Restriction map for
pFN21AE2778
(Linear) (Six-base) MAPSORT of: KIEE2778.seq Check: 1150 from: 1 to: 2343
KIEE2778.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
Acc65I G'GTAC_C
Cuts at: 0 700 2343
Size: 700 1643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 2102 2266 2343
Size: 153 1949 164 77
Fragments arranged by size:
1949 164 153 77
AfeI AGC'GCT
Cuts at: 0 343 1522 2343
Size: 343 1179 821
Fragments arranged by size:
1179 821 343
AleI CACnn'nnGTG
Cuts at: 0 2272 2343
Size: 2272 71
AlwNI CAG_nnn'CTG
Cuts at: 0 2191 2343
Size: 2191 152
ApoI r'AATT_y
Cuts at: 0 1407 1649 2047 2343
Size: 1407 242 398 296
Fragments arranged by size:
1407 398 296 242
AseI AT'TA_AT
Cuts at: 0 509 1279 1939 2343
Size: 509 770 660 404
Fragments arranged by size:
770 660 509 404
BamHI G'GATC_C
Cuts at: 0 388 2343
Size: 388 1955
BanI G'GyrC_C
Cuts at: 0 151 700 1218 2343
Size: 151 549 518 1125
Fragments arranged by size:
1125 549 518 151
BanII G_rGCy'C
Cuts at: 0 1571 2343
Size: 1571 772
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 2270 2322 2343
Size: 1368 902 52 21
Fragments arranged by size:
1368 902 52 21
BbvCI CC'TCA_GC
Cuts at: 0 1537 2343
Size: 1537 806
BclI T'GATC_A
Cuts at: 0 1083 1911 2343
Size: 1083 828 432
Fragments arranged by size:
1083 828 432
BfrBI ATG'CAT
Cuts at: 0 1234 1415 2343
Size: 1234 181 928
Fragments arranged by size:
1234 928 181
BglII A'GATC_T
Cuts at: 0 80 2343
Size: 80 2263
Bme1580I G_kGCm'C
Cuts at: 0 135 154 2343
Size: 135 19 2189
Fragments arranged by size:
2189 135 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1604 2240 2343
Size: 910 694 636 103
Fragments arranged by size:
910 694 636 103
Bpu10I CC'TnA_GC
Cuts at: 0 1537 2343
Size: 1537 806
BsaHI Gr'CG_yC
Cuts at: 0 353 2343
Size: 353 1990
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 941 971 1431 1461 2126 2156
Size: 866 30 45 30 460 30 665 30
Cuts at: 2156 2343
Size: 187
Fragments arranged by size:
866 665 460 187 45 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 965 1870 2343
Size: 385 580 905 473
Fragments arranged by size:
905 580 473 385
BseYI C'CCAG_C
Cuts at: 0 276 2343
Size: 276 2067
BsiHKAI G_wGCw'C
Cuts at: 0 627 1571 2343
Size: 627 944 772
Fragments arranged by size:
944 772 627
BsmI GAATG_Cn'
Cuts at: 0 492 2343
Size: 492 1851
BsmBI CGTCTCn'nnnn_
Cuts at: 0 788 958 2343
Size: 788 170 1385
Fragments arranged by size:
1385 788 170
Bsp1286I G_dGCh'C
Cuts at: 0 135 154 627 1571 2343
Size: 135 19 473 944 772
Fragments arranged by size:
944 772 473 135 19
BspHI T'CATG_A
Cuts at: 0 1841 2343
Size: 1841 502
BssSI C'ACGA_G
Cuts at: 0 848 2165 2343
Size: 848 1317 178
Fragments arranged by size:
1317 848 178
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 2343
Size: 304 2039
BstYI r'GATC_y
Cuts at: 0 80 388 1155 2308 2343
Size: 80 308 767 1153 35
Fragments arranged by size:
1153 767 308 80 35
BtgI C'CryG_G
Cuts at: 0 636 2091 2343
Size: 636 1455 252
Fragments arranged by size:
1455 636 252
DraI TTT'AAA
Cuts at: 0 1754 2343
Size: 1754 589
DraIII CAC_nnn'GTG
Cuts at: 0 853 2343
Size: 853 1490
EarI CTCTTCn'nnn_
Cuts at: 0 786 1679 2343
Size: 786 893 664
Fragments arranged by size:
893 786 664
EcoICRI GAG'CTC
Cuts at: 0 1569 2343
Size: 1569 774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 910 1604 2102 2240 2266 2343
Size: 153 757 694 498 138 26 77
Fragments arranged by size:
757 694 498 153 138 77 26
EcoO109I rG'GnC_Cy
Cuts at: 0 783 1089 2188 2343
Size: 783 306 1099 155
Fragments arranged by size:
1099 783 306 155
EcoRI G'AATT_C
Cuts at: 0 2047 2343
Size: 2047 296
EcoRV GAT'ATC
Cuts at: 0 444 2343
Size: 444 1899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 408 754 1333 2343
Size: 183 225 346 579 1010
Fragments arranged by size:
1010 579 346 225 183
HaeII r_GCGC'y
Cuts at: 0 345 1269 1524 2343
Size: 345 924 255 819
Fragments arranged by size:
924 819 345 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 727 759 941 973 2343
Size: 72 32 623 32 182 32 1370
Fragments arranged by size:
1370 623 182 72 32 32 32
HincII GTy'rAC
Cuts at: 0 48 524 2343
Size: 48 476 1819
Fragments arranged by size:
1819 476 48
HpaI GTT'AAC
Cuts at: 0 48 2343
Size: 48 2295
KpnI G_GTAC'C
Cuts at: 0 704 2343
Size: 704 1639
MfeI C'AATT_G
Cuts at: 0 983 2343
Size: 983 1360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1884 2343
Size: 1884 459
MslI CAynn'nnrTG
Cuts at: 0 302 2272 2343
Size: 302 1970 71
Fragments arranged by size:
1970 302 71
MspA1I CmG'CkG
Cuts at: 0 757 1393 2343
Size: 757 636 950
Fragments arranged by size:
950 757 636
NcoI C'CATG_G
Cuts at: 0 636 2091 2343
Size: 636 1455 252
Fragments arranged by size:
1455 636 252
NsiI A_TGCA'T
Cuts at: 0 1236 1417 2343
Size: 1236 181 926
Fragments arranged by size:
1236 926 181
PpuMI rG'GwC_Cy
Cuts at: 0 1089 2188 2343
Size: 1089 1099 155
Fragments arranged by size:
1099 1089 155
PstI C_TGCA'G
Cuts at: 0 1289 1557 2343
Size: 1289 268 786
Fragments arranged by size:
1289 786 268
PvuII CAG'CTG
Cuts at: 0 757 1393 2343
Size: 757 636 950
Fragments arranged by size:
950 757 636
SacI G_AGCT'C
Cuts at: 0 1571 2343
Size: 1571 772
SapI GCTCTTCn'nnn_
Cuts at: 0 1679 2343
Size: 1679 664
SexAI A'CCwGG_T
Cuts at: 0 1734 2343
Size: 1734 609
SfcI C'TryA_G
Cuts at: 0 1285 1553 2004 2343
Size: 1285 268 451 339
Fragments arranged by size:
1285 451 339 268
SpeI A'CTAG_T
Cuts at: 0 1633 2343
Size: 1633 710
StyI C'CwwG_G
Cuts at: 0 114 527 636 786 2091 2343
Size: 114 413 109 150 1305 252
Fragments arranged by size:
1305 413 252 150 114 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 866 871 898 1431 1463 2126 2158
Size: 839 27 5 27 533 32 663 32
Cuts at: 2158 2343
Size: 185
Fragments arranged by size:
839 663 533 185 32 32 27 27
5
XbaI T'CTAG_A
Cuts at: 0 2311 2343
Size: 2311 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 598 2343
Size: 598 1745
XmnI GAAnn'nnTTC
Cuts at: 0 166 2343
Size: 166 2177
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BssSI BstXI BstYI BtgI DraI
DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI
MspA1I NcoI NsiI PpuMI PstI PvuII SacI SapI
SexAI SfcI SpeI StyI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI
FspI FspAI HindIII KasI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XhoI XmaI ZraI