm'C
Cuts at: 0 135 154 2427
Size: 135 19 2273
Fragments arranged by size:
2273 135 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1604 2240 2427
Size: 910 694 636 187
Fragments arranged by size:
910 694 636 187
Bpu10I CC'TnA_GC
Cuts at: 0 1537 2427
Size: 1537 890
BsaHI Gr'CG_yC
Cuts at: 0 353 2427
Size: 353 2074
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 941 971 1431 1461 2126 2156
Size: 866 30 45 30 460 30 665 30
Cuts at: 2156 2427
Size: 271
Fragments arranged by size:
866 665 460 271 45 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 965 1870 2427
Size: 385 580 905 557
Fragments arranged by size:
905 580 557 385
BseYI C'CCAG_C
Cuts at: 0 276 2427
Size: 276 2151
BsiHKAI G_wGCw'C
Cuts at: 0 627 1571 2427
Size: 627 944 856
Fragments arranged by size:
944 856 627
BsmI GAATG_Cn'
Cuts at: 0 492 2427
Size: 492 1935
BsmBI CGTCTCn'nnnn_
Cuts at: 0 788 958 2427
Size: 788 170 1469
Fragments arranged by size:
1469 788 170
Bsp1286I G_dGCh'C
Cuts at: 0 135 154 627 1571 2427
Size: 135 19 473 944 856
Fragments arranged by size:
944 856 473 135 19
BspHI T'CATG_A
Cuts at: 0 1841 2427
Size: 1841 586
BssSI C'ACGA_G
Cuts at: 0 848 2165 2427
Size: 848 1317 262
Fragments arranged by size:
1317 848 262
BstEII G'GTnAC_C
Cuts at: 0 2268 2277 2427
Size: 2268 9 150
Fragments arranged by size:
2268 150 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 2427
Size: 304 2123
BstYI r'GATC_y
Cuts at: 0 80 388 1155 2392 2427
Size: 80 308 767 1237 35
Fragments arranged by size:
1237 767 308 80 35
BtgI C'CryG_G
Cuts at: 0 636 2091 2427
Size: 636 1455 336
Fragments arranged by size:
1455 636 336
DraI TTT'AAA
Cuts at: 0 1754 2427
Size: 1754 673
DraIII CAC_nnn'GTG
Cuts at: 0 853 2427
Size: 853 1574
EarI CTCTTCn'nnn_
Cuts at: 0 786 1679 2258 2427
Size: 786 893 579 169
Fragments arranged by size:
893 786 579 169
EcoICRI GAG'CTC
Cuts at: 0 1569 2427
Size: 1569 858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 910 1604 2102 2240 2266 2326 2427
Size: 153 757 694 498 138 26 60 101
Fragments arranged by size:
757 694 498 153 138 101 60 26
EcoO109I rG'GnC_Cy
Cuts at: 0 783 1089 2188 2427
Size: 783 306 1099 239
Fragments arranged by size:
1099 783 306 239
EcoRI G'AATT_C
Cuts at: 0 2047 2427
Size: 2047 380
EcoRV GAT'ATC
Cuts at: 0 444 2427
Size: 444 1983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 408 754 1333 2427
Size: 183 225 346 579 1094
Fragments arranged by size:
1094 579 346 225 183
HaeII r_GCGC'y
Cuts at: 0 345 1269 1524 2427
Size: 345 924 255 903
Fragments arranged by size:
924 903 345 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 727 759 941 973 2427
Size: 72 32 623 32 182 32 1454
Fragments arranged by size:
1454 623 182 72 32 32 32
HincII GTy'rAC
Cuts at: 0 48 524 2427
Size: 48 476 1903
Fragments arranged by size:
1903 476 48
HpaI GTT'AAC
Cuts at: 0 48 2427
Size: 48 2379
KpnI G_GTAC'C
Cuts at: 0 704 2427
Size: 704 1723
MfeI C'AATT_G
Cuts at: 0 983 2427
Size: 983 1444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1884 2427
Size: 1884 543
MslI CAynn'nnrTG
Cuts at: 0 302 2356 2427
Size: 302 2054 71
Fragments arranged by size:
2054 302 71
MspA1I CmG'CkG
Cuts at: 0 757 1393 2427
Size: 757 636 1034
Fragments arranged by size:
1034 757 636
NcoI C'CATG_G
Cuts at: 0 636 2091 2427
Size: 636 1455 336
Fragments arranged by size:
1455 636 336
NsiI A_TGCA'T
Cuts at: 0 1236 1417 2427
Size: 1236 181 1010
Fragments arranged by size:
1236 1010 181
PpuMI rG'GwC_Cy
Cuts at: 0 1089 2188 2427
Size: 1089 1099 239
Fragments arranged by size:
1099 1089 239
PstI C_TGCA'G
Cuts at: 0 1289 1557 2427
Size: 1289 268 870
Fragments arranged by size:
1289 870 268
PvuII CAG'CTG
Cuts at: 0 757 1393 2427
Size: 757 636 1034
Fragments arranged by size:
1034 757 636
SacI G_AGCT'C
Cuts at: 0 1571 2427
Size: 1571 856
SapI GCTCTTCn'nnn_
Cuts at: 0 1679 2427
Size: 1679 748
SexAI A'CCwGG_T
Cuts at: 0 1734 2427
Size: 1734 693
SfcI C'TryA_G
Cuts at: 0 1285 1553 2004 2427
Size: 1285 268 451 423
Fragments arranged by size:
1285 451 423 268
SpeI A'CTAG_T
Cuts at: 0 1633 2427
Size: 1633 794
StyI C'CwwG_G
Cuts at: 0 114 527 636 786 2091 2273 2427
Size: 114 413 109 150 1305 182 154
Fragments arranged by size:
1305 413 182 154 150 114 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 866 871 898 1431 1463 2126 2158
Size: 839 27 5 27 533 32 663 32
Cuts at: 2158 2427
Size: 269
Fragments arranged by size:
839 663 533 269 32 32 27 27
5
XbaI T'CTAG_A
Cuts at: 0 2395 2427
Size: 2395 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 598 2427
Size: 598 1829
XmnI GAAnn'nnTTC
Cuts at: 0 166 2427
Size: 166 2261
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI
DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI
MslI MspA1I NcoI NsiI PpuMI PstI PvuII SacI
SapI SexAI SfcI SpeI StyI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DrdI EaeI EagI EciI EcoNI FseI FspI
FspAI HindIII KasI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XhoI XmaI ZraI