Restriction map for
pFN21AE2768
(Linear) (Six-base) MAPSORT of: KIEE2768.seq Check: 833 from: 1 to: 672
KIEE2768.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 97 672
Size: 97 575
AfeI AGC'GCT
Cuts at: 0 623 672
Size: 623 49
AgeI A'CCGG_T
Cuts at: 0 410 672
Size: 410 262
AlwNI CAG_nnn'CTG
Cuts at: 0 158 293 425 548 612 672
Size: 158 135 132 123 64 60
Fragments arranged by size:
158 135 132 123 64 60
ApaI G_GGCC'C
Cuts at: 0 638 672
Size: 638 34
ApoI r'AATT_y
Cuts at: 0 51 672
Size: 51 621
BanII G_rGCy'C
Cuts at: 0 310 638 672
Size: 310 328 34
Fragments arranged by size:
328 310 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 63 672
Size: 63 609
BbvCI CC'TCA_GC
Cuts at: 0 446 672
Size: 446 226
BfrBI ATG'CAT
Cuts at: 0 212 672
Size: 212 460
Bme1580I G_kGCm'C
Cuts at: 0 143 638 672
Size: 143 495 34
Fragments arranged by size:
495 143 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 672
Size: 465 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 672
Size: 378 294
Bpu10I CC'TnA_GC
Cuts at: 0 304 446 625 672
Size: 304 142 179 47
Fragments arranged by size:
304 179 142 47
BsaWI w'CCGG_w
Cuts at: 0 410 672
Size: 410 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 397 427 451 481 672
Size: 307 30 60 30 24 30 191
Fragments arranged by size:
307 191 60 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 672
Size: 328 344
BseYI C'CCAG_C
Cuts at: 0 38 605 672
Size: 38 567 67
Fragments arranged by size:
567 67 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 672
Size: 580 92
BsiHKAI G_wGCw'C
Cuts at: 0 310 394 410 672
Size: 310 84 16 262
Fragments arranged by size:
310 262 84 16
BsmI GAATG_Cn'
Cuts at: 0 214 329 672
Size: 214 115 343
Fragments arranged by size:
343 214 115
Bsp1286I G_dGCh'C
Cuts at: 0 143 310 394 410 638 672
Size: 143 167 84 16 228 34
Fragments arranged by size:
228 167 143 84 34 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 97 672
Size: 97 575
BsrFI r'CCGG_y
Cuts at: 0 410 672
Size: 410 262
BstEII G'GTnAC_C
Cuts at: 0 123 672
Size: 123 549
EcoICRI GAG'CTC
Cuts at: 0 308 672
Size: 308 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 672
Size: 378 294
EcoO109I rG'GnC_Cy
Cuts at: 0 155 634 672
Size: 155 479 38
Fragments arranged by size:
479 155 38
EcoRI G'AATT_C
Cuts at: 0 51 672
Size: 51 621
HaeII r_GCGC'y
Cuts at: 0 625 672
Size: 625 47
HindIII A'AGCT_T
Cuts at: 0 497 672
Size: 497 175
MslI CAynn'nnrTG
Cuts at: 0 100 672
Size: 100 572
MspA1I CmG'CkG
Cuts at: 0 433 672
Size: 433 239
NsiI A_TGCA'T
Cuts at: 0 214 672
Size: 214 458
NspI r_CATG'y
Cuts at: 0 603 672
Size: 603 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 293 672
Size: 293 379
PspOMI G'GGCC_C
Cuts at: 0 634 672
Size: 634 38
SacI G_AGCT'C
Cuts at: 0 310 672
Size: 310 362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 672
Size: 307 32 333
Fragments arranged by size:
333 307 32
Tth111I GACn'n_nGTC
Cuts at: 0 121 425 672
Size: 121 304 247
Fragments arranged by size:
304 247 121
XmnI GAAnn'nnTTC
Cuts at: 0 109 352 672
Size: 109 243 320
Fragments arranged by size:
320 243 109
Enzymes that do cut:
AarI AfeI AgeI AlwNI ApaI ApoI BanII BbsI
BbvCI BfrBI Bme1580I BmrI BpmI Bpu10I BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BstEII EcoICRI Eco57MI EcoO109I EcoRI HaeII HindIII MslI
MspA1I NsiI NspI PflMI PspOMI SacI TstI Tth111I
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoNI
EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI XbaI XcmI
XhoI XmaI ZraI