Restriction map for
pFN21AE2776
(Linear) (Six-base) MAPSORT of: KIEE2776.seq Check: 2204 from: 1 to: 1215
KIEE2776.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 796 877 1215
Size: 796 81 338
Fragments arranged by size:
796 338 81
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1215
Size: 415 800
AfeI AGC'GCT
Cuts at: 0 29 1215
Size: 29 1186
AflIII A'CryG_T
Cuts at: 0 524 1215
Size: 524 691
AleI CACnn'nnGTG
Cuts at: 0 871 1215
Size: 871 344
AlwNI CAG_nnn'CTG
Cuts at: 0 627 689 1173 1215
Size: 627 62 484 42
Fragments arranged by size:
627 484 62 42
ApoI r'AATT_y
Cuts at: 0 189 293 342 414 757 1030 1215
Size: 189 104 49 72 343 273 185
Fragments arranged by size:
343 273 189 185 104 72 49
AvaI C'yCGr_G
Cuts at: 0 728 841 1215
Size: 728 113 374
Fragments arranged by size:
728 374 113
BamHI G'GATC_C
Cuts at: 0 1161 1215
Size: 1161 54
BanI G'GyrC_C
Cuts at: 0 436 862 1215
Size: 436 426 353
Fragments arranged by size:
436 426 353
BanII G_rGCy'C
Cuts at: 0 72 1215
Size: 72 1143
BbeI G_GCGC'C
Cuts at: 0 440 1215
Size: 440 775
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 1215
Size: 965 250
BglI GCCn_nnn'nGGC
Cuts at: 0 934 1215
Size: 934 281
Bme1580I G_kGCm'C
Cuts at: 0 127 648 1215
Size: 127 521 567
Fragments arranged by size:
567 521 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 677 1215
Size: 677 538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 86 1215
Size: 86 1129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 1215
Size: 94 42 1079
Fragments arranged by size:
1079 94 42
Bpu10I CC'TnA_GC
Cuts at: 0 354 1215
Size: 354 861
BsaAI yAC'GTr
Cuts at: 0 776 821 1215
Size: 776 45 394
Fragments arranged by size:
776 394 45
BsaHI Gr'CG_yC
Cuts at: 0 437 965 1215
Size: 437 528 250
Fragments arranged by size:
528 437 250
BsaWI w'CCGG_w
Cuts at: 0 388 1215
Size: 388 827
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1060 1072 1102 1134 1164 1215
Size: 1030 30 12 30 32 30 51
Fragments arranged by size:
1030 51 32 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 1142 1145 1215
Size: 625 517 3 70
Fragments arranged by size:
625 517 70 3
BseYI C'CCAG_C
Cuts at: 0 596 647 1215
Size: 596 51 568
Fragments arranged by size:
596 568 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 623 702 1068 1215
Size: 229 394 79 366 147
Fragments arranged by size:
394 366 229 147 79
BsiEI CG_ry'CG
Cuts at: 0 20 1215
Size: 20 1195
BsiHKAI G_wGCw'C
Cuts at: 0 658 1215
Size: 658 557
BsmBI CGTCTCn'nnnn_
Cuts at: 0 758 1215
Size: 758 457
Bsp1286I G_dGCh'C
Cuts at: 0 72 127 648 658 1215
Size: 72 55 521 10 557
Fragments arranged by size:
557 521 72 55 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 796 877 1215
Size: 317 479 81 338
Fragments arranged by size:
479 338 317 81
BsrDI GCAATG_nn'
Cuts at: 0 269 1215
Size: 269 946
BsrFI r'CCGG_y
Cuts at: 0 1116 1215
Size: 1116 99
BssSI C'ACGA_G
Cuts at: 0 99 915 1215
Size: 99 816 300
Fragments arranged by size:
816 300 99
BstYI r'GATC_y
Cuts at: 0 1161 1215
Size: 1161 54
BtgI C'CryG_G
Cuts at: 0 669 1215
Size: 669 546
BtsI GCAGTG_nn'
Cuts at: 0 513 658 1215
Size: 513 145 557
Fragments arranged by size:
557 513 145
DraI TTT'AAA
Cuts at: 0 1035 1215
Size: 1035 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 1215
Size: 745 470
EaeI y'GGCC_r
Cuts at: 0 17 1215
Size: 17 1198
EagI C'GGCC_G
Cuts at: 0 17 1215
Size: 17 1198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 415 1215
Size: 94 42 279 800
Fragments arranged by size:
800 279 94 42
EcoO109I rG'GnC_Cy
Cuts at: 0 724 991 1215
Size: 724 267 224
Fragments arranged by size:
724 267 224
EcoRI G'AATT_C
Cuts at: 0 293 1215
Size: 293 922
HaeII r_GCGC'y
Cuts at: 0 31 40 440 666 1215
Size: 31 9 400 226 549
Fragments arranged by size:
549 400 226 31 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1030 1062 1215
Size: 898 32 100 32 153
Fragments arranged by size:
898 153 100 32 32
KasI G'GCGC_C
Cuts at: 0 436 1215
Size: 436 779
MslI CAynn'nnrTG
Cuts at: 0 529 871 1215
Size: 529 342 344
Fragments arranged by size:
529 344 342
MspA1I CmG'CkG
Cuts at: 0 557 1215
Size: 557 658
NarI GG'CG_CC
Cuts at: 0 437 1215
Size: 437 778
NspI r_CATG'y
Cuts at: 0 528 1215
Size: 528 687
PciI A'CATG_T
Cuts at: 0 524 1215
Size: 524 691
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1215
Size: 252 963
PfoI T'CCnGG_A
Cuts at: 0 10 245 1215
Size: 10 235 970
Fragments arranged by size:
970 235 10
PmlI CAC'GTG
Cuts at: 0 776 1215
Size: 776 439
PstI C_TGCA'G
Cuts at: 0 314 714 1215
Size: 314 400 501
Fragments arranged by size:
501 400 314
PvuII CAG'CTG
Cuts at: 0 557 1215
Size: 557 658
SfcI C'TryA_G
Cuts at: 0 310 710 1215
Size: 310 400 505
Fragments arranged by size:
505 400 310
SfoI GGC'GCC
Cuts at: 0 438 1215
Size: 438 777
SmaI CCC'GGG
Cuts at: 0 730 1215
Size: 730 485
SspI AAT'ATT
Cuts at: 0 1065 1215
Size: 1065 150
StyI C'CwwG_G
Cuts at: 0 93 183 1215
Size: 93 90 1032
Fragments arranged by size:
1032 93 90
TatI w'GTAC_w
Cuts at: 0 332 1215
Size: 332 883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1134 1166 1215
Size: 1134 32 49
Fragments arranged by size:
1134 49 32
Tth111I GACn'n_nGTC
Cuts at: 0 499 964 1215
Size: 499 465 251
Fragments arranged by size:
499 465 251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 253 825 1215
Size: 249 4 572 390
Fragments arranged by size:
572 390 249 4
XmaI C'CCGG_G
Cuts at: 0 728 1215
Size: 728 487
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApoI AvaI
BamHI BanI BanII BbeI BbsI BglI Bme1580I BmrI
BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BssSI BstYI BtgI BtsI DraI DrdI EaeI
EagI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI MslI
MspA1I NarI NspI PciI PflMI PfoI PmlI PstI
PvuII SfcI SfoI SmaI SspI StyI TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BpuEI BsaI BsaBI BsiWI BsmI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII EarI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TaqII XbaI XhoI XmnI
ZraI