Restriction map for
pFN21AE2774
(Linear) (Six-base) MAPSORT of: KIEE2774.seq Check: 5221 from: 1 to: 2115
KIEE2774.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 796 877 1717 2115
Size: 796 81 840 398
Fragments arranged by size:
840 796 398 81
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 2115
Size: 415 1700
AfeI AGC'GCT
Cuts at: 0 29 2115
Size: 29 2086
AflIII A'CryG_T
Cuts at: 0 524 2115
Size: 524 1591
AhdI GACnn_n'nnGTC
Cuts at: 0 1451 2115
Size: 1451 664
AleI CACnn'nnGTG
Cuts at: 0 871 2115
Size: 871 1244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 2115
Size: 1321 32 762
Fragments arranged by size:
1321 762 32
AlwNI CAG_nnn'CTG
Cuts at: 0 627 689 1173 2115
Size: 627 62 484 942
Fragments arranged by size:
942 627 484 62
ApaI G_GGCC'C
Cuts at: 0 1552 2115
Size: 1552 563
ApoI r'AATT_y
Cuts at: 0 189 293 342 414 757 1030 1401 2115
Size: 189 104 49 72 343 273 371 714
Fragments arranged by size:
714 371 343 273 189 104 72 49
AvaI C'yCGr_G
Cuts at: 0 728 841 1534 2115
Size: 728 113 693 581
Fragments arranged by size:
728 693 581 113
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1719 1752 2115
Size: 1719 33 363
Fragments arranged by size:
1719 363 33
BamHI G'GATC_C
Cuts at: 0 1161 2009 2115
Size: 1161 848 106
Fragments arranged by size:
1161 848 106
BanI G'GyrC_C
Cuts at: 0 436 862 2115
Size: 436 426 1253
Fragments arranged by size:
1253 436 426
BanII G_rGCy'C
Cuts at: 0 72 1552 1559 1981 2115
Size: 72 1480 7 422 134
Fragments arranged by size:
1480 422 134 72 7
BbeI G_GCGC'C
Cuts at: 0 440 2115
Size: 440 1675
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 2115
Size: 965 1150
BciVI GTATCCnnnnn_n'
Cuts at: 0 1747 2115
Size: 1747 368
BclI T'GATC_A
Cuts at: 0 2090 2115
Size: 2090 25
BglI GCCn_nnn'nGGC
Cuts at: 0 934 2115
Size: 934 1181
Bme1580I G_kGCm'C
Cuts at: 0 127 648 1552 1749 2115
Size: 127 521 904 197 366
Fragments arranged by size:
904 521 366 197 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 677 2115
Size: 677 1438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 86 2115
Size: 86 2029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 1645 2115
Size: 94 42 1509 470
Fragments arranged by size:
1509 470 94 42
Bpu10I CC'TnA_GC
Cuts at: 0 354 1988 2001 2115
Size: 354 1634 13 114
Fragments arranged by size:
1634 354 114 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 2115
Size: 1342 773
BsaAI yAC'GTr
Cuts at: 0 776 821 2115
Size: 776 45 1294
Fragments arranged by size:
1294 776 45
BsaHI Gr'CG_yC
Cuts at: 0 437 965 1835 2115
Size: 437 528 870 280
Fragments arranged by size:
870 528 437 280
BsaWI w'CCGG_w
Cuts at: 0 388 1450 2115
Size: 388 1062 665
Fragments arranged by size:
1062 665 388
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1060 1072 1102 1134 1164 2115
Size: 1030 30 12 30 32 30 951
Fragments arranged by size:
1030 951 32 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 1142 1145 2094 2115
Size: 625 517 3 949 21
Fragments arranged by size:
949 625 517 21 3
BseYI C'CCAG_C
Cuts at: 0 596 647 1544 1980 2040 2115
Size: 596 51 897 436 60 75
Fragments arranged by size:
897 596 436 75 60 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 623 702 1068 1695 1854 2115
Size: 229 394 79 366 627 159 261
Fragments arranged by size:
627 394 366 261 229 159 79
BsiEI CG_ry'CG
Cuts at: 0 20 2115
Size: 20 2095
BsiHKAI G_wGCw'C
Cuts at: 0 658 1531 2115
Size: 658 873 584
Fragments arranged by size:
873 658 584
BsmI GAATG_Cn'
Cuts at: 0 1606 2115
Size: 1606 509
BsmBI CGTCTCn'nnnn_
Cuts at: 0 758 1575 2115
Size: 758 817 540
Fragments arranged by size:
817 758 540
Bsp1286I G_dGCh'C
Cuts at: 0 72 127 648 658 1531 1552 1559 1749
Size: 72 55 521 10 873 21 7 190
Cuts at: 1749 1981 2115
Size: 232 134
Fragments arranged by size:
873 521 232 190 134 72 55 21
10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 796 877 1717 1789 2115
Size: 317 479 81 840 72 326
Fragments arranged by size:
840 479 326 317 81 72
BsrBI CCG'CTC
Cuts at: 0 1973 2115
Size: 1973 142
BsrDI GCAATG_nn'
Cuts at: 0 269 2115
Size: 269 1846
BsrFI r'CCGG_y
Cuts at: 0 1116 1837 2115
Size: 1116 721 278
Fragments arranged by size:
1116 721 278
BssSI C'ACGA_G
Cuts at: 0 99 915 2115
Size: 99 816 1200
Fragments arranged by size:
1200 816 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1601 1670 2115
Size: 1601 69 445
Fragments arranged by size:
1601 445 69
BstYI r'GATC_y
Cuts at: 0 1161 2009 2115
Size: 1161 848 106
Fragments arranged by size:
1161 848 106
Bsu36I CC'TnA_GG
Cuts at: 0 1691 2115
Size: 1691 424
BtgI C'CryG_G
Cuts at: 0 669 2115
Size: 669 1446
BtsI GCAGTG_nn'
Cuts at: 0 513 658 1767 2115
Size: 513 145 1109 348
Fragments arranged by size:
1109 513 348 145
DraI TTT'AAA
Cuts at: 0 1035 1923 2115
Size: 1035 888 192
Fragments arranged by size:
1035 888 192
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 2115
Size: 745 1370
EaeI y'GGCC_r
Cuts at: 0 17 1839 2115
Size: 17 1822 276
Fragments arranged by size:
1822 276 17
EagI C'GGCC_G
Cuts at: 0 17 2115
Size: 17 2098
EarI CTCTTCn'nnn_
Cuts at: 0 2024 2115
Size: 2024 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 415 1645 2115
Size: 94 42 279 1230 470
Fragments arranged by size:
1230 470 279 94 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1624 1690 2115
Size: 1624 66 425
Fragments arranged by size:
1624 425 66
EcoO109I rG'GnC_Cy
Cuts at: 0 724 991 1445 1548 2115
Size: 724 267 454 103 567
Fragments arranged by size:
724 567 454 267 103
EcoRI G'AATT_C
Cuts at: 0 293 2115
Size: 293 1822
HaeII r_GCGC'y
Cuts at: 0 31 40 440 666 2115
Size: 31 9 400 226 1449
Fragments arranged by size:
1449 400 226 31 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1030 1062 1437 1469 1555 1587
Size: 898 32 100 32 375 32 86 32
Cuts at: 1587 2001 2033 2115
Size: 414 32 82
Fragments arranged by size:
898 414 375 100 86 82 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 436 2115
Size: 436 1679
MslI CAynn'nnrTG
Cuts at: 0 529 871 2066 2115
Size: 529 342 1195 49
Fragments arranged by size:
1195 529 342 49
MspA1I CmG'CkG
Cuts at: 0 557 1493 1984 2040 2115
Size: 557 936 491 56 75
Fragments arranged by size:
936 557 491 75 56
NaeI GCC'GGC
Cuts at: 0 1839 2115
Size: 1839 276
NarI GG'CG_CC
Cuts at: 0 437 2115
Size: 437 1678
NgoMIV G'CCGG_C
Cuts at: 0 1837 2115
Size: 1837 278
NspI r_CATG'y
Cuts at: 0 528 2115
Size: 528 1587
PciI A'CATG_T
Cuts at: 0 524 2115
Size: 524 1591
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 2115
Size: 252 1863
PfoI T'CCnGG_A
Cuts at: 0 10 245 2115
Size: 10 235 1870
Fragments arranged by size:
1870 235 10
PmlI CAC'GTG
Cuts at: 0 776 2115
Size: 776 1339
PpuMI rG'GwC_Cy
Cuts at: 0 1445 2115
Size: 1445 670
PsiI TTA'TAA
Cuts at: 0 1471 2115
Size: 1471 644
PspOMI G'GGCC_C
Cuts at: 0 1548 2115
Size: 1548 567
PstI C_TGCA'G
Cuts at: 0 314 714 2115
Size: 314 400 1401
Fragments arranged by size:
1401 400 314
PvuII CAG'CTG
Cuts at: 0 557 1493 1984 2040 2115
Size: 557 936 491 56 75
Fragments arranged by size:
936 557 491 75 56
SfcI C'TryA_G
Cuts at: 0 310 710 2115
Size: 310 400 1405
Fragments arranged by size:
1405 400 310
SfoI GGC'GCC
Cuts at: 0 438 2115
Size: 438 1677
SmaI CCC'GGG
Cuts at: 0 730 2115
Size: 730 1385
SmlI C'TyrA_G
Cuts at: 0 1357 2115
Size: 1357 758
SspI AAT'ATT
Cuts at: 0 1065 2115
Size: 1065 1050
StyI C'CwwG_G
Cuts at: 0 93 183 1340 1559 1636 2115
Size: 93 90 1157 219 77 479
Fragments arranged by size:
1157 479 219 93 90 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1691 2115
Size: 1691 424
TatI w'GTAC_w
Cuts at: 0 332 2115
Size: 332 1783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1134 1166 2115
Size: 1134 32 949
Fragments arranged by size:
1134 949 32
Tth111I GACn'n_nGTC
Cuts at: 0 499 964 1567 2115
Size: 499 465 603 548
Fragments arranged by size:
603 548 499 465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 253 825 2115
Size: 249 4 572 1290
Fragments arranged by size:
1290 572 249 4
XmaI C'CCGG_G
Cuts at: 0 728 2115
Size: 728 1387
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtsI
DraI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I KasI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PsiI
PspOMI PstI PvuII SfcI SfoI SmaI SmlI SspI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI ApaLI
AscI AseI AsiSI AvrII BbvCI BcgI BfrBI BglII
BlpI BmgBI BmtI BsaI BsaBI BsiWI BspEI BspHI
BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraIII EciI EcoICRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmeI PpiI PshAI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
SwaI XbaI XhoI XmnI ZraI