Restriction map for
pFN21AE2769
(Linear) (Six-base) MAPSORT of: KIEE2769.seq Check: 8461 from: 1 to: 1413
KIEE2769.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
Acc65I G'GTAC_C
Cuts at: 0 1356 1413
Size: 1356 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 808 1413
Size: 772 36 605
Fragments arranged by size:
772 605 36
AflIII A'CryG_T
Cuts at: 0 1163 1413
Size: 1163 250
AlwNI CAG_nnn'CTG
Cuts at: 0 174 1047 1413
Size: 174 873 366
Fragments arranged by size:
873 366 174
BanI G'GyrC_C
Cuts at: 0 26 195 318 342 1356 1413
Size: 26 169 123 24 1014 57
Fragments arranged by size:
1014 169 123 57 26 24
BanII G_rGCy'C
Cuts at: 0 379 727 989 1185 1413
Size: 379 348 262 196 228
Fragments arranged by size:
379 348 262 228 196
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 1413
Size: 66 1347
BglI GCCn_nnn'nGGC
Cuts at: 0 129 316 1413
Size: 129 187 1097
Fragments arranged by size:
1097 187 129
Bme1580I G_kGCm'C
Cuts at: 0 347 1037 1413
Size: 347 690 376
Fragments arranged by size:
690 376 347
BmrI ACTGGGnnnn_n'
Cuts at: 0 1251 1413
Size: 1251 162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1199 1413
Size: 1199 214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 862 903 1013 1413
Size: 513 349 41 110 400
Fragments arranged by size:
513 400 349 110 41
Bpu10I CC'TnA_GC
Cuts at: 0 31 1413
Size: 31 1382
BsaI GGTCTCn'nnnn_
Cuts at: 0 167 1413
Size: 167 1246
BsaAI yAC'GTr
Cuts at: 0 1164 1413
Size: 1164 249
BsaHI Gr'CG_yC
Cuts at: 0 41 1413
Size: 41 1372
BsaWI w'CCGG_w
Cuts at: 0 289 1079 1413
Size: 289 790 334
Fragments arranged by size:
790 334 289
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 1413
Size: 343 1070
BseYI C'CCAG_C
Cuts at: 0 269 1037 1069 1413
Size: 269 768 32 344
Fragments arranged by size:
768 344 269 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1328 1352 1413
Size: 1024 304 24 61
Fragments arranged by size:
1024 304 61 24
BsiHKAI G_wGCw'C
Cuts at: 0 989 1185 1413
Size: 989 196 228
Fragments arranged by size:
989 228 196
BsmI GAATG_Cn'
Cuts at: 0 611 1413
Size: 611 802
Bsp1286I G_dGCh'C
Cuts at: 0 347 379 727 989 1037 1185 1413
Size: 347 32 348 262 48 148 228
Fragments arranged by size:
348 347 262 228 148 48 32
BsrBI CCG'CTC
Cuts at: 0 1178 1413
Size: 1178 235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 606 1047 1413
Size: 606 441 366
Fragments arranged by size:
606 441 366
BstBI TT'CG_AA
Cuts at: 0 809 1413
Size: 809 604
Bsu36I CC'TnA_GG
Cuts at: 0 677 692 1413
Size: 677 15 721
Fragments arranged by size:
721 677 15
BtgI C'CryG_G
Cuts at: 0 395 1097 1413
Size: 395 702 316
Fragments arranged by size:
702 395 316
BtsI GCAGTG_nn'
Cuts at: 0 632 1413
Size: 632 781
EaeI y'GGCC_r
Cuts at: 0 1055 1413
Size: 1055 358
EarI CTCTTCn'nnn_
Cuts at: 0 88 367 463 913 1413
Size: 88 279 96 450 500
Fragments arranged by size:
500 450 279 96 88
EcoICRI GAG'CTC
Cuts at: 0 987 1183 1413
Size: 987 196 230
Fragments arranged by size:
987 230 196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 772 808 862 903 1013 1413
Size: 513 259 36 54 41 110 400
Fragments arranged by size:
513 400 259 110 54 41 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 444 1413
Size: 444 969
EcoO109I rG'GnC_Cy
Cuts at: 0 5 1413
Size: 5 1408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 1137 1169 1413
Size: 445 32 660 32 244
Fragments arranged by size:
660 445 244 32 32
HincII GTy'rAC
Cuts at: 0 632 1413
Size: 632 781
HindIII A'AGCT_T
Cuts at: 0 1326 1413
Size: 1326 87
KpnI G_GTAC'C
Cuts at: 0 1360 1413
Size: 1360 53
MscI TGG'CCA
Cuts at: 0 1057 1413
Size: 1057 356
MslI CAynn'nnrTG
Cuts at: 0 462 1413
Size: 462 951
MspA1I CmG'CkG
Cuts at: 0 1047 1069 1413
Size: 1047 22 344
Fragments arranged by size:
1047 344 22
NcoI C'CATG_G
Cuts at: 0 395 1413
Size: 395 1018
PflMI CCAn_nnn'nTGG
Cuts at: 0 973 1413
Size: 973 440
PfoI T'CCnGG_A
Cuts at: 0 529 911 1413
Size: 529 382 502
Fragments arranged by size:
529 502 382
PpuMI rG'GwC_Cy
Cuts at: 0 5 1413
Size: 5 1408
PvuII CAG'CTG
Cuts at: 0 1047 1413
Size: 1047 366
SacI G_AGCT'C
Cuts at: 0 989 1185 1413
Size: 989 196 228
Fragments arranged by size:
989 228 196
SapI GCTCTTCn'nnn_
Cuts at: 0 367 463 1413
Size: 367 96 950
Fragments arranged by size:
950 367 96
SfcI C'TryA_G
Cuts at: 0 166 1413
Size: 166 1247
StuI AGG'CCT
Cuts at: 0 821 1413
Size: 821 592
StyI C'CwwG_G
Cuts at: 0 36 275 395 998 1413
Size: 36 239 120 603 415
Fragments arranged by size:
603 415 239 120 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 213 429 1386 1413
Size: 213 216 957 27
Fragments arranged by size:
957 216 213 27
Tth111I GACn'n_nGTC
Cuts at: 0 537 1257 1413
Size: 537 720 156
Fragments arranged by size:
720 537 156
XmnI GAAnn'nnTTC
Cuts at: 0 1329 1413
Size: 1329 84
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI BanI BanII BbsI BglI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI
BstAPI BstBI Bsu36I BtgI BtsI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII HindIII KpnI MscI
MslI MspA1I NcoI PflMI PfoI PpuMI PvuII SacI
SapI SfcI StuI StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BpuEI
BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
XbaI XcmI XhoI XmaI ZraI