Restriction map for
pFN21AE2777
(Linear) (Six-base) MAPSORT of: KIEE2777.seq Check: 1594 from: 1 to: 2508
KIEE2777.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
Acc65I G'GTAC_C
Cuts at: 0 700 2508
Size: 700 1808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 2050 2105 2183 2347 2407 2508
Size: 153 1897 55 78 164 60 101
Fragments arranged by size:
1897 164 153 101 78 60 55
AfeI AGC'GCT
Cuts at: 0 343 1522 2508
Size: 343 1179 986
Fragments arranged by size:
1179 986 343
AleI CACnn'nnGTG
Cuts at: 0 2437 2508
Size: 2437 71
AlwNI CAG_nnn'CTG
Cuts at: 0 2272 2508
Size: 2272 236
ApoI r'AATT_y
Cuts at: 0 1407 1649 2128 2365 2508
Size: 1407 242 479 237 143
Fragments arranged by size:
1407 479 242 237 143
AseI AT'TA_AT
Cuts at: 0 509 1279 1939 2508
Size: 509 770 660 569
Fragments arranged by size:
770 660 569 509
BamHI G'GATC_C
Cuts at: 0 388 2508
Size: 388 2120
BanI G'GyrC_C
Cuts at: 0 151 700 1218 2508
Size: 151 549 518 1290
Fragments arranged by size:
1290 549 518 151
BanII G_rGCy'C
Cuts at: 0 1571 2508
Size: 1571 937
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 2056 2487 2508
Size: 1368 688 431 21
Fragments arranged by size:
1368 688 431 21
BbvCI CC'TCA_GC
Cuts at: 0 1537 2508
Size: 1537 971
BclI T'GATC_A
Cuts at: 0 1083 1911 2508
Size: 1083 828 597
Fragments arranged by size:
1083 828 597
BfrBI ATG'CAT
Cuts at: 0 1234 1415 2508
Size: 1234 181 1093
Fragments arranged by size:
1234 1093 181
BglII A'GATC_T
Cuts at: 0 80 2508
Size: 80 2428
Bme1580I G_kGCm'C
Cuts at: 0 135 154 2508
Size: 135 19 2354
Fragments arranged by size:
2354 135 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1604 2321 2508
Size: 910 694 717 187
Fragments arranged by size:
910 717 694 187
Bpu10I CC'TnA_GC
Cuts at: 0 1537 2508
Size: 1537 971
BsaHI Gr'CG_yC
Cuts at: 0 353 2508
Size: 353 2155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 941 971 1431 1461 2207 2237
Size: 866 30 45 30 460 30 746 30
Cuts at: 2237 2508
Size: 271
Fragments arranged by size:
866 746 460 271 45 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 965 1870 2508
Size: 385 580 905 638
Fragments arranged by size:
905 638 580 385
BseYI C'CCAG_C
Cuts at: 0 276 2508
Size: 276 2232
BsiHKAI G_wGCw'C
Cuts at: 0 627 1571 2508
Size: 627 944 937
Fragments arranged by size:
944 937 627
BsmI GAATG_Cn'
Cuts at: 0 492 2508
Size: 492 2016
BsmBI CGTCTCn'nnnn_
Cuts at: 0 788 958 2508
Size: 788 170 1550
Fragments arranged by size:
1550 788 170
Bsp1286I G_dGCh'C
Cuts at: 0 135 154 627 1571 2508
Size: 135 19 473 944 937
Fragments arranged by size:
944 937 473 135 19
BspHI T'CATG_A
Cuts at: 0 1841 2508
Size: 1841 667
BssSI C'ACGA_G
Cuts at: 0 848 2246 2508
Size: 848 1398 262
Fragments arranged by size:
1398 848 262
BstEII G'GTnAC_C
Cuts at: 0 2349 2358 2508
Size: 2349 9 150
Fragments arranged by size:
2349 150 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 2031 2508
Size: 304 1727 477
Fragments arranged by size:
1727 477 304
BstYI r'GATC_y
Cuts at: 0 80 388 1155 2473 2508
Size: 80 308 767 1318 35
Fragments arranged by size:
1318 767 308 80 35
BtgI C'CryG_G
Cuts at: 0 636 2172 2508
Size: 636 1536 336
Fragments arranged by size:
1536 636 336
DraI TTT'AAA
Cuts at: 0 1754 2508
Size: 1754 754
DraIII CAC_nnn'GTG
Cuts at: 0 853 2508
Size: 853 1655
EarI CTCTTCn'nnn_
Cuts at: 0 786 1679 2339 2508
Size: 786 893 660 169
Fragments arranged by size:
893 786 660 169
EcoICRI GAG'CTC
Cuts at: 0 1569 2508
Size: 1569 939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 910 1604 2050 2105 2183 2321 2347
Size: 153 757 694 446 55 78 138 26
Cuts at: 2347 2407 2508
Size: 60 101
Fragments arranged by size:
757 694 446 153 138 101 78 60
55 26
EcoO109I rG'GnC_Cy
Cuts at: 0 783 1089 2089 2269 2508
Size: 783 306 1000 180 239
Fragments arranged by size:
1000 783 306 239 180
EcoRI G'AATT_C
Cuts at: 0 2128 2508
Size: 2128 380
EcoRV GAT'ATC
Cuts at: 0 444 2508
Size: 444 2064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 408 754 1333 2080 2508
Size: 183 225 346 579 747 428
Fragments arranged by size:
747 579 428 346 225 183
HaeII r_GCGC'y
Cuts at: 0 345 1269 1524 2508
Size: 345 924 255 984
Fragments arranged by size:
984 924 345 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 727 759 941 973 2508
Size: 72 32 623 32 182 32 1535
Fragments arranged by size:
1535 623 182 72 32 32 32
HincII GTy'rAC
Cuts at: 0 48 524 2508
Size: 48 476 1984
Fragments arranged by size:
1984 476 48
HpaI GTT'AAC
Cuts at: 0 48 2508
Size: 48 2460
KpnI G_GTAC'C
Cuts at: 0 704 2508
Size: 704 1804
MfeI C'AATT_G
Cuts at: 0 983 2508
Size: 983 1525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1884 2508
Size: 1884 624
MslI CAynn'nnrTG
Cuts at: 0 302 2437 2508
Size: 302 2135 71
Fragments arranged by size:
2135 302 71
MspA1I CmG'CkG
Cuts at: 0 757 1393 2508
Size: 757 636 1115
Fragments arranged by size:
1115 757 636
NcoI C'CATG_G
Cuts at: 0 636 2172 2508
Size: 636 1536 336
Fragments arranged by size:
1536 636 336
NsiI A_TGCA'T
Cuts at: 0 1236 1417 2508
Size: 1236 181 1091
Fragments arranged by size:
1236 1091 181
PpuMI rG'GwC_Cy
Cuts at: 0 1089 2089 2269 2508
Size: 1089 1000 180 239
Fragments arranged by size:
1089 1000 239 180
PsiI TTA'TAA
Cuts at: 0 2020 2508
Size: 2020 488
PstI C_TGCA'G
Cuts at: 0 1289 1557 2508
Size: 1289 268 951
Fragments arranged by size:
1289 951 268
PvuII CAG'CTG
Cuts at: 0 757 1393 2508
Size: 757 636 1115
Fragments arranged by size:
1115 757 636
SacI G_AGCT'C
Cuts at: 0 1571 2508
Size: 1571 937
SapI GCTCTTCn'nnn_
Cuts at: 0 1679 2508
Size: 1679 829
SexAI A'CCwGG_T
Cuts at: 0 1734 2508
Size: 1734 774
SfcI C'TryA_G
Cuts at: 0 1285 1553 2508
Size: 1285 268 955
Fragments arranged by size:
1285 955 268
SpeI A'CTAG_T
Cuts at: 0 1633 2508
Size: 1633 875
StyI C'CwwG_G
Cuts at: 0 114 527 636 786 2172 2354 2508
Size: 114 413 109 150 1386 182 154
Fragments arranged by size:
1386 413 182 154 150 114 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 866 871 898 1431 1463 2207 2239
Size: 839 27 5 27 533 32 744 32
Cuts at: 2239 2508
Size: 269
Fragments arranged by size:
839 744 533 269 32 32 27 27
5
XbaI T'CTAG_A
Cuts at: 0 2476 2508
Size: 2476 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 598 2508
Size: 598 1910
XmnI GAAnn'nnTTC
Cuts at: 0 166 2508
Size: 166 2342
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI
DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI
MslI MspA1I NcoI NsiI PpuMI PsiI PstI PvuII
SacI SapI SexAI SfcI SpeI StyI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DrdI EaeI EagI EciI EcoNI FseI FspI
FspAI HindIII KasI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI
XmaI ZraI